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UI-E-EJ0

Identifiers
BioSample: SAMN00170185; EST: LIBEST_010290
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuefetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid
development stagefetal and adult
lab hostDH10B (Life Technologies) (T1 phage resistant)
tissueeye
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
v_typePlasmid
re_1EcoR I
re_2Not I
Description

UI-E-EJ0 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

Submission
Children's Memorial Research Center, Soares, MB; 2002-02-27
Accession:
SAMN00170185
ID:
170185

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