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1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo (Soares4).
Nucleotide
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