NCI_CGAP_FE1

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Result Filters

Organism: Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo

Attributes

tissue	Cell lines
development stage	Adult
lab host	DH10B (Life Technologies)
tissue	Chondrosarcoma
vector	pT7T3-Pac (Pharmacia) with a modified polylinker
v_type	Plasmid
re_1	EcoR I
re_2	Not I

Description: NCI_CGAP_FE1 is a normalized cDNA library derived from a pool of mRNA obtained from 3 cell lines from grade II chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCTACGGAC. The cell lines were provided by Dr James Martin from the University of Iowa.