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UI-R-FJ0

Identifiers
BioSample: SAMN00171765; EST: LIBEST_012128
Organism
Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Attributes
strainSprague-Dawley
tissueembryo
development stageembryo
lab hostDH10B (Life Technologies) (T1 phage resistant)
vectorpYX-Asc
v_typePlasmid
re_1EcoR I
re_2Not I
Description

UI-R-FJ0 is a cDNA library containing the following tissue(s): rat embryo. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant)

Submission
Children's Memorial Research Center, Soares, MB; 2002-11-14
Accession:
SAMN00171765
ID:
171765

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