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RIKEN full-length enriched, adult male testis

Identifiers
BioSample: SAMN00157070; EST: LIBEST_002511
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6J
sexmale
tissuetestis
development stageadult
lab hostSOLR
re_1XhoI
re_2BamHI
Description

cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAGCGGCCGCAATTAATTCTCGAGTTAATTAAATTAATCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites.

Submission
The Institute of Physical and Chemical Research (RIKEN), Yoshihide Hayashizaki; 1999-10-29
Accession:
SAMN00157070
ID:
157070

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