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BioSample: SAMN00171717; EST: LIBEST_012072
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
tissueAlveolar Macrophage
development stageAdult
lab hostDH10B (Life Technologies)
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
re_1EcoR I
re_2Not I

NCI_CGAP_FT1 is a normalized cDNA library constructed from a pool of 81 RNA samples from Alveolar Macrophages challenged with different treatments. The mRNA samples were a mixture of these conditions (times refer to incubations following isolation by bronchoalveolar lavage) (some normal donor macrophages were cultured in some of the conditions, other donor macrophages in different conditions). The mRNA samples were pooled for library construction. Control 0 hours; control 3 hours; control 24 hours; LPS 100 ng/ml, 3 hours; LPS 100 ng/ml, 24 hours; PMA 10 ng/ml, 3 hours; PMA 10 ng/ml, 24 hours; Klebsiella moi 10, 3 hours; Klebsiella moi 10, 24 hours; Staph aureus moi 10, 3 hours; Staph aureus moi 10, 24 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 3 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 24 hours; wt adenovirus moi 500, 3 hours; wt adenovirus moi 500, 24 hours; Ad vector + LPS 3 hours; Ad vector + LPS 24 hours; wt adenovirus + LPS 3 hours; wt adenovirus + LPS 24 hours. The library was normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGCCATGCCG. The tissue was provided by Dr. Gary W. Hunninghake of the University of Iowa.

National Cancer Institute / NIH, Daniela S. Gerhard, Ph.D.; 2002-11-01

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