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NIH_BMAP_GW0

Identifiers
BioSample: SAMN00173435; EST: LIBEST_013963
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6
tissuewhole eye
development stageembryo 15.5,16.5,17.5,18.5 dpc
lab hostDH10B (T1 phage resistant)
tissueEye
vectorpYX- Asc
v_typePlasmid
re_1EcoR I
re_2Not I
Description

The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CTGCGTCCTC. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

Submission
, Robert Strausberg, Ph.D.; 2003-07-07
Accession:
SAMN00173435
ID:
173435

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