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An unidirectional cDNA library generated from [Shoot-root transition zone from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public
Nucleotide
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