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UI-R-E1

Identifiers
BioSample: SAMN00155868; EST: LIBEST_001284
Organism
Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Attributes
strainSprague-Dawley
development stageadult
lab hostDH10B (Life Technologies)
vectorpT7T3D-PacI
v_typePhagemid
re_1Not I
re_2Eco RI
Description

The UI-R-E1 library is a subtracted library derived from the UI-R-E0 library. The UI-R-E0 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-E1) was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-E0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-E0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-E1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

Submission
Children's Memorial Research Center, Soares, MB; 1998-05-07
Accession:
SAMN00155868
ID:
155868

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