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Soares_mammary_gland_NbMMG

Identifiers
BioSample: SAMN00155466; EST: LIBEST_000868
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6J
sexmale
tissuemammary gland
development stage4 weeks
lab hostDH10B
tissuemammary gland
vectorpT7T3D-PacI
v_typephagemid (ampicillin resistant)
re_1Not I
re_2Eco RI
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATGGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Minoru Ko, Wayne State Univ. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

Submission
Washington University School of MedicineP, Marra M/Mouse EST Project; 1997-05-14
Accession:
SAMN00155466
ID:
155466

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