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NIH_BMAP_M_S4

Identifiers
BioSample: SAMN00158298; EST: LIBEST_003742
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6J
development stage27-32 days
lab hostDH10B (Life Technologies)
vectorpT7T3D-PacI
v_typePhagemid
re_1Not I
re_2Eco RI
Description

The NIH_BMAP_M_S4 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S4, NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S4) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with a pool of the NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 libraries in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S4 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

Submission
, Robert Strausberg, Ph.D.; 2000-02-22
Accession:
SAMN00158298
ID:
158298

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