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SLX-19432.i729_i518.HK3H3BBXY.s_2

Identifiers
BioSample: SAMEA14418509; SRA: ERS12024071
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
age74 to 80
cell typehematopoietic stem cell
genotypewild type genotype
tissuebone marrow
phenotypeaged
sample nameE-MTAB-11359:SLX-19432.i729_i518.HK3H3BBXY.s_2
ENA first public2022-05-20
ENA last update2022-05-20
ENA-CHECKLISTERC000011
External IdSAMEA14418509
INSDC center aliasHelmholtz Munich
INSDC center nameHelmholtz Munich
INSDC first public2022-05-20T16:17:48Z
INSDC last update2022-05-20T16:17:48Z
INSDC statuspublic
Submitter IdE-MTAB-11359:SLX-19432.i729_i518.HK3H3BBXY.s_2
broker nameArrayExpress
common namehouse mouse
immunophenotypeLin− Sca-1+ cKit+
inferred cell typehematopoietic stem cell
protocolSmart-seq2
single cell well qualityOK
Description

Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

BioProject
PRJEB52992 Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
Retrieve all samples from this project

Submission
EBI; 2022-05-21
Accession:
SAMEA14418509
ID:
28576234

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