Box 3Primer/probe reactivity

Reactivity scores characterize the reactivity between a primer/probe and an allele for primers and probes and are listed below:

  • Positive: probe anneals with allele.
  • Weak: probe anneals sometimes with allele.
  • Unclear: annealing cannot be predicted, no empirical information, allele has not been sequenced at the annealing position.
  • Negative: probe does not anneal.
    dbMHC calculates primer/probe reactivity based on sequence similarity only and does not take into account laboratory experimental conditions such as magnesium concentration, temperature, etc.

From: Chapter 11, The Major Histocompatibility Complex Database, dbMHC

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