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National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington (DC): National Academies Press (US); 2011.

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Guide for the Care and Use of Laboratory Animals. 8th edition.

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AddendumGuide for the Care and Use of Laboratory Animals Eighth Edition


  1. Page 22. Original sentence: “Personnel training should include information on laboratory animal allergies, preventive control measures and proper techniques for working with animals (Gordon et at. 1997; Schweitzer et al. 2003; Thulin et al. 2002).” “…early recognition and reporting of allergy symptoms” was added to clarify the guidance and reflect the cited references.
  2. Page 31. Original sentence: “They should therefore be used, when available, for all animal-related procedures (NIH 2008; USDA 1997b).” The NIH reference was removed because it applies only to the NIH intramural animal research program.
  3. Page 32. Original sentence: “Principal investigators conducting field research should be knowledgeable of relevant zoonotic diseases, associated safety issues, and, when working in an international environment, any local laws or regulations that apply.” Compliance with laws and regulations applies to field investigations irrespective of location, so “when working in an international environment” and “local” were deleted.
  4. Page 32. Original sentence: “Appropriate veterinary input is needed for projects involving capture, individual identification, sedation, anesthesia, surgery, recovery, holding, transportation, release, or euthanasia.” Beginning of sentence changed to “Veterinary input may be needed…” to clarify the intent of the sentence.
  5. Page 43. Original sentence: “The ambient temperature range in which thermoregulation occurs without the need to increase metabolic heat production is called the thermoneutral zone (TNZ) and is bounded by the upper (UCT) and lower critical temperatures (LCT).” The phrase “or activate evaporative heat loss mechanisms” and reference to Gordon 2005 were added to provide a more complete definition of thermoneutral zone.
  6. Page 45. Original sentence: “In climates where it is difficult to provide a sufficient level of environmental relative humidity, animals should be closely monitored for negative effects such as excessively flaky skin in birds and mammals, ecdysis (molting) difficulties in reptiles, and desiccation stress in semiaquatic amphibians.” Because the significance of excessive flaky skin varies among species, “in birds and mammals” was deleted.
  7. Page 55. Original sentence: “Thus there is no ideal formula for calculating an animal’s space needs based only on body size or weight.” The phrase “and readers should take the performance indices discussed in this section into consideration when utilizing the species-specific guidelines presented in the following pages” was added to further clarify the intent of this section.
  8. Pages 57–63. The phrase “the interpretation of this table should take into consideration the performance indices described in the text beginning on page 55” was added as a footnote to tables as additional guidance to their interpretation. Additionally, the symbol “≥” was restored (due to mistaken deletion) in Table 3.2 (mice in groups; rats in groups; hamsters; guinea pigs) and added to Tables 3.5 (group 8) and 3.6 (sheep and goats)
  9. Page 69. Original sentence: “Cedar shavings are not recommended because they emit aromatic hydrocarbons that induce hepatic microsomal enzymes and cytotoxicity (Torronen et al. 1989; Weichbrod et al. 1986, 1988).” Text mistakenly deleted was restored to the end of the sentence: “and have been reported to increase the incidence of cancer (Jacobs and Dieter 1978; Vlahakis 1977).”
  10. Page 107. Original sentence: “The Centers for Disease Control and Prevention enforces regulations to prevent the introduction, transmission, or spread of communicable diseases and regulate the importation of any animal or animal product capable of carrying a zoonotic disease.” Because USDA also has jurisdiction over imports, it was added to the sentence.
  11. Page 107. Original sentence: “The US Fish and Wildlife Service regulates importation/exportation and interstate trade of wild vertebrate and invertebrate animals and their tissues.” Because US Fish and Wildlife Services does not regulate interstate trade except for species listed under the Endangered Species Act, “and interstate trade” was deleted
  12. Page 120. Original sentence: “Additional care might be warranted, including long-term administration of parenteral fluids (FBR 1987), analgesics and other drugs; and care of surgical incisions.” Because this is now standard veterinary medical practice and the cited reference is no longer in print, the reference was removed.
  13. Page 120. Original sentence: “In general, unless the contrary is known or established, it should be considered that procedures that cause pain in humans may also cause pain in vertebrate species (IRAC 1985).” For consistency with the text of the U.S. Government Principles the phrase “The U.S. Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training (see Appendix B) state that…” was added and the words “in vertebrate species” were replaced with “in other animals.”
  14. Page 143. Original phrase: “Vibration, especially if animals are housed directly above the washing facility,” was expanded because animals housed below or adjacent to the washing facility are also subject to potential vibration.
  15. Added references: pages 163; 169; 179; 182; 192.



Page numbers reflect placement of revisions in this report.

Copyright © 2011, National Academy of Sciences.
Bookshelf ID: NBK54040


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