Conserved Protein Domain Family
hCaCC

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TIGR00868: hCaCC 
calcium-activated chloride channel protein 1
The Epithelial Chloride Channel (E-ClC) Family (TC 1.A.13). Mammals have multiple isoforms of epithelial chloride channel proteins. The first member of this family to be characterized was a respiratory epithelium, Ca 2+-regulated, chloride channel protein isolated from bovine tracheal apical membranes. It was biochemically characterized as a 140 kDa complex. The purified complex when reconstituted in a planar lipid bilayer behaved as an anion-selective channel. It was regulated by Ca 2+ via a calmodulin kinase II-dependent mechanism. When the cRNA was injected into Xenopus oocytes, an outward rectifying, DIDS-sensitive, anion conductance was measured. A related gene, Lu-ECAM, was cloned from the bovine aortic endothelial cell line, BAEC. It is expressed in the lung and spleen but not in the trachea. Homologues are found in several mammals, and at least three paralogues(hCaCC-1-3) are present in humans, each with different tissue distributions. [Transport and binding proteins, Anions]
Statistics
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PSSM-Id: 129946
Aligned: 4 rows
Threshold Bit Score: 1665.79
Created: 7-Oct-2014
Updated: 14-Jul-2023
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
P54281     1 MVPRLTVILFLTLHLLPGMK-SSMVNLINNGYDGIVIAINPSVPEDEKLIQNIKEMVTEASTYLFHATKRRVYFRNVSIL 79  cattle
AAF00077   1 MGSFRSSLFILVLHLLEGAQsNSLIQLNGNGYEGIVIAIDPNVPEDERLIQNIKDMVTKASPYLFEATEKRFYFKNVAIL 80  pig
AAD48398   1 MGLFRGFVFLLVLCLLHQSN-TSFIKLNNNGFEDIVIVIDPSVPEDEKIIEQIEDMVTTASTYLFEATEKRFFFKNVSIL 79  human
AAC35003   1 MVPGLQVLLFLTLHLLQNTE-SSMVHLNSNGYEGVVIAINPSVPEDERLIPSIKEMVTQASTYLFEASQGRVYFRNISIL 79  house mouse
P54281    80 IPMTWKSKSEYLMPKQESYDQAEVIVANPYLKHGDDPYTLQYGRCGEKGQYIHFTPNFLLTNNLPIYGSRGRAFVHEWAH 159 cattle
AAF00077  81 IPASWKAKPEYVKPKLETYKNADVVVTEPNPPENDGPYTEQMGNCGEKGEKIYFTPDFVAGKKVLQYGPQGRVFVHEWAH 160 pig
AAD48398  80 IPENWKENPQYKRPKHENHKHADVIVAPPTLPGRDEPYTKQFTECGEKGEYIHFTPDLLLGKKQNEYGPPGKLFVHEWAH 159 human
AAC35003  80 VPMTWKSKPEYLMPKRESYDKADVIVADPHLQHGDDPYTLQYGQCGDRGQYIHFTPNFLLTDNLRIYGPRGRVFVHEWAH 159 house mouse
P54281   160 LRWGIFDEYNGDQPFYISrRNTIEATRCSTHITGTNVIVKCQGGSCITRPCRRDSQTGLYEAKCTFIPEKSQTARESIMF 239 cattle
AAF00077 161 LRWGVFNEYNNEQKFYLS-NKKEQPVICSAAIRGTNVLPQCQGGSCVTKPCRADRVTGLFQKECEFIPDPQQSEKASIMF 239 pig
AAD48398 160 LRWGVFDEYNEDQPFYRAkSKKIEATRCSAGISGRNRVYKCQGGSCLSRACRIDSTTKLYGKDCQFFPDKVQTEKASIMF 239 human
AAC35003 160 LRWGVFDEYNVDQPFYMSrKNTIEATRCSTRITGTNVVHNCERGNCVTRACRRDSKTRLYEPKCTFIPDKIQTAGASIMF 239 house mouse
P54281   240 MQSLHSVTEFCTEKTHNVEAPNLQNKMCNGKSTWDVIMNSTDFQNTSPMTEMnpPTQPTFSLLKSKQRVVCLVLDKSGSM 319 cattle
AAF00077 240 AQSIDTVVEFCKEKNHNKEAPNDQNQKCNLRSTWEVIQDSEDFKKTTPMTTQ--PPAPTFSLLQIGQRIVCLVLDKSGSM 317 pig
AAD48398 240 MQSIDSVVEFCNEKTHNQEAPSLQNIKCNFRSTWEVISNSEDFKNTIPMVTP--PPPPVFSLLKIRQRIVCLVLDKSGSM 317 human
AAC35003 240 MQNLNSVVEFCTEKNHNAEAPNLQNKMCNRRSTWDVIKTSADFQNAPPMRGTeaPPPPTFSLLKSRRRVVCLVLDKSGSM 319 house mouse
P54281   320 SSEDRLFRMNQAAELFLIQIIEKGSLVGMVTFDSVAEIRNNLTKITDDNVYENITANLPQEANGGTSICRGLKAGFQAII 399 cattle
AAF00077 318 TVGGRLKRLNQAGKLFLLQTVEQGAWVGMVAFDSAAYVKSELVQINSAAERDALARSLPTAASGGTSICSGLRSAFTVIK 397 pig
AAD48398 318 GGKDRLNRMNQAAKHFLLQTVENGSWVGMVHFDSTATIVNKLIQIKSSDERNTLMAGLPTYPLGGTSICSGIKYAFQVIG 397 human
AAC35003 320 DKEDRLIRMNQAAELYLTQIVEKESMVGLVTFDSAAHIQNYLIKITSSSDYQKITANLPQQASGGTSICHGLQAGFQAIT 399 house mouse
P54281   400 QSQQsTSGSEIILLTDGEDNEIHSCIEEVKQSGVIIHTIALGPSAAKELETLSDMTGGHRFYAN--KDINGLTNAFSRIS 477 cattle
AAF00077 398 KKYP-TDGSEIVLLTDGEDNTISACFPEVKQNGAIIHTVALGPSAAKELEELSQMTGGLQTYASdqAENNGLIDAFGALS 476 pig
AAD48398 398 ELHSqLDGSEVLLLTDGEDNTASSCIDEVKQSGAIVHFIALGRAADEAVIEMSKITGGSHFYVSdeAQNNGLIDAFGALT 477 human
AAC35003 400 SSDQsTSGSEIVLLTDGEDNGIRSCFEAVSRSGAIIHTIALGPSAARELETLSDMTGGLRFYAN--KDLNSLIDAFSRIS 477 house mouse
P54281   478 SRSGSITQQTIQLESKALAITEKKWVNGTVPVDSTIGNDTFFVVTWT--IKKPEILLQDPKGKkyKTSDFKEDKLNiHSA 555 cattle
AAF00077 477 SGNRAASQRSIQLESQGLTLQNNEWMNGTVVVDSTVGKDTLFLITLErkFLSPIPFFGVPSGR--SQDSFLVGKHN-KMA 553 pig
AAD48398 478 SGNTDLSQKSLQLESKGLTLNSNAWMNDTVIIDSTVGKDTFFLITWN--SLPPSISLWDPSGT--IMENFTVDATS-KMA 552 human
AAC35003 478 STSGSVSQQALQLESKAFDVRAGAWINGTVPLDSTVGNDTFFVITWM--VKKPEIILQDPKGKkyTTSDFQDDKLNiRSA 555 house mouse
P54281   556 RLRIPGIAETGTWTYSLLNNhAspQILTVTVTTRARSPTTPPVTATAHMSQNTAHYPSPVIVYAQVSQGFLPVLGINVTA 635 cattle
AAF00077 554 YFQVPGTAKVGMWKYSLQAS-S--QTLTLTVSSRRSSATLPPVTVTSKMNKDTGKFPSPMVVYTKIHQGTLPILRAKVTA 630 pig
AAD48398 553 YLSIPGTAKVGTWAYNLQAK-AnpETLTITVTSRAANSSVPPITVNAKMNKDVNSFPSPMIVYAEILQGYVPVLGANVTA 631 human
AAC35003 556 RLQIPGTAETGTWTYSITG--TksQLITMTVTTRARSPTMEPLLATAHMSQSTAQYPSRMIVYARVSQGFLPVLGANVTA 633 house mouse
P54281   636 IIETEDGHQVTLELWDNGAGADTVKNDGIYSRYFTDYRGNGRYSLKVHAEARNNTARLSLRQPQNKALYIPGYIENGKII 715 cattle
AAF00077 631 LIESENGKTVTLELLDNGAGADATKNDGIYSRYFTAYDANGRYSVKVWALGGVNTPRRRAPPLWSGAMYIRGWIENGEIK 710 pig
AAD48398 632 FIESQNGHTEVLELLDNGAGADSFKNDGVYSRYFTAYTENGRYSLKVRAHGGANTARLKLRPPLNRAAYIPGWVVNGEIE 711 human
AAC35003 634 LIEAEHGHQVTLELWDNGAGADTVKNDGIYTRYFTDYHGNSRYSLKVRVQAQRNKTRLSLRQ-KNKSLYIPGYVENGKIV 712 house mouse
P54281   716 LNPPRPEVKDDLAKAEIEDFSRLTSGGSFTVSGAPP-GNHPSVLPPNKIIDLEAKFKEDHIQ-LSWTAPANVLDKGKANS 793 cattle
AAF00077 711 WNPPRPDINKDDLQGKQVCFSRTASGGSFVASDVPK-SPIPDLFPPCKITDLKAGIQGDNLInLTWTAPGDDYDHGRADR 789 pig
AAD48398 712 ANPPRPEID-EDTQTTLEDFSRTASGGAFVVSQVPS-LPLPDQYPPSQITDLDATVHEDKII-LTWTAPGDNFDVGKVQR 788 human
AAC35003 713 LNPPRPDVQEEAIEATVEDFNRVTSGGSFTVSGAPPdGDHARVFPPSKVTDLEAEFIGDYIH-LTWTAPGKVLDNGRAHR 791 house mouse
P54281   794 YIIRISKSFLDLQKDFDNATLVNTSSLKPKEAGSDENFEFKPEPFRIENGTNFYIAVQAINEANLTSEVSNIAQ 867 cattle
AAF00077 790 YIIRISTNILDLRDKFNDSVQVNTTDLIPKEANSEEVFVFKPEGIPFTNGTDLFIAVQAVDKTNLKSEISNIAQ 863 pig
AAD48398 789 YIIRISASILDLRDSFDDALQVNTTDLSPKEANSKESFAFKPENISEENATHIFIAIKSIDKSNLTSKVSNIAQ 862 human
AAC35003 792 YIIRMSQHPLDLQEDFNNATLVNASSLIPKEAGSKETFKFKPETFKIANGIQLYIAIQADNEASLTSEVSNIAQ 865 house mouse
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