Conserved Protein Domain Family
GCP_N_terminal
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pfam17681: GCP_N_terminal 
Gamma tubulin complex component N-terminal
This is the N-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Functional studies have shown that the N-terminal domain defines the functional identity of GCPs, suggesting that all GCPs are incorporated into the helix of gamma-tubulin small complexes (gTURCs) via lateral interactions between their N-terminal domains. Thereby, they define the direct neighbors and position the GCPs within the helical wall of gTuRC. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains. In addition to the conserved sequences, the N-terminal domains carry specific insertions of various sizes depending on the GCP, i.e. internal insertions or N-terminal extensions. These insertions may equally contribute to the function of individual GCPs as they have been implied in specific interactions with regulatory or structural proteins. For instance, GCP6 carries a large internal insertion phosphorylated by Plk4 and containing a domain of interaction with keratins, whereas the N-terminal extension of GCP3 interacts with the recruitment protein MOZART1.
Links
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Statistics
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PSSM-Id: 465456
Aligned: 166 rows
Threshold Bit Score: 102.75
Created: 26-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Q4WSG8       169 FHALFRLgL-GWNSVLFRYNKqRKAf--eRVSDNIRVsgvsLAVVN---GLTEEVLRCGTAMYNLRDFARR---T-RIKP 238  Aspergillus fu...
XP_011124834 199 VYCLLHL-VkGWDSMLFKFNE--AN---------MEFea-tREGLRlsgTLISHYLKCGNRLRALQAFAKTv---yADIG 262 
GAD96537     178 FQSLFRL-GlGWNSIFFRYNE-QKRif-dKELRDVRIsgvsLPAVD---GVIRDISQCGTGLQRIRAFVRT---N-PAVS 247 
CEO58992     174 IRSLLRLgL-GWSSSFFRYNEqTKTf--eRCMNDVRVsgvsLAMLD---SVISSVLQCGTNMQHIRAFVKN---A-PTKP 243 
CDM28698     174 IRSLLRLgL-GWSSAFFRYDQ-RTNqferCLKDNIRVsgisLMALE---NVIENVVQCGTNMQRLRLFAQDppanyRELS 248 
KGO68243     174 IRSLLRLgL-GWSSAFFRYDPhTNQf--eRHLNNIRIsgisLLALE---NMIQSVVQCGTKMQHMRMFAQD---P-PAKC 243 
EKV06197       8 IRSLLRLgL-GWSSAFFRYDQrTNEf--kRHLKDIRVsgtsSMVLE---NVIEHVAQCGTNMQRMRLFAQD---P-PAKC 77  
KKK20996     177 FQSLFRLgL-GWSSMLFRYNE-RRQkf-eNILKHTRIsgisLVTLD---GLINEISLCGNNMQKVRAFVEKaptK-SVEA 249 
CEN62609     174 FQSLFRLgL-GWSSMLFRYND-RQQkf-eKVIKDLRIsgvsALALN---GLMDEVLQCGTNMQRIRAFIDRvpfK-SAEP 246 
Q5BEM5       174 FQSLFRVgL-GWSSMLFRFNK-QRQkf-eKVVKDIRIsgvsTLALS---GIIDEMLQCGNNMQRVRTFIgrvptA-AAEP 246  Aspergillus ni...

Q4WSG8       239 EGFSaRSaFASAVAMiIYTLEKQIINYFKEVSSLIQIMTLFQRCGDLVGALSNIVEAVQKAASDAQVISAVIEk-AAH-Y 316  Aspergillus fu...
XP_011124834 263 SCCKtRVgLAHCIQIiTVAVQHRLTVLFTSIQSILQLDDIIKVPTMILEALLKIVERVKDTRLDFQLLSAIFEqiQRSeY 342 
GAD96537     248 DQPTaLStLAAAVAIlVYSLEKQLSERYYQQGSLLQIQTLFQRCGDLVGTLVNILDGVEKVKSEGEVVSTVLAk-CDH-F 325 
CEO58992     244 KDLFsLFtLRGTVAIiIYNLEQQILLYSQNIVSVLRVMTLFQRCGDLISALADVVNMAEKAKSDAQVISAVIEr-ATV-F 321 
CDM28698     249 TFFT----LTSTVEIiIFNLEQQISDHSKNIASLLQIKALFHRCGDVVGVLAAIVGAAQRAVSDAQVISIVVEr-AAF-F 322 
KGO68243     244 KELPaLFtLSSTVAViIINLEQQISDHSKNIASLLQIKALFHRCGDLIGVLAGIVGAAQKAVSDAQVMSIVTEr-AAF-F 321 
EKV06197      78 RKLSaLFtLSSTVAViIFNLEQQISDQSKSIASLLQIKAMFHRCGDLIGVLAAVVGAAQKAVSDAQAMSIVVEr-AAF-F 155 
KKK20996     250 SSLP-AF-STAASVV-IFTLERQLVKVSKHLTSLLQIKALFQRCGELVLVLANMVEAVEKARSEAHIISSILKl-AAH-Y 324 
CEN62609     247 SVLS-AF-STTASAI-IYTLEKQLLSCAKHINSALQVRALFQQCGELVGALSNMVGIVERAGSEAQVISSVFKl-AAH-Y 321 
Q5BEM5       247 SALS-AF-STAASVI-VYTLEKQLLHSFKQISSVLQIRALFQRCAELIGVLVNMMDAVETAGSEARIISSVFKl-AAH-Y 321  Aspergillus ni...

Q4WSG8       317 SEAfgSMENLFRELVARVIAPWLQYVEAWVGlRTETpaLvDLAakSE-GFVAVERYEgra-gsRSMKASSLQY-RYHPEQ 393  Aspergillus fu...
XP_011124834 343 KSD--WLRYLLLLMLQRITRPWLTMVSKAIGiTVEKtpFvDDIfeDEgFFIELGDPVl----qEGEEEPHTDL-IFAPSN 415 
GAD96537     326 SQRfvWMADLLSELTFRVTQPWLGLVETWLGlRGEErgLpELT--SN-GFIEAEYFEdptgrk--tepkivDY-IYHPEM 399 
CEO58992     322 THMfgWMENLLQEIVFRVTSPWFEFVERWIGlRSDEgaLsELVasGR-MFVQLEHHHdptkfk--tgsertEY-IYVDAH 397 
CDM28698     323 AQKfgWIENLVHEIVIRVTQPWFGFIETWIGlRSEDaaLkEYMtnDK-SFVRLETYDrgkfktGPARI---DY-IYQAEH 397 
KGO68243     322 AQQfgWMENLVHEIVIRVTRPWFKFIETWIGlRPEDtaIkASMasGK-TFVRLDTHDavklkt---rpariDH-IYQEDH 396 
EKV06197     156 AQKfgWMENLLHETVIRVTRPWFKFIENWIGlTPEEtaLtASMasGK-TFVRLDTHDaa---kIKTGLARIDH-MYQADH 230 
KKK20996     325 SQIygHMEALFREIVFRVVGPWLTHVEFWVA-FG----QkHPH--RS----NKGKLLsqe---------rlEYsSYLPDQ 384 
CEN62609     322 SHShgQMEDLFREIVFRVAGPWLDYVESWIGfRPETaaSvERLasGK-SFVYFEKPEergrlqSQERL---DY-VYLPDQ 396 
Q5BEM5       322 AQIygQMESLFREIVFKVAQPWLTYVETWIGfRPETsaSiELLtnGR-SFVSLEKSEsngkis---sqerhEY-AYLPEQ 396  Aspergillus ni...

Q4WSG8       394 MPTFIPAEQADLIFETGRSLRLLRRYhpqhpianda-iltkdspnlrCAGswsdierIQKKAQqy-etQLrseilkysqg 471  Aspergillus fu...
XP_011124834 416 IPSFLMLEEAERVHETSRGLRLLRRFhpdhpllsg---hgvervkmrWSfswdelqaID-LEAaahekRLqlamqafnlt 491 
GAD96537     400 MPSFIPEQQARLIFESGRSLRILKQShprhpigrddilrttsppalhCAIsfqdierIQDKASdy-ehRLrseilryirg 478 
CEO58992     398 MPSFIPKDQARTIFESGRSLRLLKRSdphhpiarhdvlpgaknlrlyCATtwsdierIQRQANeh-eaSLrheilkyhrg 476 
CDM28698     398 MPSFIPADQARSIFECGRSLQLLKRShprhpiarrdvlsrigglhlhCATtwagieqIQTKAQey-esRLraeikkyhqg 476 
KGO68243     397 MPSFIPADQARSIFESGRSLQLLKRShprhpiarrdvllrtgglhlhCATtwadieqIQTKAQey-esSLraeikryhkg 475 
EKV06197     231 MPSFIPADQARSIFESGRSLQLLKRShprhpiarpdvlfrtgglhlhCATtwagiehIQTKAQey-esILraeikryqdg 309 
KKK20996     385 MPSFVPPDQAQLIFESGRSLRLLTRShphhplagdr-ipslnlpslrCAStwadlerIQAKARey-etRLraeilkynrk 462 
CEN62609     397 MPSFVPPDQAQLIFESGRSLRLLKRShpnhplarr----ateipslsGAGtwaelerIQTKARdy-enRLraeirkynsg 471 
Q5BEM5       397 MPSFVPPDQAYLIYESGRSLRLLKRYhphhplageq--vridspklaCAGtwaelerIQMKARdy-eaRLraevlkynrn 473  Aspergillus ni...

Q4WSG8       472 qrvgqvemdkvl-------aeeqmqkfvsagddpaispafELf-dIDDSKhttgllasdsslendelgplfdastlevsr 543  Aspergillus fu...
XP_011124834 492 krsrfsrltdhpqlggndgfsfdfigkseeeikaslaaslEN---MN-FEpqrqfgfegaaddklgnealtve----pit 563 
GAD96537     479 dsseakcllp----------anggdirdvemaddpikstfEIi-dVDDARhvtglladssslesdklyriltgsdgldie 547 
CEO58992     477 hnskqpae--------------ldtpasrqpscgnlngtyELf-dLDDEKiisgsvvdqralvndkinqllektr-epas 540 
CDM28698     477 daglqdsssn-----------vvtqtvdqtantevtttafQLf-dIDDEKndsgpvtdykalskdglkrlldkar-gfea 543 
KGO68243     476 dtglqdppsn-----------vithgvdktadkevttaafELf-eIDDEKnaswpvtdhralsksdfkqlldkar-rsdi 542 
EKV06197     310 dadlqdspsn-----------vasrdvghtaetevttaafELfdlDEknasgpvtdhkalskddfkhlldka---rgfei 375 
KKK20996     463 elleekk----------------dskvsdkvdrekfpdsfALf-dIDDERnvtgllannssferdtlgsilgrsenpate 525 
CEN62609     472 glyeeq------------------gttmgapryedlpdafDLf-dIDDARnvtgllasqslgkgklgqlmvdy----eds 528 
Q5BEM5       474 gpsehvm----------------niekpntieskelpdafSLf-dINDAQhmtgllasdaslekaklgqllneadhdiek 536  Aspergillus ni...

Q4WSG8       544 pihphrknfgpemisavslsLAPLLSSQALLVDFSCLHLLFKE 586  Aspergillus fumigatus
XP_011124834 564 snqpttaryppplsvtfslsINPILQAQARLTNMACMRLLFKE 606 
GAD96537     548 qamtsdspfgppltsafhlsLAPIISAQARLIDFSCLHLLFKE 590 
CEO58992     541 dyvddasrfgpelssalylsLAPVISSQAQLIDFSYLHHLFKE 583 
CDM28698     544 edtnersylgpeltyglhlsLAPILSSQALLIDYSCLHLLFKE 586 
KGO68243     543 eninetsylgpeftsglhlsLAPILSSQALLIDYSCLHLLFKE 585 
EKV06197     376 dhtsegthlgpelmsglhlsIAPILSSQALLIDYSCLHLLFKE 418 
KKK20996     526 qaeeskmefspeltsslylsLAPLLSSQALLIDFSCLHLLFKE 568 
CEN62609     529 trercgtnfapevtsslhlsLAPLLSSQALLIDYSCLHLLFKE 571 
Q5BEM5       537 qsgkletnfapevtsslylsLAPLLSSQALLIDFSCLHLLFKE 579  Aspergillus nidulans
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