1LPP,1EA5,1CLE,1F6W,1THG,1QE3


Conserved Protein Domain Family
Esterase_lipase

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cd00312: Esterase_lipase 
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Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate.
Statistics
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PSSM-Id: 238191
Aligned: 23 rows
Threshold Bit Score: 440.23
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic triadsubstrate
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1LPP       18 TATLangDTITGLNAi------iNEAFLGIPFAEPPvgnLRFKDPVPYsgsldGQKFTSYGPSCMQQnpegtyeenlpka 91  Candida rugosa
1EA5_A      7 LVNTk-sGKVMGTRVpvl--sshISAFLGIPFAEPPvgnMRFRRPEPKkpwsgVWNASTYPNNCQQYvdeqfpgfsg--- 80  Pacific electric ray
1CLE_A      3 TAKLangDTITGLNAi------iNEAFLGIPFAEPPvgnLRFKDPVPYsgslnGQKFTSYGPSCMQQnpegtfeenlgkt 76  Candida cylindracea
1F6W_A      5 AVYTe-gGFVEGVNKklgllgdsVDIFKGIPFAAPT---KALENPQPHpgwqgTLKAKNFKKRCLQAtitqd-------- 72  human
116353     33 IIATk-nGKVRGMNLtvf--ggtVTAFLGIPYAQPPlgrLRFKKPQSLtkwsdIWNATKYANSCCQNidqsfpgfhg--- 106 human
119603     25 VVDTt-qGKVLGKYIslegfeqpVAVFLGVPFAKPPlgsLRFAPPQPAepwsfVKNATSYPPMCSQDagwakilsdmf-- 101 house mouse
730714     31 EVVTn-yGSVRGYQVkvnaaersVNVFLGLPFAKPPvgpLRFSEPQPPepwkgVRDAASYPPMCLQDkvlgqylsdai-- 107 Anas platyrhynchos
584715     20 LIHLhdgSPLFGEEVlsqt-gkpLTRFQGIPFAEPPvgnLRFKKPKPKqpwriPLNATTPPNSCIQSedtyfgdfyg--- 95  Caenorhabditis ele...
119578      7 VVDTv-kGKVLGKFVslegfaqpVAVFLGVPFAKPPlgsLRFAPPQPAeswshVKNTTSYPPMCSSDavsghmlself-- 83  rabbit
P18142     33 IVVTq-fGAIKGIVEd------tHRVFYGIPFAQPPvnqLRWENPIDLkpwenVRETLTQKSQCAQKcnlgpgv------ 99  Dictyostelium disc...
Feature 1                                                                                     
1LPP       92 aldlvmqskvfeavspsSEDCLTINVVRPpgtk-aganLPVMLWIFGGGFEVGGTstf--------ppAQMITKSiamgk 162 Candida rugosa
1EA5_A     81 -------semwnpnremSEDCLYLNIWVPspr---pksTTVMVWIYGGGFYSGSStldv------yngKYLAYTEe---- 140 Pacific electric ray
1CLE_A     77 aldlvmqskvfqavlpqSEDCLTINVVRPpgtk-aganLPVMLWIFGGGFEIGSPtif--------ppAQMVTKSvlmgk 147 Candida cylindracea
1F6W_A     73 -------------stygDEDCLYLNIWVPqgrkqvsrdLPVMIWIYGGAFLMGSGhganflnnylydgEEIATRGn---- 135 human
116353    107 -------semwnpntdlSEDCLYLNVWIPapk---pknATVLIWIYGGGFQTGTSslh-------vydGKFLARVe---- 165 human
119603    102 ------stekeilplkiSEDCLYLNIYSPadlt-kssqLPVMVWIHGGGLVIGGRsp----------yNGLALSAhe--- 161 house mouse
730714    108 ------tnrkekvrlqiSEDCLYLNVYTPvste-eqekLPVFVWIHGGGLVSGAAssy--------dgSALAAFDn---- 168 Anas platyrhynchos
584715     96 -------stmwnantklSEDCLYLNVYVPgkvd-pnkkLAVMVWVYGGGFWSGTAtld--------vyDGRILTVee--- 156 Caenorhabditis ele...
119578     84 ------tnrkeniplkfSEDCLYLNIYTPadlt-krgrLPVMVWIHGGGLMVGGAst----------yDGLALSAhe--- 143 rabbit
P18142    100 -----------cspigtSEDCLYLDVFTPkdat-pnskYPVIVYIPGGAFSVGSGsv---------plYDATKFAqs--- 155 Dictyostelium disc...
Feature 1                                                                  #                  
1LPP      163 pIIHVSVNYRVSSWGFLagdeikaegsaNAGLKDQRLGMQWVADNIAAFGGDPTKVTIFGESAGSMSVMCHILWndgdnt 242 Candida rugosa
1EA5_A    141 -VVLVSLSYRVGAFGFLalhgs-qeapgNVGLLDQRMALQWVHDNIQFFGGDPKTVTIFGESAGGASVGMHILSpgs--- 215 Pacific electric ray
1CLE_A    148 pIIHVAVNYRVASWGFLagddikaegsgNAGLKDQRLGMQWVADNIAGFGGDPSKVTIFGESAGSMSVLCHLIWndgdnt 227 Candida cylindracea
1F6W_A    136 -VIVVTFNYRVGPLGFLstgda--nlpgNYGLRDQHMAIAWVKRNIAAFGGDPDNITLFGESAGGASVSLQTLSpyn--- 209 human
116353    166 rVIVVSMNYRVGALGFLalpgn-peapgNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSpgs--- 241 human
119603    162 nVVVVTIQYRLGIWGLFstgde--hspgNWAHLDQLAALRWVQDNIANFGGNPDSVTIFGESSGGISVSVLVLSplg--- 236 house mouse
730714    169 -VVVVTIQYRLGIAGYFstgdk--hargNWGYLDQVAALQWIQENIIHFRGDPGSVTIFGESAGGVSVSALVLSpla--- 242 Anas platyrhynchos
584715    157 nVILVAMNYRVSIFGFLymnrp--eapgNMGMWDQLLAMKWVHKNIDLFGGDLSRITLFGESAGAASVSIHMLSpks--- 231 Caenorhabditis ele...
119578    144 nVVVVTIQYRLGIGGFGf----------NIDELFLVAVNRWVQDNIANFGGDPGSVTIFGESAGGQSVSILLLSplt--- 210 rabbit
P18142    156 sVIVVNINYRLGVLGFMgtdl----mhgNYGFLDQIKALEWVYNNIGSFGGNKEMITIWGESAGAFSVSAHLTFtys--- 228 Dictyostelium disc...
Feature 1                                                                                     
1LPP      243 ykgkpLFRAGIMQSGAMvpsdavd-giygneIFDLLASNAGCgsas-------dklACLRGVSSDTLEDAtnntpgfl-- 312 Candida rugosa
1EA5_A    216 ---rdLFRRAILQSGSPncpwasvsvaegrrRAVELGRNLNCnlnsd-----eeliHCLREKKPQELIDVewnvlpf--- 284 Pacific electric ray
1CLE_A    228 ykgkpLFRAGIMQSGAMvpsdpvd-gtygneIYDLFVSSAGCgsas-------dklACLRSASSDTLLDAtnntpgfl-- 297 Candida cylindracea
1F6W_A    210 ---kgLIRRAISQSGVAlspwvi--qknplfWAKKVAEKVGCpvgda-----armaQCLKVTDPRALTLAykvplagl-- 277 human
116353    242 ---hsLFTRAILQSGSFnapwavtslyearnRTLNLAKLTGCsrene-----teiiKCLRNKDPQEILLNeafvvpy--- 310 human
119603    237 ---kdLFHRAISESGVVintnvg--kkniqaVNEIIATLSQCndtss-----aamvQCLRQKTESELLEIsgklv----- 301 house mouse
730714    243 ---kgLFHKAISESGTAvrilft---eqpeeQAQRIAAAAGCeksss-----aalvECLREKTEAEMEQItlkm------ 305 Anas platyrhynchos
584715    232 ---apYFHRAIIQSGSAtspwaieprdvalaRAVILYNAMKCgnmslinpdydrilDCFQRADADALRENewapvr---- 304 Caenorhabditis ele...
119578    211 ---knLFHRAISESGVAllsslf--rkntksLAEKIAIEAGCkttts-----avmvHCLRQKTEEELMEVtlkmkfmald 280 rabbit
P18142    229 ---rqYFNAAISSSSPLtvglkd--kttargNANRFATNVGCnie---------dlTCLRGKSMDEILDAqekvgltfg- 293 Dictyostelium disc...
Feature 1                                                   #                                 
1LPP      313 ---aysslrlSYLPRPDGVNITDDMYALVregkyaNIPVIIGDQNDEGTFFgtsslnv--------------ttdaqaRE 375 Candida rugosa
1EA5_A    285 ----dsifrfSFVPVIDGEFFPTSLESMLnsgnfkKTQILLGVNKDEGSFFllygapgfskd------seskisredfMS 354 Pacific electric ray
1CLE_A    298 ---aysslrlSYLPRPDGKNITDDMYKLVrdgkyaSVPVIIGDQNDEGTVFglsslnv--------------ttnaqaRA 360 Candida cylindracea
1F6W_A    278 --eypmlhyvGFVPVIDGDFIPDDPINLYan--aaDIDYIAGTNNMDGHIFasidmpaink-------gnkkvteedfYK 346 human
116353    311 ----gtplsvNFGPTVDGDFLTDMPDILLelgqfkKTQILVGVNKDEGTAFlvygapgfskd------nnsiitrkefQE 380 human
119603    302 ------qyniSLSTMIDGVVLPKAPEEILaeksfnTVPYIVGFNKQEFGWIipmmlqnllpe-----gkmneetasllLR 370 house mouse
730714    306 -------ppmFISASLDGVFFPKSPRQLLsekvinAVPYIIGVNNCEFGWIlprmmkfpefte----glekdvarqvlQS 374 Anas platyrhynchos
584715    305 -----efgdfPWVPVVDGDFLLENAQTSLkqgnfkKTQLLAGSNRDESIYFltyqlpdifpvadfftktdfikdrqlwIK 379 Caenorhabditis ele...
119578    281 lvgdpkentaFLTTVIDGVLLPKAPAEIYeekkynMLPYMVGINQQEFGWIipmqmlgyplse----gkldqktatelLW 356 rabbit
P18142    294 --dkildaftIWSPVIDGDIIPMQPLTAVkegktyDVPTIIGNVKHEAIPFiysffqd--------------svgidyYR 357 Dictyostelium disc...
Feature 1                                                                                     
1LPP      376 YFKQSFVhas---daEIDTLMTAYpgditqgspfdtgilnaltpqfkRISAVLGDLGFTLAr-RYFLNHYtg----gtKY 447 Candida rugosa
1EA5_A    355 GVKLSVPhan---dlGLDAVTLQYtdwmddn---------ngiknrdGLDDIVGDHNVICPl-MHFVNKYtkf--gngTY 419 Pacific electric ray
1CLE_A    361 YFKQSFIhas---daEIDTLMAAYpqditqgspfdtgifnaitpqfkRISAVLGDLAFIHAr-RYFLNHFqg----gtKY 432 Candida cylindracea
1F6W_A    347 LVSEFTItkg---lrGAKTTFDVYteswaqdp--------sqenkkkTVVDFETDVLFLVPt-EIALAQHranaksakTY 414 human
116353    381 GLKIFFPgvs---efGKESILFHYtdwvddq---------rpenyreALGDVVGDYNFICPa-LEFTKKFsew--gnnAF 445 human
119603    371 RFHSELNis----esMIPAVIEQYlrgvdd-----------pakkseLILDMFGDIFFGIP--AVLLSRSlrda-gvsTY 432 house mouse
730714    375 TLALSFKgap---sdIVDLVYNEYigvaen-----------raqvrdGLLDSIADPLFVFSa-VEVARHHrda--gnpVY 437 Anas platyrhynchos
584715    380 GVKDLLPrqilkcqlTLAAVLHEYepqdlpv---------tprdwinAMDKMLGDYHFTCSvnEMALAHTkhg---gdTY 447 Caenorhabditis ele...
119578    357 KSYPIVNvs----keLTPVATEKYlggtdd-----------pvkkkdLFLDMLADLLFGVPs-VNVARHHrda--gapTY 418 rabbit
P18142    358 VLVAIVFp------lNAMKILPLYpaaprg------------qdsrpILSELITDYLFRCPd-RYHTVTNakkl-sspTY 417 Dictyostelium disc...
Feature 1                           #                                                         
1LPP      448 SFLSKqlsgl------pvlgTFHSNDIVFQDyllgs------------gsliyNNAFIAFATDLDPNtag----llvKWP 505 Candida rugosa
1EA5_A    420 LYFFNhrasnlv--wpewmgVIHGYEIEFVFglplvkelnyt-aeeealsrriMHYWATFAKTGNPNephs---qesKWP 493 Pacific electric ray
1CLE_A    433 SFLSKqlsgl------pimgTFHANDIVWQDyllgs------------gsviyNNAFIAFATDLDPNtag----llvNWP 490 Candida cylindracea
1F6W_A    415 AYLFShpsrmpv--ypkwvgADHADDIQYVFgkpfatptgyr-pqdrtvskamIAYWTNFAKTGDPNmgds--avptHWE 489 human
116353    446 FYYFEhrssklp--wpewmgVMHGYEIEFVFglplerrdnyt-kaeeilsrsiVKRWANFAKYGNPNetqn---nstSWP 519 human
119603    433 MYEFRyrpsfvsdkrpqtveGDHGDEIFFVFgapllkegas--eeetnlskmvMKFWANFARNGNPNgeg-----lpHWP 505 house mouse
730714    438 FYEFQhrpssaagvvpefvkADHADEIAFVFgkpflagnat--eeeaklsrtvMKYWTNFARNGNPNgeg-----lvHWP 510 Anas platyrhynchos
584715    448 YYYFThrasqqt--wpewmgVLHGYEINFIFgeplnqkrfnytdeerelsnrfMRYWANFAKTGDPNknedgsftqdVWP 525 Caenorhabditis ele...
119578    419 MYEYRyrpsfssdmrpktviGDHGDEIFSVLgapflkegat--eeeiklskmvMKYWANFARNGNPNgeg-----lpQWP 491 rabbit
P18142    418 HYHYVhvkstghsldacddkVCHGTELSLFFnsyelmgerld-ndekelaidiNNYIVNFATTHNPNtgls---vpvQWR 493 Dictyostelium disc...
Feature 1                               
1LPP      506 EYtsssqsgnNLMMIna--lgLYTGK 529 Candida rugosa
1EA5_A    494 LFttk---eqKFIDLnt--epMKVHQ 514 Pacific electric ray
1CLE_A    491 KYtsssqsgnNLMMIna--lgLYTGK 514 Candida cylindracea
1F6W_A    490 PYtte---nsGYLEItkkmgsSSMKR 512 human
116353    520 VFkst---eqKYLTLnt--esTRIMT 540 human
119603    506 EYde----qeGYLQIga---tTQQAQ 524 house mouse
730714    511 QYdm----deRYLEIdl---tQKAAK 529 Anas platyrhynchos
584715    526 KYnsv---smEYMNMtvessyPSMKR 548 Caenorhabditis elegans
119578    492 AYdy----keGYLQIga---tTQAAQ 510 rabbit
P18142    494 QVtst---qnSTLILet---tIETKD 513 Dictyostelium discoideum

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