1OCU,1OCS


Conserved Protein Domain Family
PX_Grd19
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cd07295: PX_Grd19 
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The phosphoinositide binding Phox Homology domain of fungal Grd19
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.
Links
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Statistics
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PSSM-Id: 132828
Aligned: 5 rows
Threshold Bit Score: 194.639
Created: 2-Mar-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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phosphoinosit..
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:1OCU_A; Saccharomyces cerevisiae SNX3 (Grd19p) PX domain binds Phosphatidylinositol-3-Phosphate; defined at 3.5A contacts.
    - View structure with Cn3D
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                   # #  #                         ## 
1OCU_A     35 FLEIEVHNPKTHIpngmdskgMFTDYEIICRTNLPSFHKRVSKVRRRYSDFEFFRKXLIKeismlnhpKVMVPHLPGKIl 114 baker's yeast
1OCS_A     35 FLEIEVHNPKTHIpngmdskgMFTDYEIICRTNLPSFHKRVSKVRRRYSDFEFFRKXLIKeismlnhpKVMVPHLPGKIl 114 baker's yeast
XP_965545  22 FLEIEVRNPRTHGig----rhMYTDYEIVCRTNIPAFKLRQSTVRRRYSDFEYFRDILERes-----aRVTIPPLPGKVf 92  Neurospora crassa ...
NP_596480  24 ILEIDVINPQTHGig----rnMFTTYEIVCRTNMPYFRLHNSSVRRRYSEFEKFHDMLERes-----gRVSIPPLPGKIf 94  fission yeast
Q4P1V3      8 FLEVEVRSPLTHGvg----rkMYTDYEIVTRTNIPAFKLRYSSVRRRYSDFEYFRDILERes-----tRVNIPPLPGKVf 78  Ustilago maydis

Feature 1        #        #                                 
1OCU_A    115 lsnRFSNEVIEERRQGLNTWMQSVAGHPLLQsGSKVLVRFIEAEKF 160 baker's yeast
1OCS_A    115 lsnRFSNEVIEERRQGLNTWMQSVAGHPLLQsGSKVLVRFIEAEKF 160 baker's yeast
XP_965545  93 t-nRFSDDVIEGRRAGLEKFLKIVVGHPLLQtGSKVLAAFVQDPNW 137 Neurospora crassa OR74A
NP_596480  95 t-qRFRDDVIEERRQGLENFLRLVAGHPLIQtHSRVLSSFLQSPEF 139 fission yeast
Q4P1V3     79 t-nRFTDEVIEARREGLERFLQVVAGHPLLQtGSKVMAAFLQDSGW 123 Ustilago maydis

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