GEO DataSets

(Submitter supplied) Transcript profiling was performed by Affymetrix microarray analysis and SAGE to characterize changes in gene expression in the bovine mammary gland in response to 4× versus 2× daily milking during the first week of lactation. These changes in gene expression may contribute to the increased milk production observed in response to increased milking frequency. Our results indicate that increased milking frequency alters expression of genes in the mammary gland related to increased extracellular matrix remodeling, neovascularization, metabolism, cell proliferation and apoptosis. more...

Organism:

Bos taurus

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL2112 GPL223

9 Samples

Download data: CEL

(Submitter supplied) The goal of this study was to study the gene expression changes that occurred in BL3 cells that were infected with type 2 bovine viral diarrhea virus (BVDV2). What happens within the cell and how the cell responds to the virus is completely unknown. Keywords: gene expression changes, BVDV2-infected cells, acute infection

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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(Submitter supplied) Papillomatous digital dermatitis (PDD) is a polymicrobial infection in soft tissue adjacent to the hoof and is the leading cause of lameness in dairy cattle. Treponema phagedenis-like (TPL) spirochetes are a constant feature of PDD lesions and are localized deep in infected tissue. Host-cell response mechanisms to TPL bacteria are poorly understood. To assess how bovine macrophages respond to cellular constituents of TPL spirochetes, changes in transcription were analyzed using serial analysis of gene expression (SAGE) and real time RT-PCR. more...

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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(Submitter supplied) Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

3 Samples

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(Submitter supplied) Gene expression profiling of pooled lymphocyte populations from clinically normal cattle. The T lymphocyte populations were purified from buffy coat and the immature B cells were prepared from isolated Peyer's patch follicles. Keywords: other

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

4 Samples

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(Submitter supplied) Analysis of gene expression induced in Madin-Darby bovine kidney cells (MDBK) infected with Bovine viral diarrhoea virus BVDV2 strain 1373. Keywords: other

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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(Submitter supplied) SAGE libraries were prepared from mRNA of cultured bovine glomerular and aortic endothelial cells. Cells both cell lines were positive for acetylated LDL uptake and vWF expression, and were proven to be free of non-endothelial cells. Cultures were studied at passage 9-10. The cells were confluent, cultured in parallel, under identical conditions. Cells were plated on gelatin coated cell culture plastic in RPMI 1640 medium containing 10% FBS and streptomycin/penicillin. more...

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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