GEO DataSets

(Submitter supplied) We found piRNAs with different lengths represented the predominant small RNA species in oocytes from the 12 explored species, except mouse. We found endo-siRNAs resulted from the truncated Dicer isoform were mouse-specific, and os-piRNAs associating with PIWIL3 in human oocytes are widespread in mammals and are typically with low levels of the 2’-3’-O-methylation. The sequences of many highly expressed piRNA clusters are fast-evolving compared with their syntenic genomic locations, and the TE families distributing in the conserved piRNA clusters are various between species.

Organism:

Danio rerio; Homo sapiens; Canis lupus familiaris; Sus scrofa domesticus; Cavia porcellus; Macaca fascicularis; Oryctolagus cuniculus; Cricetulus griseus; Mesocricetus auratus; Mus musculus; Capra hircus; Rattus norvegicus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

12 related Platforms

138 Samples

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(Submitter supplied) Hypertrophic scar (HS) considerably affects the appearance and causes tissue dysfunction in patients. Here we show a separating microneedle (MN) consisting of photo-crosslinked GelMA and 5-FuA-Pep-MA prodrug in response to high reactive oxygen species (ROS) levels and overexpression of matrix metalloproteinases (MMPs) in the HS pathological microenvironment. RNA sequencing analyses confirm that drug-loaded MNs could reverse skin fibrosis through down-regulation of BCL-2-associated death promoter (BAD), insulin-like growth factor 1 receptor (IGF1R) pathways, simultaneously regulate inflammatory response and keratinocyte differentiation via up-regulation of toll-like receptors (TOLL), interleukin-1 receptor (IL1R) and keratinocyte pathways.

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

12 Samples

Download data: TXT

(Submitter supplied) Translation termination is an essential cellular process that is also of therapeutic interest for diseases that manifest from premature stop codons. In eukaryotes, translation termination requires eRF1, which recognizes stop codons, catalyzes the release of nascent proteins fom ribosomes, and facilitates ribosome recycling. The small molecule SRI-41315 triggers eRF1 degradation and enhances translational readthrough of premature stop codons. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

12 Samples

Download data: BEDGRAPH

(Submitter supplied) This study aimed to investigate the effects of long-term pollution from different wavelengths of light on the corneal epithelium (CE) and identify potential biomarkers. Rabbits were exposed to red, green, blue, white, and environmental light for 6 weeks. The CE was assessed using various techniques such as fluorescein sodium staining, transcriptome sequencing, electron microscopy, and molecular assays. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

12 Samples

Download data: TXT

(Submitter supplied) To investigate the effect of treprostinil pulmonary development in a rabbit model of congenital diaphragmatic hernia.

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

8 Samples

Download data: CSV

(Submitter supplied) In this study, the methods to isolate and identify extracellular vesicles (EVs) including exosomes, from the seminal plasma (SP) of 3 fertile (F) and subfertile (S) bucks have been developed. Additionally, we investigated whether specific miRNA abundance differences between F and SF bucks could serve as fertility biomarkers. Ultracentrifugation and size exclusion chromatography analysis have made it possible to isolate different SP-EVs concentrations (8.53x10^11 ± 1.04x10^11 and 1.84x10^12 ± 1.75x10^11 particles/ml of SP; p=0,008), with a similar average size (143.9 ± 11.9 and 115.5 ± 2.4 nm; p=0.7422) in F and S males, respectively. more...

Organism:

Oryctolagus cuniculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26786

6 Samples

Download data: XLSX

(Submitter supplied) Proliferative vitreoretinopathy (PVR) is the most common cause of failure of retinal reattachment surgery and the molecular changes leading to this aberrant wound healing process is currently unknown. We aimed to study PVR pathogenesis using single-cell transcriptomics to dissect cellular heterogeneity in a rabbit PVR model. PVR was induced unilaterally in Dutch Belted rabbits. At different timepoints following PVR induction, retinas were dissociated into either cells or nuclei suspension and processed for single-cell or single-nucleus RNA sequencing (scRNA-seq or snRNA-seq). more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

10 Samples

Download data: CSV

(Submitter supplied) Conditions affecting skeletal muscle, such as chronic rotator cuff tears, low back pain, dystrophies, and many others often share changes in muscle phenotype: intramuscular adipose and fibrotic tissue increase while contractile tissue is lost. The underlying changes in cell populations and cell ratios observed with these phenotypic changes complicate the interpretation of tissue-level transcriptional data. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

4 Samples

Download data: JPG, JSON, MTX, TSV

(Submitter supplied) We developed a thermogelling polymer with intrinsic anti-scarring properties on retinal cells in-vitro. To study its mechanism of action in-vivo, this thermogelling polymer was injected into the rabbit eye as a vitreous substitute and the retinal tissues were harvested for RNA-sequencing to understand transcriptomic changes induced by the polymer.

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

18 Samples

Download data: CSV

(Submitter supplied) We report RNA-seq data obtained on dissected aorta and four heart valves from rabbits fed with chow diet (n=7), cholesterol-rich diet (n=6) or lard-rich diet (n=7). Rabbits that received cardiac irradiation combined with the cholesterol-rich diet were also included (n=7).

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

134 Samples

Download data: TXT

(Submitter supplied) Brown adipose tissue (BAT) in rabbits undergoes rapid involution, i.e. transforming to white adipose tissue (WAT), similarly to what happens in humans. We aim to profile the transcriptomic changes of total BAT and the stromal vascular fraction (SVF) that contains adipocyte progenitors at the global and single-cell levels.

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28431 GPL26786

39 Samples

Download data: TXT, XLSX

(Submitter supplied) We investigated the roles of IL-6 family members during the wound healing process after trabeculectomy (TL). At the surgical site, the expression levels of genes encoding IL-6, oncostatin M, their receptors, and collagen I were elevated at 3 hours after TL, whereas the levels of genes encoding TGF-β, α-SMA, type IV collagen, and fibronectin were elevated at 3 days after TL.

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus; Macaca mulatta; Homo sapiens; Canis lupus familiaris; Cavia porcellus; Sus scrofa; Mus musculus; Felis catus; Ovis aries; Oryctolagus cuniculus; Rattus norvegicus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

19 related Platforms

553 Samples

Download data: HIC, TXT

(Submitter supplied) Comprehensive transcriptomic survey of the pig (Sus scrofa) may lead to a better understanding of mechanisms of tissue specialization that underlie economic traits of this species and accelerate its use as a biomedical model. Here, we characterized four distinct transcript types (lncRNAs, TUCPs, miRNAs and circRNAs) in 31 adult pig tissues and two cell lines, together with protein-coding genes. We dissected their distinct structural and transcriptional features and uncovered transcriptome variability as related to tissue physiology. more...

Organism:

Felis catus; Ovis aries; Rattus norvegicus; Sus scrofa; Oryctolagus cuniculus; Canis lupus familiaris

Type:

Genome binding/occupancy profiling by high throughput sequencing

6 related Platforms

16 Samples

Download data: HIC, TXT

(Submitter supplied) The purpose of this study is to investigate if acute mucosal dehydration as a funcition of low relative humidty environment impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered mucosal hydration state in response to low ambient humidity on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

12 Samples

Download data: TSV

(Submitter supplied) We developed a microbial catalytic concept and strategy to prepare calcium carbonate with micro/nanostructures on the surface of bioceramics to improve bone-forming bioactivity. It involves three processes: bacterial adhesion on biomaterials, production of carbonate assisted with bacteria, nucleation and growth of calcium carbonate nano-crystals on the surface of bioceramics. The microbially catalyzed biominerals exhibited relatively uniform micro/nanostructures on both 2D and 3D CaSiO3 bioceramics. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

9 Samples

Download data: TXT

(Submitter supplied) The cornea is the most innervated tissue in the human body. Myelinated axons upon inserting into the peripheral corneal stroma lose their myelin sheaths and continue into the central cornea wrapped by only nonmyelinating corneal SCs (nm-cSCs). This anatomical organization is believed to be important for central vision. Here we employed single-cell RNA sequencing (scRNA-seq), microscopy, and transgenics to characterize these nm-cSCs of the central cornea. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

1 Sample

Download data: CSV

(Submitter supplied) The purpose of this study is to investigate if acute systemic dehydration impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered hydration state in response to furosemide on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

10 Samples

Download data: TXT

(Submitter supplied) Mutations in the metabolic enzyme fumarylacetoacetate hydrolase (FAH) cause hereditary tyrosinemia type I (HT1) in human. HT1 patients present acute and irreversible liver and kidney damage during infancy. CRISPR/Cas9-medated precise correction of disease-causing mutations in the liver of infant may provide a promising approach for the treatment of monogenetic liver metabolic disorders. However, to date, all previous precise gene therapy studies were conducted in adult HT1 rodent models (mice and rats), which are not able to recover irreversible pathological changes and result in less treatment effectiveness. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26786

36 Samples

Download data: CSV

(Submitter supplied) Mutations in the metabolic enzyme fumarylacetoacetate hydrolase (FAH) cause hereditary tyrosinemia type I (HT1) in human. HT1 patients present acute and irreversible liver and kidney damage during infancy. CRISPR/Cas9-medated precise correction of disease-causing mutations in the liver of infant may provide a promising approach for the treatment of monogenetic liver metabolic disorders. However, to date, all previous precise gene therapy studies were conducted in adult HT1 rodent models (mice and rats), which are not able to recover irreversible pathological changes and result in less treatment effectiveness. more...

Organism:

Oryctolagus cuniculus

Type:

Genome variation profiling by high throughput sequencing

Platform:

GPL26786

60 Samples

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