GEO DataSets

(Submitter supplied) To understand the mechanism by which miR-335 acts anti proliferative factor, we profiled mRNA expression using microarray platform ( codelink array). Embryonic day 12.5 neural cortical epithelium was isolated and grown as neurospheres in defined medium. Cultures were transfected with 2'-O-methyl (2'OMe)-base modified miRNA-335 antisense (2'OMe-335) and control (2' O Me-EGFP). To verify the suppression of miR-335 after the 2'-OMe treatment, we used real time RT-PCR which confirmed the down regulation of miR-335 up to ~4.5 cycles (~24 fold). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TXT

(Submitter supplied) Background: The COP9 signalosome (CSN) consisting of 8 protein subunits (CSN1 through CSN8) is a deneddylase for Cullin-RING ligases (CRLs), essential to the functioning of the ubiquitin-proteasome system. CSN8 is encoded by the Cops8 gene. We have shown that CSN8 plays a key role in the regulation of cardiac protein quality control (PQC), and cardiomyocyte-restricted Cops8 knockout (CSN8-CKO) in perinatal and adult mice causes massive cardiomyocyte necrosis and acute heart failure in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

18 Samples

Download data: TXT

(Submitter supplied) Xeroderma pigmentosum type A (XP-A) is a hereditary disease characterized by early onset of skin cancers development by ultraviolet (UV) exposure. Although etiology of susceptible to skin tumor of XP-A is well investigated as a repair deficiency by UV-induced DNA damage, the mechanism of unusual strong and prolonged skin inflammation caused by UV in XP-A and whether UV-induced inflammatory response relates to skin tumor-prone phenotype remains to be elucidated. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

5 Samples

Download data: TXT

(Submitter supplied) Gene expression profiling was used to identify genes that display radiation-induced transcriptional change over tumor histopathology. Keywords: mouse squamous cell carcinoma, fibrosarcoma and mammary carcinoma, gamma-irradiation, resected sample, transplanted tissues

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

121 Samples

Download data

(Submitter supplied) Mouse macrophages J774A.1 were pre-treated with the anti-inflammatory lipid eicosapenaenoic acid (EPA) or the pro-inflammatory lipid ceramide (Cer) for 3h. Macrophages were then infected with Mycobacterium smegmatis for 1h, and total RNA was collected. In a parallel experiment, infected macrophages were infected for 1h, 4h and 24h. At these time points, macrophages were lysed, bacteria was collected and quantified by the Colony Forming Units (CFU) Assay. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

24 Samples

Download data: TXT

(Submitter supplied) The ubiquitin-proteasome and autophagy-lysosome pathways are the two major routes for protein and organelle clearance. In skeletal muscle, both systems’ excessive activation induces severe muscle loss. Although altered proteasomal function has been observed in various myopathies, the specific role of proteasomal activity in skeletal muscle has not been determined by loss-of-function approaches. Here, we report that muscle-specific deletion of a crucial proteasomal gene, Rpt3, resulted in profound muscle atrophy and decrease in force. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TXT

(Submitter supplied) Codelink® Whole Mouse Genome Bioarrays (GE/Amersham) of one sample of WT, D6, D54, WT/D6, WT/D54 and MIGR NIH3T3 cells, cultivated in the four different conditions (SCF+, SCF- and imatinib 1 or 5 µM), were used for the analysis of differential gene expression. Our aim was to choose the best candidate genes for low density array analysis. We first selected genes differentially expressed between WT and hemizygous samples grown in the SCF+ condition, whose fold change was superior or equal to 2 and whose expression for at least one of the sample was greater than 1.3 (commonly admitted threshold for low density arrays). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

24 Samples

Download data: TXT

(Submitter supplied) These mice have been genetically modified so that the mouse PLN gene has been replace with the human PLN gene. Phopspholamban (PLN), the reversible inhibitor of the sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA2a), is a key regulator of myocyte Ca2+-cycling with a significant role in heart failure. We previously showed that the single amino acid difference between human and mouse PLN results in increased inhibition of Ca2+-cycling, cardiac remodeling and attenuated stress responses, in transgenic mice expressing the human PLN in the null background (hPLN). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

12 Samples

Download data: TXT

(Submitter supplied) Helicobacter pylori (H. pylori) infection and excessive salt intake are known as important risk factors for stomach cancer in humans. However, interactions of these two factors with gene expression profiles during gastric carcinogenesis remain unclear. In this study, we established a mouse gastric tumor model combining a chemical carcinogen, H. pylori infection and a high-salt diet. The tumor incidence and multiplicity in N-methyl-N-nitrosourea-treated mice were significantly increased by combination of H. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

4 Samples

Download data: TXT

(Submitter supplied) Background: Spina bifida is one of the most common and life threatening human congenital defects. Despite considerable effort and investigations, the causes and mechanisms underlying this malformation remain poorly characterized. In order to better understand pathogenesis of this abnormality, we conducted a microarray study to compare gene expression profiles between two mouse models, CLX-Splotch and Fkbp8Gt(neo), that both have spina bifida. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

12 Samples

Download data: TXT

(Submitter supplied) Three wildtype and three nullizygous embryonic posterior neural tube tissues were collected at embryonic day 9.5 and the gene expression patterns were profiled on CodeLink Mouse Whole Genome Bioarrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TXT

(Submitter supplied) Three wildtype and three nullizygous embryonic posterior neural tube tissues were collected at embryonic day 9.5 and the gene expression patterns were profiled on CodeLink Mouse Whole Genome Bioarrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TXT

(Submitter supplied) Embryonic stem cells studies have generated a great interest due to their ability to form a wide variety of matured cells. However, there remains a poor understanding of mechanisms regulating the cell state of ESCs and of the genes they express during early differentiation. Gene expression analysis may be a valuable tool to elucidate either the molecular pathways involved in self-renewal and pluripotency or early differentiation and to identify potential molecular therapy targets. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL2894 GPL2897

18 Samples

Download data: TXT

(Submitter supplied) The ING familiy of tumor suppressor proteins control several cellular functions relevant to anti-tumor protection, like cell-cycle control, apoptosis, senescence or migration. ING proteins are functionally linked to the p53 pathway, and they participate in transcriptional control via the recognition of histone marks and recruitment of protein complexes with chromatin-modifying activity to specific promoters. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TXT

(Submitter supplied) Induced overexpression of Pdx1 in activin-induced endoderm population resulted in the upregulation of pancreas-related genes such as insulin 1 and 2 at day 20. To enhance the developmental progression from the pancreatic bud to the formation of the endocrine lineages, we next expressed neurogenin3 (Ngn3) together with Pdx-1. Induced overexpression of Pdx1 together with Ngn3 dramatically increased Insulin 1 mRNA by day 9 differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

10 Samples

Download data: TXT

(Submitter supplied) Analysis of the effects of sleep deprivation, recovery sleep, and three time-of-day controls on seven brain regions laser microdissected from mouse brain. The regions include the locus coeruleus, suprachiasmatic nucleus, hypocretin area, tuberomammillary nucleus, orbital cortex, posteromedial cortical amygdala, and entorhinal cortex.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

140 Samples

Download data: TXT

(Submitter supplied) The ApoE -/- mice model of abdominal aortic aneurysm (AAA) involves introducing Angiotensin II subcutaneously to 14 week old male mice for 4 weeks by osmotic pump. A significant number of mice will develop aneurysm-like dilations in the suprarenal section of the abdominal aorta (SRA) that have a number of similarities to the human condition and make this a useful model of AAA. The mouse infrarenal aorta is very resistant to aneurysm formation while in humans AAA predominately occurs in the infrarenal section of the aorta (IRA). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

10 Samples

Download data

(Submitter supplied) In isolated heart preparations, we observed that previous treatment with quinazoline DMA evoked both acute and late phases of cardioprotection. In this study, we adopted whole genome microarrays approach to investigate cardiac transcriptional responses induced in hearts from DMA treated mice in comparison to vehicle-only treatment. Deckmann AC, Rocco SA, Marin RM, Rolim L, Carazzolle MF, Barau JG, Pereira GAG and Franchini KG. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

12 Samples

Download data

(Submitter supplied) Mice possessing the lethal yellow (LY) agouti mutation (C57BL/6J Ay/a) exhibit adult-onset obesity, altered metabolic regulation, and early reproductive senescence. The present study was designed to test the hypothesis that aging obese mice possess differences in expression of ovarian genes involved in reproductive function relative to age-matched lean mice. To synchronize ovarian state and exclude gonadotropin-mediated effects, 90- and 180-day old diestrous LY and lean black (C57BL/6J a/a) mice were suppressed with GnRH antagonist (Antide®) for five days, then injected with 5 IU equine chorionic gonadotropin (eCG). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

12 Samples

Download data

(Submitter supplied) This study focuses on the impact of developmental Pb exposure on splenic gene expression. Pb exposure from gestational day 8 (gd8) to post-natal day 21 (pnd21) was used to obtain insight into how Pb induces anemia and negatively influences the immune response Keywords: exposure analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data: TIFF