GEO DataSets

(Submitter supplied) Congestive heart failure was done by artificial myocardial infarction. After extracting total RNA from control and infarcted ventricles with Triazol, messenger RNA was isolated with Qiagen mRNA isolation kit. About 1.5ug poly A+ RNA was taken for probe preparation. Probe was labeled with Alexa Fluor 546 and 657. Labeled probes were hybridized with Qiagen rat unigene oligo library. After 2 low and 1 high stringency washes Prolong Antifade was added to the slides to prevent bleaching effect. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL333

6 Samples

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(Submitter supplied) Rat ventricular cardiomyocytes were prepared from 18 day old fetus. Approximately 6 million cells were plated on 100 mm culture plate. The serum used in the medium was depleted from thyroid hormone by passing through anion exchange resin column. After 48 hours of growth with 70% confluency, cells were exposed to either T3, DITPA or CGS for another 48 hours before harvesting. Final concentrations of T3, DITPA and CGS in the medium were 6nM, 218nM and 1nM respectively. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS468 GDS469

Platform:

GPL333

10 Samples

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Analysis of effect of 48 hours exposure to thyroid hormone triiodothyronine (T3) and structural analogues DITPA and CGS on primary culture cardiomyocytes prepared from 18 day old fetus. Thyroid hormone may be useful in treatment of heart failure.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, log ratio, 3 agent sets

Platform:

GPL333

Series:

GSE602

3 Samples

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Analysis of effect of 48 hours exposure to thyroid hormone triiodothyronine (T3) and structural analogues DITPA and CGS on primary culture cardiomyocytes prepared from 18 day old fetus. Thyroid hormone may be useful in treatment of heart failure.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, log ratio, 3 agent sets

Platform:

GPL333

Series:

GSE602

3 Samples

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(Submitter supplied) Rat genome oligo set comprises 4273 UniGene clones Keywords = Unigene Oligo Nucleotide Array

Organism:

Rattus norvegicus

2 DataSets

2 Series

16 Samples

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