GEO DataSets

(Submitter supplied) Aldosterone-producing adenomas (APA) are heterogeneous. The objectives of this study were to compare the transcriptional profiles in APA and normal adjacent adrenal gland (AAG). This was done through the Illumina beadchip analysis of RNA from eight paired APA-AAG. Other tissues (phaeochromocytoma, cushing, and hyperplastic adrenals) were included as controls.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

24 Samples

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(Submitter supplied) Trascriptional profiling of human AML cells after SirT activity modulation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

12 Samples

Download data: TXT

(Submitter supplied) Myeloid dendritic cells (mDC) were isolated from antiretroviral therapy (ARV)-treated and untreated people living with HIV (PLWH), and from HIV uninfected Individuals. The results indicate that mDC are altered in genes expression from PLWH. Some RNA transcriptional changes are not completely restored by ARV. This provides more data on myeloid cells, an understudied cell type, and alterations in PLWH.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL10558 GPL6883

34 Samples

Download data: TXT

(Submitter supplied) Recent studies of cortical pathology in secondary progressive multiple sclerosis have shown that a more severe clinical course and the presence of extended subpial grey matter lesions with significant neuronal/glial loss and microglial activation are associated with meningeal inflammation, including the presence of lymphoid-like structures in the subarachnoid space in a proportion of cases. To investigate the molecular consequences of pro-inflammatory and cytotoxic molecules diffusing from the meninges into the underlying grey matter, we carried out gene expression profiling analysis of the motor cortex from 20 post-mortem multiple sclerosis brains with and without substantial meningeal inflammation and 10 non-neurological controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

50 Samples

Download data: TXT

(Submitter supplied) Ex vivo red blood cell (RBC) differentiation from human induced pluripotent stem cells (hiPSCs) offers great potential for developmental studies and innovative therapies. However, ex vivo erythropoiesis from hiPSCs is currently limited by its complexity and the absence of a physiological niche, which may impair cell growth and lineage-specific differentiation. We describe a simplified culture system for RBC generation over 6 weeks from hiPSCs using 1) low cytokine support (SCF, EPO, and IL-3) and 2) maintenance of a three-dimensional organization during hematopoietic specification. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL10558 GPL6883

3 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL23159 GPL6883 GPL18573

35 Samples

Download data: BEDGRAPH, CEL

(Submitter supplied) CD34+ progenitor cells were cultured for 0 or 21 days in StemPro medium + supplement + IL-3 at 5 ng/ml Basophils, eosinophils and plasmacytoid dendritic cells were purified to near homogeneity and lysed for miRNA and mRNA. Basophils were purified by negative selection (StemSep Abs, miltenyi columns), eosinophils by StemSep Abs and pDC by positive selection after removing monocytes. The method starting with a 2-step Percoll gradient that put eosinophils in the pellet, basophils in the middle layer and pDC in the upper layer.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

21 Samples

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(Submitter supplied) Cell-cell communication between keratinocytes and fibroblasts is essential for skin homeostasis, regulation and regeneration. How extracellular cytokine signaling, intra-cellular protein signaling and transcriptional regulation initiate and maintain this communication is still largely unknown. Here, we study in vitro the secretome and transcriptome dynamics that establish double paracrine cell-cell communication between primary human keratinocytes and fibroblasts. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

16 Samples

Download data: TXT

(Submitter supplied) Rationale: Serial measurements of genome-wide transcriptional changes in alveolar macrophages (AMs) in patients with acute respiratory distress syndrome (ARDS) could identify dynamic biologic processes that are associated with clinical outcomes. Objectives: To identify associations between AM transcriptional programs and the composite endpoint of ventilator-free days (VFDs) over the course of ARDS. Methods: We performed unbiased genome-wide transcriptional profiling of AMs purified from bronchoalveolar lavage fluid collected from patients with ARDS. Cells were obtained at baseline (Day 1), Day 4, and Day 8 after ARDS onset. We assessed pathway enrichment in subjects with VFDs > 0 (VFD-Extubated/Alive) versus VFDs = 0 (VFD-Intubated/Dead) at each time point. Measurements and Main Results: We found highly divergent AM transcriptional patterns at all time points between ARDS patients based on their VFD status (FDR < 0.05). “M1-like” and pro-inflammatory gene sets such as IL-6-JAK-STAT signaling were significantly enriched in AMs isolated on Day 1 in VFD-Extubated/Alive versus VFD-Intubated/Dead subjects. In contrast, many of these same gene sets were associated with the VFD-Intubated/Dead subjects on Day 8. In patients who had samples from each time point, we identified multiple AM gene clusters whose temporal expression patterns were associated with VFD status. The relationship between AM expression profiles and VFDs was distinct in subjects with Direct (pulmonary) versus Indirect (extrapulmonary) ARDS. Conclusion: Clinically meaningful outcomes over the course of ARDS are associated with highly distinct AM transcriptional programs. Our findings suggest that interventions targeting the alveolar immune response should be tested within strictly defined time periods.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

68 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL8490 GPL6883

34 Samples

Download data: IDAT

(Submitter supplied) To identify genes whose products are involved in the formation of a pathological phenotype of varicose veins, we attempted to analyze the transcriptome of their constituent cells and compare it to the transcriptome of normal veins from the same patient. We identified a number of genes of extracellular matrix remodeling and cell-cell communication significantly changing gene expression status in disease condition.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

14 Samples

Download data: IDAT, TXT

(Submitter supplied) Non-toxic cocentrations of VCC induces upregulation of inflammatory genes in T84 monolayer cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

2 Samples

Download data: TXT, XLS

(Submitter supplied) Induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) can be differentiated into hepatocyte-like cells (HLCs) and thus provide a defined and renewable source for toxicology and regenerative medicine. Critical comparison of patient-specific iPSCs to the golden standard—hESCs will provide an assessment of their strengths and weaknesses. This dataset consists of data at the transcriptional level during the differentiation process of hESCs into HLCs including the definite endoderm (DE) and hepatic endoderm (HE) stages. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6883 GPL10558

52 Samples

Download data

(Submitter supplied) Analysis of role of small intestinal intraepithelial lymphocytes (IELs) in the immunopathology of celiac disease

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

8 Samples

Download data: TXT

(Submitter supplied) Analysis of role of small intestinal epithelial cells (IECs) in the immunopathology of celiac disease

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

8 Samples

Download data: TXT

(Submitter supplied) A549 lung cancer cells were not infected (NI), transfected by plasmids containing shGLP (sequence targeting GLP gene) or shNS (non-specific sequence). These cells were treated with dex to identify subsets of genes regulated by dex (hormone) and GLP (EHMT1). NI and shNS data were submitted on the GSE35962.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

8 Samples

Download data: TXT

(Submitter supplied) The current standard-of-care for advanced liver cancer is limited. Therefore, there is an urgent need for development of novel molecular targeted therapy. Increase of WEE1 kinase activity through an epigenetic regulation plays an important role in the development of hepatocellular carcinoma (HCC). Here we demonstrated that WEE1 siRNA silencing caused inhibition of HCC cell growth through blockade of cell cycle progression and induction of apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

8 Samples

Download data: TXT

(Submitter supplied) Rationale: Serial measurements of genome-wide transcriptional changes in alveolar macrophages (AM) and peripheral blood monocytes (PBM) from patients with acute respiratory distress (ARDS) could clarify the biologic programs activated in ARDS and the relationship of these changes to clinical outcomes. Objectives: To identify transcriptional programs activated in purified AM and PBM over the course of ARDS, and determine the relationship of these programs with patient outcomes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

52 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6883 GPL6104

38 Samples

Download data