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Items: 8

1.

Improved DNA methylation analysis via enrichment of demethylated cells expressing an X-inactivated GFP transgene

(Submitter supplied) The demethylating drug 5-aza-2’-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we utilize a novel method that uses the reactivation of an X-inactivated GFP transgene as a marker to enrich for cells that have undergone drug-induced demethylation. By combining it with microarray gene expression profiling, we demonstrate the method’s utility in identifying genes activated by global DNA demethylation. more...
Organism:
Mus musculus
Type:
Expression profiling by array; Methylation profiling by array
Platform:
GPL1261
6 Samples
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Series
Accession:
GSE4718
ID:
200004718
2.

[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 07, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site All probe sets represented on the GeneChip Mouse Expression Set 430 are included on the GeneChip Mouse Genome 430 2.0 Array. more...
Organism:
Mus musculus
601 DataSets
4423 Series
40 Related Platforms
57646 Samples
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Platform
Accession:
GPL1261
ID:
100001261
3.

fibroblast_G-positive_5aza_rep2

Organism:
Mus musculus
Source name:
primary dermal fibroblasts 5aza-treated GFP-positive sorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106540
ID:
300106540
4.

fibroblast_G-positive_5aza_rep1

Organism:
Mus musculus
Source name:
primary dermal fibroblasts 5aza-treated GFP-positive sorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106539
ID:
300106539
5.

fibroblast_G-negative_5aza_rep2

Organism:
Mus musculus
Source name:
primary dermal fibroblasts 5aza-treated GFP-negative sorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106516
ID:
300106516
6.

fibroblast_G-negative_5aza_rep1

Organism:
Mus musculus
Source name:
primary dermal fibroblasts 5aza-treated GFP-negative sorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106505
ID:
300106505
7.

fibroblast_unsorted_control_rep2

Organism:
Mus musculus
Source name:
primary dermal fibroblasts untreated unsorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106430
ID:
300106430
8.

fibroblast_unsorted_control_rep1

Organism:
Mus musculus
Source name:
primary dermal fibroblasts untreated unsorted
Platform:
GPL1261
Series:
GSE4718
Download data
Sample
Accession:
GSM106429
ID:
300106429
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