GSM4339277[Accession] - GEO DataSets

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Items: 3

Select item 2001459161.

RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

(Submitter supplied) In this report, we describe a successful protocol for isolating and expression-profiling live fluorescent- protein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. more...

Organism:: Danio rerio

Type:: Expression profiling by array

Platform:: GPL1319
15 Samples

Download data: CEL

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Accession:: GSE145916

ID:: 200145916

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000013192.

[Zebrafish] Affymetrix Zebrafish Genome Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/projects/geo/info/geo_affy.html The Affymetrix GeneChip Zebrafish Genome Array can be used to study gene expression of over 14,900 Danio rerio transcripts. Sequence information for this array was selected from the following public data sources: RefSeq (July 2003), GenBank (release 136.0, June 2003), dbEST (July 2003), and UniGene (Build 54, June 2003). more...