(Submitter supplied) Purpose:This work aimed to identify the genetic profiles
of piriform projection neurons and characterize their spatial organization within the piriform cortex. Methods: We microdissected the three layers
of pirifrom cortex by laser capture (LMD) and performed RNA deep sequencing in order to identify layer-specific molecular markers, we then validated these data by using RNA in situ hybridization and immunohistochemistry.We next performed anterograde neural tracing experiments to identify piriform target regions, and retrograde neural tracing experiments to analyze how piriform projection neurons are organized within piriform cortex.We then combined the analysis
of patterns
of gene expression with retrograde tracing experiments to identify molecular signatures
of the different subclasses
of piriform projecting neurons.
more...- Organism:
- Mus musculus
- Type:
- Expression profiling by high throughput sequencing
- Platform:
- GPL18480
- 9 Samples
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