GEO DataSets

(Submitter supplied) To estiamte the role lipid flippase subunit Cdc50 plays in Candida glabrata, a Δcdc50 null mutant was constructed based on standard strain ATCC2001 (wild type, WT) through homologous recombination. Then, gene expression profiling analysis was performed using data obtained from RNA-seq of WT and Δcdc50 mutant.

Organism:

Nakaseomyces glabratus CBS 138

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32820

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL30531 GPL22622

28 Samples

Download data: BIGWIG, WIG

(Submitter supplied) To determine the genomic binding sites for the Candida glabrata transcription factor Upc2A, we utilized three different strains. One was the wild-type KKY2001 which contains a wild-type copy of UPC2A but lacks any HA tag. The other two are both derived from KKY2001 but contain a 3X HA tag immediately after the start codon of UPC2A. These strains are BVGC82 and BVGC84, respectively. Both contain a single copy of a loxP element located 252 bp downstream from the UPC2A stop codon. more...

Organism:

Nakaseomyces glabratus CBS 138

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL30531

10 Samples

Download data: WIG

(Submitter supplied) Transcriptional time series of Candida glabrata under iron starvation (SD medium without Fe). Wild type and deletion mutants of the iron-related transcription factors Aft1 and Sef1, as well as of the iron uptake transporter Ftr1 as a positive control.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

60 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in Candida glabrata CBS138 delta-vph2 mutant, compared to the wild-type strain in SC broth (pH5.0 and pH7.4). VPH2 gene encodes a protein that is the assembly factor of a functional V-ATPase. Loss of Vph2p leads to loss of a functional V-ATPase enzyme complex.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL19901

8 Samples

Download data: PAIR

(Submitter supplied) To examine the response of Candida glabrata cells to iron-depleted and iron-repleted environmental conditions, transcriptional profiling analysis was carried out on wild-type and Cghog1∆ cells grown either in presence of BPS or ferric chloride. Genes involved in iron transport and homeostasis, oxidative phosphorylation, amino acid metabolic process and chromatin silencing were found to be differentially regulated.

Organism:

Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL19118

12 Samples

Download data: TXT

(Submitter supplied) Pdr1 is the major regulator of azole resistance in the fungal pathogen Candida glabrata. Earlier experiments demonstrated that expression of Pdr1 itself is increased when cells lose their mitochondrial genome (rho0). Here we use chromatin immunoprecipitation coupled with highthroughput sequencing (ChIP-seq) to map the genomic binding sites for Pdr1 in both normal and rho0 cells. These data provide the first look at genes that are likely to represent the direct targets of Pdr1 in this important pathogen.

Organism:

Nakaseomyces glabratus CBS 138

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19002

9 Samples

Download data: WIG

(Submitter supplied) BG14(wt) and BG14hog1∆ cells were exposed to weak acid (20mM sorbic acid for 20min) to define the role of Hog1 for gene expression during weak acid stress.

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL17933

11 Samples

Download data: TXT

(Submitter supplied) The opportunistic yeast pathogen Candida glabrata is recognized for its ability to acquire resistance during prolonged treatment with azole antifungals. Resistance to azoles is largely mediated by the transcription factor PDR1, resulting in the upregulation of ATP-binding cassette (ABC) transporter proteins and drug efflux. Studies in the related yeast Saccharomyces cerevisiae have shown Pdr1p forms a heterodimer with another transcription factor, Stb5p. more...

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platforms:

GPL8174 GPL10325

13 Samples

Download data: GPR, TXT

(Submitter supplied) This study investigated the effect of milbemycons as efflux inhibitors and antifungal agents. Milbemycin oxims can inhibit growth of Candida glabrata and C. albicans. The effect of milbemycins on transcriptomes was inbvestigated.

Organism:

Candida albicans; Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platforms:

GPL10713 GPL15960

14 Samples

Download data: TXT

(Submitter supplied) To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-a∆ and Cgrtt109∆) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. more...

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL15745

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae; Candida albicans; Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platforms:

GPL11444 GPL10325 GPL7542

20 Samples

Download data: GPR, TXT

(Submitter supplied) [URE3] is a prion (infectious protein) of the Saccharomyces cerevisiae Ure2p, a regulator of nitrogen catabolism. We find that the Ure2p of Candida albicans and C. glabrata also regulate nitrogen catabolism. Conservation of amino acid sequence within the prion domain of Ure2p has been proposed as evidence that the [URE3] prion helps its host. We show that the C. albicans Ure2p, which does not conserve this sequence, can nonetheless form a [URE3] prion, but the C. more...

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10325

8 Samples

Download data: TXT

(Submitter supplied) To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH 2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains. more...

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10927

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10713

33 Samples

Download data: TXT

(Submitter supplied) In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

6 Samples

Download data: TXT

(Submitter supplied) In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles.

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10713

27 Samples

Download data: TXT

(Submitter supplied) Two C. glabrata related isolates recovered from the same patient undergoing azole therapy were characterized. The first isolate, BPY40, is azole-susceptibleand the second, BPY41, is azole-resistant. To determine whether the petite mutation conferred a selective advantage during host infection, the virulence of BPY40 and BPY41 was assessed in mice. Surprisingly, the petite mutant, even if showing in vitro growth deficiency as compared to BPY40, was more virulent than BPY40 both in intravenous and vaginal murine infection models. more...

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

6 Samples

Download data: TXT

(Submitter supplied) Microarray was used to analyze azole resistance of Candida glabrata oropharyngeal isolates from 7 hematopoietic stem cell transplant recipients receiving fluconazole prophylaxis. Transcriptional profiling of the sequential-paired clinical isolates by microarray revealed 19 genes upregulated in the majority of resistant isolates compared to their paired-susceptible isolates. All seven resistant isolates had greater than two fold upregulation of CgPDR1, a master transcriptional regulator of PDR network, and all 7 resistant isolates showed upregulation of known CgPDR1-target genes. more...

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10325

8 Samples

Download data: TXT