GEO DataSets

(Submitter supplied) Host-microbe interactions are virtually bidirectional, benefiting both the host and microbial sides. It is becoming increasingly recognized the influence of the microbe on many aspects of host physiology and diseases, but whether/how the host affects their symbionts is poorly characterized. Here, we reported that the host acts as a critical factor to shape the lifestyle of their symbionts in the Drosophila and bacteria model system. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33401

10 Samples

Download data: TSV

(Submitter supplied) Host-microbe interactions are virtually bidirectional, benefiting both the host and microbial sides. It is becoming increasingly recognized the influence of the microbe on many aspects of host physiology and diseases, but whether/how the host affects their symbionts is poorly characterized. Here, we reported that the host acts as a critical factor to shape the lifestyle of their symbionts in the Drosophila and bacteria model system. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33401

9 Samples

Download data: TXT

(Submitter supplied) The EepR protein is a two-component response regulator protein in the bacterium Serratia marcescens. Mutation of the eepR gene results in pleiotropic changes including reduced expression of secondary metabolites and proteases.

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28109

8 Samples

Download data: TXT

(Submitter supplied) In order to identify mRNA and sRNAs associated with the RNA-binding protein Hfq in Serratia marcescens strain Db10, Hfq-bound RNA was immunoprecipitated from a strain encoding an Hfq-3FLAG fusion protein at the normal location and sequenced, in parallel with the wild type strain (no fusion) as negative control. Additionally global transcriptional start site mapping was performed on total RNA, with or without TEX treatment, isolated from wild type Serratia marcescens. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21500

4 Samples

Download data: WIG

(Submitter supplied) The goal of this project was to analyze virulence factor expression during microbial keratitis and to determine the impact of the Rcs system regulator GumB on the transcriptional profile

Organism:

Oryctolagus cuniculus; Serratia marcescens

Type:

Other

Platform:

GPL28651

12 Samples

Download data: RCC

(Submitter supplied) The goal of this project was to analyze the role of three different transcriptional regulator systems in regulation of gene expression

Organism:

Serratia marcescens

Type:

Other

Platform:

GPL28651

24 Samples

Download data: RCC, TXT

(Submitter supplied) The GumB protein is an IgaA-family member that negatively regulates the Rcs stress response system in the bacterium Serratia marcescens. Mutation of the gumB gene results in increased RCs system activity of numerous genes including those involved in flagellar based motility and capsular polysaccharide formation.

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28573

6 Samples

Download data: TXT

(Submitter supplied) We report the application of transcriptome sequencing technology for high-throughput profiling of Serratia marcescens for producing prodigiosin. By obtaining over 163 million bases of sequence from Serratia marcescens genome DNA, we generated transcriptome -state maps of Serratia marcescens 12h cells, 24h cells, and 36h cells at 30C and 37C,respectively. We explored the mechanism of S. marcescens response temperature regulation at the transcription level through transcriptome sequencing technology. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28109

12 Samples

Download data: TXT

(Submitter supplied) RNAseq analysis was performed to evaluate gene expression differences between strains JNB5-1 and SK8-37. S. marcescens JNB5-1 and SK8-37 cells were grown in LB medium for 10 h before harvesting. The collected cells were then treated with RNAprep pure Kit (TIANGEN) to extract total bacterial RNA and delivered to GENEWIZ in dry ice for transcriptome resequencing analysis. For annotation, the genome of S. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26806

2 Samples

Download data: XLSX

(Submitter supplied) Serratia marcescens is a bacterium frequently found in the environment, but over the last several decades it has evolved into a concerning clinical pathogen, causing fatal bacteremia. To establish such infections, pathogens require specific nutrients; one very limited but essential nutrient is iron. We sought to characterize the iron acquisition systems in S. marcescens isolate UMH9, which was recovered from a clinical bloodstream infection. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28109

4 Samples

Download data: XLSX

(Submitter supplied) RNAseq analysis was performed to evaluate gene expression differences between strains JNB5-1 and SK68. S. marcescens JNB5-1 and SK68 cells were grown in LB medium for 4 h before harvesting. The collected cells were then treated with RNAprep pure Kit (TIANGEN) to extract total bacterial RNA and delivered to GENEWIZ in dry ice for transcriptome resequencing analysis. For annotation, the genome of S. marcescens WW4 (NC_020211.1) was used as reference. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26806

2 Samples

Download data: TXT

(Submitter supplied) RNAseq analysis was performed to evaluate gene expression differences between strains JNB5-1 and ZK66. S. marcescens JNB5-1 and ZK66 cells were grown in LB medium for 12 h before harvesting. The collected cells were then treated with RNAprep pure Kit (TIANGEN) to extract total bacterial RNA and delivered to GENEWIZ in dry ice for transcriptome resequencing analysis. For annotation, the genome of S. marcescens WW4 (NC_020211.1) was used as reference. A total of 24048918 reads matched to the referenced genome in the sample of JNB5-1, and 24569696 reads in the sample of ZK66. The differentially expressed genes (DEGs) were determined between strains JNB5-1 and ZK66 with the standards of false discovery rate (FDR) ≤ 0.05, fold change |log2Ratio|≥1. Transcriptome data showed that expression of 641 genes were upregulated while 784 genes were down regulated by at least 2-fold when comparing the metR mutant strain ZK66 to its parent strain JNB5-1. Based on the annotation of KEGG_B_class, the up-regulated and down-regulated genes were classified into 30 and 27 major cellular processes, respectively. The transcriptome data indicated that MetR may regulate a variety of cellular processes in S. marcescens, including prodigiosin synthesis, and cell motility.

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26806

2 Samples

Download data: TXT

(Submitter supplied) Global amphibian declines and extinction events are currently occurring at an unprecedented rate. While various factors are influencing these declines, one factor that is readily identifiable is disease. Specifically, the fungal pathogen Batrachochytrium dendrobatidis is thought to play a major role in amphibian declines in tropical and neotropical regions of the globe. While the effects of this chytrid fungus have been shown to be devastating, certain individuals and relict populations have shown resistance. more...

Organism:

Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22119

6 Samples

Download data: TXT

(Submitter supplied) DB11 genes are from GenBank: HG326223.1 (https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/nuccore/HG326223.1). A few of the genes were not in the Db11 genome and these were designated by orf#. Protocol: http://www.nanostring.com

Organism:

Serratia marcescens

2 Series

36 Samples

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