GEO DataSets

(Submitter supplied) The human transcription factor (TF) CGGBP1 (“CGG Binding Protein”) is conserved only in amniotes, and is believed to derive from the zf-BED and Hermes transposase DNA-binding domains (DBDs) of a hAT DNA transposon. Here, we examine the DNA binding preferences of a wide variety of metazoan CGGBP1-like TFs with this bipartite domain using PBM experiments. The derived motifs are available at ...

Organism:

Aedes aegypti; Culex quinquefasciatus; Latimeria chalumnae; Cyprinus carpio; synthetic construct; Hucho hucho; Amphiprion ocellaris; Labeo rohita; Mola mola; Sphaeramia orbicularis; Anabarilius grahami; Acyrthosiphon pisum; Salmo salar; Fundulus heteroclitus; Sparus aurata; Chrysemys picta; Austrofundulus limnaeus; Anabas testudineus; Amphiprion percula; Salarias fasciatus; Petromyzon marinus; Acipenser ruthenus; Gallus gallus; Erpetoichthys calabaricus; Rhizophagus irregularis; Paramormyrops kingsleyae; Locusta migratoria; Crocodylus porosus; Homo sapiens; Rhodnius prolixus; Rhipicephalus appendiculatus; Kryptolebias marmoratus; Nematostella vectensis; Acanthochromis polyacanthus; Hippocampus comes; Hofstenia miamia

Type:

Other

Platform:

GPL11260

258 Samples

Download data: TXT

(Submitter supplied) DNA methylation is an important chromatin modification that is necessary for the structural integrity and proper regulation of the genome for many species. Despite its conservation across the tree of life, little is known about its contribution to complex traits. Reports that differences in DNA methylation between castes in closely related Hymenopteran insects (ants, bees and wasps) contributes to social behaviors has generated hypotheses on the role of DNA methylation in governing social behavior. more...

Organism:

Blattella germanica; Periplaneta americana; Helicoverpa zea; Aedes albopictus; Aedes atropalpus; Rhyparobia maderae; Cryptocercus garciai; Aphis craccivora; Heliothis virescens; Copidosoma floridanum; Eublaberus posticus; Reticulitermes flavipes; Microplitis demolitor; Polistes carolina; Blattella asahinai; Aedes aegypti; Anopheles gambiae; Culex quinquefasciatus; Reticulitermes virginicus; Pemphigus obesinymphae; Blaptica dubia; Pemphigus populitransversus; Microplitis mediator; Chrysodeixis includens; Acyrthosiphon pisum; Anastrepha suspensa; Aphidius ervi; Toxorhynchites amboinensis; Pemphigus populicaulis; Pyractomena borealis

Type:

Methylation profiling by high throughput sequencing

30 related Platforms

69 Samples

Download data: TXT

(Submitter supplied) Purpose: Identify differentially expressed genes between 5 pea aphid morphs Methods: Collected whole bodies of 30 adult aphids of each of the five pea morphs and three clones (total of 15 samples)

Organism:

Acyrthosiphon pisum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18577

15 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Acyrthosiphon pisum

Type:

Non-coding RNA profiling by high throughput sequencing; Non-coding RNA profiling by array

Platforms:

GPL9994 GPL9993

10 Samples

Download data: FA, XLS

(Submitter supplied) Bioinformatic prediction, deep sequencing of microRNA and expression analysis during phenotypic plasticity in the pea aphid acyrthosiphon pisum We developed high throughput Solexa sequencing and bioinformatic analyses of the genome of the pea aphid Acyrthosiphon pisum in order to identify the first miRNAs from a hemipteran insect. By combining these methods we identified 155 miRNAs including 56 conserved and 99 new miRNAs. more...

Organism:

Acyrthosiphon pisum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9994

1 Sample

Download data: FA

(Submitter supplied) We developed high throughput Solexa sequencing and bioinformatic analyses of the genome of the pea aphid Acyrthosiphon pisum in order to identify the first miRNAs from a hemipteran insect. By combining these methods we identified 155 miRNAs including 56 conserved and 99 new miRNAs. Moreover, we investigated the regulation of these miRNAs in different alternative morphs of the pea aphid by analysing the expression of miRNAs across the switch of reproduction mode.

Organism:

Acyrthosiphon pisum

Type:

Non-coding RNA profiling by array

Platform:

GPL9993

9 Samples

Download data: XLS

(Submitter supplied) Aphid adaptation to harsh winter conditions is illustrated by an alternation of their reproductive mode. Aphids detect photoperiod shortening by sensing the length of the night and switch from viviparous parthenogenesis in spring and summer, to oviparous sexual reproduction in autumn. The photoperiodic signal is transduced from the head to the reproductive tract to change the fate of the future oocytes from mitotic diploid embryogenesis to haploid formation of gametes. more...

Organism:

Acyrthosiphon pisum

Type:

Expression profiling by array

Platform:

GPL8426

24 Samples

Download data: GPR

(Submitter supplied) The pea aphid, Acyrthosiphon pisum, exhibits several environmentally cued polyphenisms, in which discrete, alternative phenotypes are produced. At low density parthenogenetic females produce unwinged female progeny, but at high density females produce progeny that develop with wings. These alternative phenotypes represent a solution to the competing demands of dispersal and reproduction. Males also develop as either winged or unwinged, but these alternatives are determined by a genetic polymorphism. more...

Organism:

Buchnera aphidicola; Escherichia coli; Acyrthosiphon pisum

Type:

Expression profiling by array

Platform:

GPL5347

24 Samples

Download data: GPR, TXT

(Submitter supplied) Seasonal photoperiodism is responsable in aphid for the switch from asexual to sexual reproduction. In order to identify genes regulating the photoperiodic response, a cDNA microarray from the pea aphid was used to compare RNA populations from short- and long-day reared aphids. Analyses revealed that 64 different transcripts were significantly regulated, with a strong biological signature for cuticular proteins and proteins involved in cellular signalling or signal transduction. more...

Organism:

Acyrthosiphon pisum

Type:

Expression profiling by array

Platform:

GPL4580

12 Samples

Download data

(Submitter supplied) Background: The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont Buchnera aphidicola. Buchnera inhabits specialized host cells called bacteriocytes, provides nutrients to the aphid and has co-speciated with its aphid hosts for the past 150 million years. We have used a single microarray to examine gene expression in the pea aphid, Acyrthosiphon pisum, and its resident Buchnera. more...

Organism:

Buchnera aphidicola; Acyrthosiphon pisum

Type:

Expression profiling by array

Platform:

GPL2889

1 Sample

Download data: CSV

(Submitter supplied) http://www.lcsciences.com/support/technology/technology.html

Organism:

Acyrthosiphon pisum

2 Series

9 Samples

Download data

(Submitter supplied) Corning UltraGAPS II slides (Corning, NY, USA). Spotted by a Microgrid II spotter (Biorobotics, Cambridge, UK) Protocol: cDNAs were amplified from bacterial lysates and PCR products were resuspended into 20 µl of 1.5 M betaine, 3x SSC buffer

Organism:

Acyrthosiphon pisum

1 Series

24 Samples

Download data

(Submitter supplied) Dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont, Buchnera aphidicola. Protocol: Genes were arranged in duplicate spots on slides, with Buchnera genes grouped into the first and second of 24 subarrays. In addition to spot duplication, the entire set was printed twice on each slide with the result that each gene was represented by four spots in total.

Organism:

Buchnera aphidicola; Escherichia coli; Acyrthosiphon pisum

1 Series

24 Samples

Download data

(Submitter supplied) Corning UltraGAPS II slides (Corning, NY, USA). Spotted by a Microgrid II spotter (Biorobotics, Cambridge, UK) Protocol: cDNAs were amplified from bacterial lysates and PCR products were resuspended into 20 µl of 1.5 M betaine, 3x SSC buffer.

Organism:

Acyrthosiphon pisum

1 Series

12 Samples

Download data

(Submitter supplied) Dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont, Buchnera aphidicola The platform table is condensed to show each GENE on the array. The full array table, showing all spots, is linked as a supplementary file (see download link below). Protocol: Genes were arranged in duplicate spots on slides, with Buchnera genes grouped into the first and second of 24 subarrays. more...

Organism:

Buchnera aphidicola; Acyrthosiphon pisum

1 Series

1 Sample

Download data: TXT