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Items: 1 to 20 of 735

1.

Transcriptome of Tribolium castaneum selection lines (control-, drought-, heat-, heat/drought-adapted) under different climatic conditions

(Submitter supplied) We studied plastic and evolutionary responses in gene expression of Tribolium castaneum after beetles’ exposure to new environments that differed from ancestral control conditions in temperature, humidity or both. Using experimental evolution with ten replicated lines per condition, we were able to demonstrate adaptation (higher offspring number compared to control lines) after 20 generations. We measured whole-transcriptome gene expression with RNA-seq to infer evolutionary and plastic changes. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20193
244 Samples
Download data: TXT
Series
Accession:
GSE156256
ID:
200156256
2.

Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum

(Submitter supplied) Background: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18162
6 Samples
Download data: TXT
Series
Accession:
GSE155634
ID:
200155634
3.

Tribolium castaneum experimental evolution

(Submitter supplied) We report Illumina-generated RNASeq data of several populations of Tribolium castaneum larvae selected for higher or lower immune priming specificity as well as unselected control populations. From each of these populations, we injected groups of 20 larvae with either one of three bacteria species or left them untreated as controls. Whole body samples were taken 6h after injection and used for RNASeq Analysis.
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18162
60 Samples
Download data: TXT
Series
Accession:
GSE133892
ID:
200133892
4.

Transcriptome of Tribolium castaneum in different climatic conditions

(Submitter supplied) We combined fitness and whole-transcriptome data of Tribolium castaneum to investigate gene expression and fitness responses to drought, heat and their combination. Fitness was reduced by both stressors and their combined effects were nearly additive. Accordingly, expression data showed that both stressors were acting mostly independently and did not interfere physiologically. Response to heat stress resulted in a much higher number of differentially expressed genes compared to drought stress. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20193
183 Samples
Download data: TXT
Series
Accession:
GSE133570
ID:
200133570
5.

Differentially and co-expressed genes in embryo, germ-line and somatic tissues of Tribolium castaneum

(Submitter supplied) Transcriptomic studies of Tribolium castaneum have led to significant advances in our understanding of co-regulation and differential expression of genes in development. However, previously used microarray approaches have covered only a subset of known genes. The aim of this study was to investigate gene expression patterns of beetle embryo, germ-line and somatic tissues. We identified 12,302 expressed genes and determined differentially expressed up and down-regulated genes among all samples. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20193
17 Samples
Download data: CSV, TAR
Series
Accession:
GSE119739
ID:
200119739
6.

Dnmt1 has an essential function despite the absence of CpG DNA methylation in the red flour beetle Tribolium castaneum

(Submitter supplied) Epigenetic mechanisms, such as CpG DNA methylation enable phenotypic plasticity and rapid adaptation to changing environments. CpG DNA methylation is established by DNA methyltransferases (DNMTs), which are well conserved across vertebrates and invertebrates. There are insects with functional DNA methylation despite lacking a complete set of Dnmts. But at least one of the enzymes, DNMT1, appears to be required to maintain an active DNA methylation system. more...
Organism:
Tribolium castaneum
Type:
Methylation profiling by high throughput sequencing
Platform:
GPL18255
2 Samples
Download data: TXT
Series
Accession:
GSE120735
ID:
200120735
7.

Next Generation Sequencing to determine the fundamental mechanisms of Tribolium castaneum larvae to cope with hypoxia

(Submitter supplied) We used high-throughput sequencing technology to determine the fundamental mechanisms of Tribolium castaneum to cope with hypoxia. Totals of 12,524,554 useful reads from the control group and 14,193,339 useful reads from the treatment group were generated after Q20 filtering. A total of 16,524 unigenes had been annotated. More than 300 transcripts showed differential expression between the control and hypoxia group, with a fold change ≥1.5 and p value <0.05. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20193
2 Samples
Download data: GTF
Series
Accession:
GSE114049
ID:
200114049
8.

Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum

(Submitter supplied) Evolution of cis-properties (such as enhancers) often plays an important role in the production of diverse morphology. However, a mechanistic understanding is often limited by the absence of methods to study enhancers in species outside of established model systems. Here, we sought to establish methods to identify and test enhancer activity in the red flour beetle, Tribolium castaneum. To identify possible enhancer regions, we first obtained genome-wide chromatin profiles from various tissues and stages of Tribolium via FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)-sequencing. more...
Organism:
Tribolium castaneum
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL18255
16 Samples
Download data: BW, NARROWPEAK
Series
Accession:
GSE104495
ID:
200104495
9.

miRNA expression in Anopheles gambiae tissues

(Submitter supplied) Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. more...
Organism:
Bombyx mori; Drosophila melanogaster; Tribolium castaneum; Aedes aegypti; Anopheles gambiae; Culex quinquefasciatus; Apis mellifera
Type:
Non-coding RNA profiling by array
Platform:
GPL23950
40 Samples
Download data: TXT
Series
Accession:
GSE103034
ID:
200103034
10.

The single-base resolution DNA methylation profiles for four developmental stages of Tribolium castaneum

(Submitter supplied) Cytosine DNA methylation in the Tribolium castaneum has been controversial, its distribution pattern and functions have not been established yet. Here, using BS-Seq, we confirmed the existence of DNA methylation and described the methylation profiles for four life stages of T.castaneum. Interestingly, two types of DNA methylation had been discovered in T.castaneum genome. One was the symmetrical CpG methylation which was typically existed among eukaryotes methylomes, but occupied small part in methylome of T. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing
Platform:
GPL22138
8 Samples
Download data: TAR, TXT
Series
Accession:
GSE84253
ID:
200084253
11.

The within-host dynamics of infection in trans-generationally primed beetles

(Submitter supplied) We report the temporal dynamics of differential gene expression between primed and unprimed beetles infected with the entomopathogen Bt
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18255
48 Samples
Download data: TXT
Series
Accession:
GSE95387
ID:
200095387
12.

High-throughput sequencing identify miRNAs of Tribolium castaneum

(Submitter supplied) To identify miRNAs of Tribolium castaneum, one small RNA libraries for mix samples of eight development stage (including 1-day-old early embryo, 3-day-old late embryo, 1-day-old early larva, 20-day-old late larva, 1-day-old early pupa, 6-day-old late pupa, 1-day-old early adult, 7-day-old late adult) were constructed. Totally, 12,259,974 raw reads were obtained, 7,116,806 mappable reads were remained and 2,175,311 high-quality miRNA reads were identified after the small RNA digitalisation analysis. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL9920
1 Sample
Download data: XLSX
Series
Accession:
GSE81172
ID:
200081172
13.

Effect of maternal treatment on gene expression differences in Bacillus thuringiensis infected pooled larvae

(Submitter supplied) This study examines gene expression differences between infected larvae from different maternal infection backgrounds to try to understand the immunological basis of invertebrate immune priming. Female Tribolium castaneum beetles were either jabbed with sterile saline, heat killed Bt, or left naïve. 15 Offspring from the first and second broods were infected with Bt and 24 hours later pooled by treatment and flash frozen Beetles from saline and primed maternal backgrounds are compared to naïve infected
Organism:
Tribolium castaneum
Type:
Expression profiling by array
Platform:
GPL16756
2 Samples
Download data: TXT
Series
Accession:
GSE44912
ID:
200044912
14.

Survey of methylomes of two beetles, Nicrophorus vespilloides and Tribolium castaneum

(Submitter supplied) The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole genome bisulfite sequencing to assay if DNA methylation was present in another beetle, Nicrophorus vespilloides. We used T. castaneum as a negative control.
Organism:
Nicrophorus vespilloides; Tribolium castaneum
Type:
Methylation profiling by high throughput sequencing
Platforms:
GPL20192 GPL20193
6 Samples
Download data: TSV, TXT
Series
Accession:
GSE68814
ID:
200068814
15.

Embryonic small RNA and RNA profiles of Tribolium castaneum and Drosophila oocytes

(Submitter supplied) Next generation sequencing analysis of the small RNA and RNA profiles of successive time intervals during T. castaneum embryogenesis, and Drosophila unfertilized oocytes
Organism:
Tribolium castaneum; Drosophila virilis; Drosophila melanogaster
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
4 related Platforms
22 Samples
Download data: TXT
Series
Accession:
GSE63770
ID:
200063770
16.

Tissue-specific transcriptomics, chromosomal localization, and phylogeny of chemosensory and odorant binding proteins from the red flour beetle Tribolium castaneum reveal subgroup specificities for olfaction or more general functions

(Submitter supplied) Chemoreception is based on the senses of smell and taste that are crucial for animals to find new food sources, shelter, and mates. The initial step in olfaction involves the translocation of odorants from the periphery through the aqueous lymph of the olfactory sensilla to the odorant receptors by chemosensory proteins (CSPs) or odorant binding proteins (OBPs).To better understand the roles of CSPs and OBPs in a coleopteran pest species, the red flour beetle Tribolium castaneum (Coleoptera, Tenebrionidae), we performed transcriptome analyses of male and female antennae, heads, mouthparts, legs, and bodies, which revealed that all 20 CSPs and 49 of the 50 previously annotated OBPs are transcribed. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18255
16 Samples
Download data: FA, TXT
Series
Accession:
GSE63162
ID:
200063162
17.

The extraembryonic serosa is a frontier epithelium providing the insect egg with a full-range innate immune response

(Submitter supplied) Drosophila larvae and adults possess a potent innate immune response, but the response of their eggs is particularly poor. Here we show that eggs of the beetle Tribolium castaneum, in contrast, possess a full range of immune defence mechanisms, based on complete transcriptome comparisons of naïve, sterilely injured, and bacterially challenged eggs. Upon infection, we find massive upregulation of AMPs and differential regulation of 375 other genes including both IMD and Toll signalling components. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18162
27 Samples
Download data: TXT
Series
Accession:
GSE54018
ID:
200054018
18.

Wnt/β-catenin signaling integrates patterning and metabolism of the insect growth zone.

(Submitter supplied) Purpose: Combination of systemic RNAi with RNAseq to identify target genes of important pathways in early development Wnt/β-catenin and hedgehog (Hh) signaling are essential for transmitting signals across cell membranes in animal embryos. Early patterning of the principal insect model, Drosophila melanogaster, occurs in the syncytial blastoderm, where diffusion of transcription factors obviates the need for signaling pathways. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18255
25 Samples
Download data: TXT
Series
Accession:
GSE54706
ID:
200054706
19.

Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum

(Submitter supplied) Using a tiled whole-genome microarray, we found that 58.2% of Tribolium castaneum genes are maternally loaded into eggs. Comparison of known Drosophila melanogaster maternal genes to our results showed widespread conservation of maternal function with T. castaneum. We also found many T. castaneum genes with previously identified gender or tissue specific expression were also maternally loaded into eggs. more...
Organism:
Tribolium castaneum
Type:
Expression profiling by genome tiling array
Platforms:
GPL15612 GPL15613 GPL15614
9 Samples
Download data: PAIR, TXT
Series
Accession:
GSE38245
ID:
200038245
20.

Deep sequencing of small RNAs from Tribolium castaneum

(Submitter supplied) Characterization of miRNAs in red flour beetle Tribolium castaneum by deep sequencing of two different RNA libraries.
Organism:
Tribolium castaneum
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL11311
2 Samples
Download data
Series
Accession:
GSE26036
ID:
200026036
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