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Items: 1 to 20 of 667

1.

Comparative analysis of testis transcriptome between a genetic male sterile line (GMS) and its wild-type 898WB in silkworm, Bombyx mori

(Submitter supplied) Purpose:The purpose of study these processes, is helpful to understand the mechanisms of genetic sterility of lepidopteran insects and achieve sterile insect technique (SIT) for pest control. Methods:Intact testes were dissected on day 5 of fifth-instar larvae and immediately frozen in liquid nitrogen for RNA-Seq. The testes from three silkworms were taken as one biological sample, and three samples per strain were prepared as biological replicates. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30062
6 Samples
Download data: TXT
Series
Accession:
GSE191033
ID:
200191033
2.

Differentially expressed genes (DEGs) in oviducts of Yun7 and its giant egg mutant Yun7Ge of silkworm, Bombyx mori

(Submitter supplied) Yun7Ge (Yun7Ge is represented by Ge in the high-throughput sequencing results) is a giant egg mutant found in the silkworm variety Yun7 (Yun7 is represented by Se in the high-throughput sequencing results). The amount of laid eggs and the hatching rate of Yun7Ge are reduced, which is not conducive to the reproduction. The target gene controlling giant egg trait is located on the Z chromosome (chromosome 1) was determined through genetic analysis. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30062
4 Samples
Download data: TXT
Series
Accession:
GSE173672
ID:
200173672
3.

RNA m6A methylation of 3ʹ splice site prevents binding of U2AF35 to inhibit splicing

(Submitter supplied) OThe N6-methyladenosine (m6A) RNA modification is widely used to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (orthologue of mouse METTL16) deposits an m6A mark on the 3′ splice site (AG) of the SAM synthetase pre-mRNA which inhibits its proper splicing and protein production. The mechanism is triggered by a rich diet, and acts as an m6A-mediated switch to stop SAM production and regulate its homeostasis. more...
Organism:
Caenorhabditis elegans; Mus musculus; Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL22765 GPL19757 GPL28266
42 Samples
Download data: BW, CSV, XLSX
Series
Accession:
GSE146873
ID:
200146873
4.

Precocious metamorphosis of silkworm by BmNPV infection in the latter half of the fifth instar larvae

(Submitter supplied) The silkworm, Bombyx mori, is a complete metamorphosis insect and an economically important for silk production, the model to study insect physiology and biochemistry. Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of the silkworm and its host range is restricted to silkworm larvae, requiring interaction with silkworm larvae to accomplish virus replication. Prothoracic glands (PGs) are a model for synthetic ecdysone with regulating insect growth and development. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL23127
4 Samples
Download data: TXT, XLSX
Series
Accession:
GSE167875
ID:
200167875
5.

High throughput sequencing of Siwi associated piRNAs from Bombyx mori BmN4 cells

(Submitter supplied) RNAs associating with PIWI proteins were Immunoisolated from BmN4 cells. Sequence libraries were generated with NEBNext Small RNA Library Prep Set for Illumina(NEB). Libraries were sequenced using Illumina MiSeq (single-end, 51 cycles).
Organism:
Bombyx mori
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL18754
2 Samples
Download data: TXT
Series
Accession:
GSE161405
ID:
200161405
6.

Formation of the CenH3-deficient holocentromere in Lepidoptera avoids actie chromatin

(Submitter supplied) Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring respectively to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). more...
Organism:
Trichoplusia ni; Bombyx mori
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
5 related Platforms
41 Samples
Download data: BW
Series
Accession:
GSE158902
ID:
200158902
7.

RNA associating with PIWI proteins in Bombyx mori BmN4 cells

(Submitter supplied) RNAs associating with PIWI proteins were Immunoisolated from BmN4 cells. Sequence libraries were generated with NEBNext Small RNA Library Prep Set for Illumina(NEB). Libraries were sequenced using Illumina MiSeq (single-end, 51 cycles).
Organism:
Bombyx mori
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL18754
9 Samples
Download data: FA, TXT, XLSX
Series
Accession:
GSE150444
ID:
200150444
8.

Hierarchical roles of mitochondrial PAPI and Zucchini in Bombyx germline piRNA biogenesis

(Submitter supplied) PIWI-interacting RNAs (piRNAs) are germline-enriched small RNAs that control transposons to maintain genome integrity1,2,3. To achieve this, piRNAs bind PIWI proteins upon being processed from piRNA precursors1,2,3. Bioinformatic studies of piRNA biogenesis in Drosophila showed that the piRNA 5′ end is formed by PIWI-Slicer or Zucchini (Zuc) endonucleolytic cleavage, while the 3′ end is formed by Zuc or Nibbler (Nbr) 3′-to-5′ exonucleolytic activity4,5,6. more...
Organism:
Bombyx mori
Type:
Other; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL18754
8 Samples
Download data: TXT
Series
Accession:
GSE107371
ID:
200107371
9.

Identify potential maternal genes of Bombyx mori using Digital Gene Expression Profiling

(Submitter supplied) Maternal genes present in mature oocytes and play a crucial role in the early development of the silkworm. Although maternal genes have been widely studied in many species, there are limited research in Bombyx mori. High-through experimental technology provides a practical way for genome-wide study. Here we show a transcriptome study on silkworm eggs. Unfertilized eggs from five different stages of early development were used for detecting the change situation of gene expression. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16216
5 Samples
Download data: XLS, XLSX
Series
Accession:
GSE109815
ID:
200109815
10.

MicroRNA differentially expressed in diapausing versus HCl-treated Bombyx embryos

(Submitter supplied) Differentially expressed microRNAs (miRNAs) were detected to explore the molecular mechanisms of diapause termination. The total small RNA (sRNA) of diapause-destined silkworm eggs and HCl-treated eggs was extracted and then sequenced using HiSeq/MiSeq high-throughput method. 44 novel miRNAs were discovered. Compared to those in the diapause-destined eggs, 61 miRNAs showed significant changes in the acid-treated eggs, with 23 being up-regulated and 38 being down-regulated. more...
Organism:
Bombyx mori
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL16216
2 Samples
Download data: TXT
Series
Accession:
GSE95576
ID:
200095576
11.

miRNA expression in Anopheles gambiae tissues

(Submitter supplied) Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. more...
Organism:
Tribolium castaneum; Aedes aegypti; Anopheles gambiae; Culex quinquefasciatus; Apis mellifera; Bombyx mori; Drosophila melanogaster
Type:
Non-coding RNA profiling by array
Platform:
GPL23950
40 Samples
Download data: TXT
Series
Accession:
GSE103034
ID:
200103034
12.

Differentially expressed genes in the head of the 2nd instar pre-molting larvae of the nm2 mutant of the silkworm, Bombyx mori

(Submitter supplied) Molting is an important physiological process in the larval stage of Bombyx mori and is controlled by various hormones and peptides. The silkworm mutant that exhibits the phenotype of non-molting in the 2nd instar (nm2) is incapable of molting in the 2nd instar and dies after seven or more days. The ecdysone titer in the nm2 mutant is lower than that in the wildtype, and the mutant can be rescued by feeding with 20E and cholesterol. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16216
2 Samples
Download data: XLSX
Series
Accession:
GSE98972
ID:
200098972
13.

Transcriptome analysis of the epidermis of the purple quail-like (q-lp) mutant of silkworm, Bombyx mori

(Submitter supplied) A new purple quail-like (q-lp) mutant found from the plain silkworm strain 932VR has pigment dots on the epidermis similar to the pigment mutant quail (q). In addition, q-lp mutant larvae are inactive, consume little and grow slowly, with a high death rate and other developmental abnormalities. Pigmentation of the silkworm epidermis consists of melanin, ommochrome and pteridine. Silkworm development is regulated by ecdysone and juvenile hormone. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL23127
2 Samples
Download data: XLS
14.

Host- parasite interaction: Transcriptome responses to parasitism in the silkworm Bombyx mori

(Submitter supplied) To reveal the variations in transcriptome responses of hemocytes of B. mori after the parasitic infection.
Organism:
Bombyx mori
Type:
Expression profiling by array
Platform:
GPL20728
4 Samples
Download data: TXT
Series
Accession:
GSE75520
ID:
200075520
15.

Genome-wide transcriptional analysis of PTTH-stimulated Bombyx prothoracic gland

(Submitter supplied) This is part of a cross-species approach to characterize transcriptional response of the insect prothoracic gland (PG) to the prothoracicotropic hormone (PTTH). Genome-wide response of the PG to PTTH was analyzed in vitro in Bombyx and in vivo in Drosophila, and the results were compared to identify common components in the PTTH signaling pathway.
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16216
4 Samples
Download data: XLSX
Series
Accession:
GSE84963
ID:
200084963
16.

A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL9151
14 Samples
Download data: GTF, XLS
Series
Accession:
GSE71992
ID:
200071992
17.

Identification of genome wide targets of the hox protein Ultrabithorax (Ubx) in larval wing buds of Bombyx mori (Daizo)

(Submitter supplied) Amongst the various different insect groups, there is remarkable diversity in the number and size of wings. However the development of the basic body plan in insects is similar to a large extent. The genes of the hox complex regulate various pathways to bring about the development or modification of different organs. Ubx, a gene of the bithorax hox complex is expressed in the third thoracic segment of insects and is known to specify the fate of wing appendage in that segment.To understand the role of Ubx and how its regulatory mechanism has evolved through the course of evolution we have compared its genome wide targets in different insect orders. more...
Organism:
Bombyx mori
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL9151
12 Samples
Download data: BED, XLS
Series
Accession:
GSE71990
ID:
200071990
18.

Identification of differentially expressed genes in fore and hind wing buds of IV instar larvae of Bombyx mori (Daizo)

(Submitter supplied) Amongst the various different insect groups, there is remarkable diversity in the number and size of wings. However the development of the basic body plan in insects is similar to a large extent. The genes of the hox complex regulate various pathways to bring about the development or modification of different organs. Ubx, a gene of the bithorax hox complex is expressed in the third thoracic segment of insects and is known to specify the fate of wing appendage in that segment.To understand the role of Ubx and how its regulatory mechanism has evolved through the course of evolution we have compared its genome wide targets in different insect orders. more...
Organism:
Bombyx mori
Type:
Expression profiling by high throughput sequencing
Platform:
GPL9151
2 Samples
Download data: DIFF, GTF
Series
Accession:
GSE71988
ID:
200071988
19.

Genome wide microarray based expression profiles associated with BmNPV resistantance and susceptibility in Indian silkworm races of Bombyx mori

(Submitter supplied) The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in the midgut tissues of BmNPV infected resistant (Sarupat) and susceptible (CSR-2) Indian silkworm races. In the resistant race, 735 genes were upregulated and 589 genes were down regulated at 12 hours of BmNPV post infection. Similarly in case of susceptible race, 2183 genes were up regulated and 2115 genes were down regulated. more...
Organism:
Bombyx mori
Type:
Expression profiling by array
Platform:
GPL20728
8 Samples
Download data: TXT
Series
Accession:
GSE71240
ID:
200071240
20.

The insect prothoracic gland as a model for steroid hormone biosynthesis and regulation

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Bombyx mori; Drosophila melanogaster
Type:
Expression profiling by array; Expression profiling by high throughput sequencing
Platforms:
GPL16216 GPL15486
46 Samples
Download data: PAIR, XYS
Series
Accession:
GSE80485
ID:
200080485
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