GEO DataSets

(Submitter supplied) Ciona intestinalis is an invertebrate animal model system that is well characterized and has many advantages for the study of cardiovascular biology. The regulatory mechanisms of cardiac myocyte proliferation in Ciona are intriguing since Ciona are capable of regeneration throughout their lifespan. To identify important regeneration factors in Ciona, microarray analysis was conducted on RNA from adult Ciona hearts with normal or damaged myocardium using custom Affymetrix GeneChips. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by array

Platform:

GPL15657

12 Samples

Download data: CEL, CHP

(Submitter supplied) Thalidomide has a dark history as a teratogen, but in recent years its derivates have been shown to function as a chemotherapeutic agent. These drugs bind cereblon (CRBN), the substrate receptor of an E3 ubiquitin ligase complex and modify its degradation targets. Despite these insights, remarkably little is known about the normal function of cereblon in development. Here we employ Ciona, an invertebrate chordate, to identify endogenous Crbn targets. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29701

2 Samples

Download data: H5

(Submitter supplied) We mapped DNA methylation in 580 animal species (535 vertebrates, 45 invertebrates), resulting in 2443 genome-scale, base-resolution DNA methylation profiles of primary tissue samples from various organs. Reference-genome independent analysis of this comprehensive dataset defined a “genomic code” of DNA methylation, which allowed us to predict global and locus-specific DNA methylation from the DNA sequence within and across species. more...

Organism:

Riftia pachyptila; Homarus americanus; Pisaster brevispinus; Negaprion brevirostris; Danio rerio; Esox lucius; Gadus morhua; Myzopsetta ferruginea; Chelydra serpentina; Emydidae; Graptemys; Varanus exanthematicus; Naja; Vipera ammodytes; Dromaius novaehollandiae; Columba livia; Falco peregrinus; Haliaeetus albicilla; Serinus; Phalacrocorax carbo; Macropodidae; Erinaceidae; Leontocebus fuscicollis; Saguinus mystax; Cercopithecus; Vulpes vulpes; Ursus; Ursus arctos; Procyon lotor; Meles meles; Felis catus; Tayassuidae; Cervidae; Cervus nippon; Muntiacus; Ammotragus; Bos; Boselaphus tragocamelus; Bubalus; Cricetinae; Caviidae; Hydrochoerus hydrochaeris; Heterocephalus; Macroscelidea; Macroscelides proboscideus; Dolichotis; Duttaphrynus melanostictus; Corvus corone; Strigiformes; Vicugna pacos; Yinpterochiroptera; Acinonyx; Colobus guereza; Glyptocephalus cynoglossus; Erethizon; Nyctereutes; Trachemys; Stenotomus chrysops; Zosteropidae; Strix uralensis; Hippotragus; Vidua paradisaea; Cebinae; Phascolarctos cinereus; Leiocephalus; Carollia perspicillata; Milvus milvus; Cynomys; Psammomys obesus; Sylvia atricapilla; Python regius; Pogona barbata; Aquila heliaca; Eurypygidae; Jacanidae; Lissemys punctata; Ecsenius; Agapornis; Mimus polyglottos; Canis aureus; Tiliqua scincoides; Sebastes mystinus; Sebastes paucispinis; Pomatomus saltatrix; Ariopsis felis; Abronia anzuetoi; Eudyptes chrysocome; Pomacentrus coelestis; Terrapene; Lampropeltis; Embiotoca jacksoni; Geronticus eremita; Fromia indica; Ducula bicolor; Tockus nasutus; Rhinoptera bonasus; Probosciger aterrimus; Monacanthidae; Halichoeres trimaculatus; Phyllopteryx taeniolatus; Cyanocompsa brissonii; Tringa totanus; Chloropsis; Tockus alboterminatus; Tockus deckeni; Chamaeleo calyptratus; Gymnothorax moringa; Centropristis striata; Erpeton; Laemanctus; Labroides bicolor; Cuora mccordi; Amazona agilis; Histrio histrio; Zenopsis conchifer; Uraeginthus bengalus; Bathymaster signatus; Pseudobalistes fuscus; Trachemys scripta scripta; Sebastes borealis; Lutjanus quinquelineatus; Lepidopsetta polyxystra; Oxycheilinus digramma; Giraffa giraffa; Pleoticus muelleri; Ovis orientalis; Geopelia placida; Photoblepharon palpebratum; Calyptocephallela gayi; Scolopsis bilineata; Atherinomorus vaigiensis; Cheilopogon pinnatibarbatus californicus; Leptoclinus maculatus; Coris caudimacula; Gadus chalcogrammus; Doryteuthis pealeii; Crocodylia; Ophioderma panamensis; Notamacropus rufogriseus; Cirrhilabrus lineatus; Illex illecebrosus; Strongylocentrotus purpuratus; Branchiostoma floridae; Galeocerdo cuvier; Callorhinchus milii; Clupea harengus; Salvelinus alpinus; Mya arenaria; Loligo vulgaris; Strongylocentrotus droebachiensis; Holothuria; Ciona intestinalis; Leucoraja erinacea; Lophius piscatorius; Hemitripterus americanus; Cyclopterus lumpus; Thunnus albacares; Testudinidae; Varanus; Gekkonidae; Boa constrictor; Struthio camelus; Sturnus vulgaris; Phoenicopteriformes; Ara; Ara ararauna; Aptenodytes patagonicus; Petauridae; Dasypodidae; Scandentia; Varecia; Saguinus; Macaca sylvanus; Papio hamadryas; Theropithecus gelada; Canis lupus familiaris; Nasua; Martes foina; Mustela putorius; Felis silvestris; Phocidae; Equus; Equus zebra; Sus scrofa; Bison bonasus; Capra; Apodemus sylvaticus; Lagostomus maximus; Myocastor coypus; Saccoglossus kowalevskii; Psittacus; Castoridae; Styela montereyensis; Ardea; Buteo; Buteo buteo; Balearica pavonina; Grus japonensis; Corvus; Bubo bubo; Carcharias taurus; Axis axis; Vicugna; Hippoglossoides elassodon; Trachemys scripta elegans; Leptoptilos crumeniferus; Gypaetus; Morone saxatilis; Hippoglossoides platessoides; Capromys pilorides; Petaurus breviceps; Suricata; Hemitragus; Chloris chloris; Lepas anatifera; Chamaeleonidae; Lutjanus mahogoni; Circus cyaneus; Pithecia pithecia; Patiria miniata; Geochelone; Cyclura; Apodemus flavicollis; Sciurus vulgaris; Centropomus robalito; Cyclura cornuta; Cornufer guentheri; Antidorcas; Antilope; Kobus leche; Agapornis canus; Agapornis lilianae; Agapornis taranta; Varanus gouldii; Scincidae; Sebastes atrovirens; Sebastes caurinus; Sebastes hopkinsi; Sebastes miniatus; Geoemyda spengleri; Mullus surmuletus; Corucia zebrata; Picus viridis; Nothobranchius furzeri; Fromia; Asio otus; Strix aluco; Trioceros jacksonii; Theloderma; Nectariniidae; Ploceus cucullatus; Spinus spinus; Ctenochaetus striatus; Urophycis tenuis; Caloenas nicobarica; Euplectes; Coracias garrulus; Pisaster giganteus; Pleurogrammus monopterygius; Glyptocephalus zachirus; Clavelina picta; Mungos mungo; Accipiter nisus; Fistularia commersonii; Cygnus cygnus; Anoplopoma fimbria; Uromastyx ocellata; Stichopus chloronotus; Trachyphonus erythrocephalus; Coris gaimard; Pytilia melba; Potamochoerus porcus; Ecteinascidia turbinata; Pachyuromys; Holothuria atra; Sebastes semicinctus; Podothecus accipenserinus; Falco cherrug; Pitta moluccensis; Camelus ferus; Ptilinopus pulchellus; Chiroxiphia pareola; Sphoeroides maculatus; Astrochelys yniphora; Boltenia echinata; Echinarachnius parma; Alitta succinea; Bodianus diana; Cantherhines pardalis; Cheilodipterus quinquelineatus; Tetrastes bonasia; Parapercis xanthozona; Lumpenus lampretaeformis; Pseudanthias ventralis; Xenagama wilmsi; Loweina rara; Coracias cyanogaster; Vanellus armatus; Oxycercichthys veliferus; Onuxodon fowleri; Cirrhilabrus roseafascia; Copsychus malabaricus; Hypanus americanus; Xiphias gladius; Ambystoma mexicanum; Heloderma; Casuarius casuarius; Rhea americana; Anas platyrhynchos; Ciconiidae; Columbidae; Accipiter gentilis; Circus aeruginosus; Acryllium vulturinum; Gallus gallus; Perdix perdix; Phasianus colchicus; Coturnix delegorguei; Spheniscus humboldti; Pteropus; Callithrix jacchus; Saguinus oedipus; Saguinus imperator; Macaca; Colobus polykomos; Pongo; Canis lupus; Panthera leo; Panthera pardus; Puma concolor; Tapirus; Sus scrofa domesticus; Camelus dromedarius; Lama glama; Tragulus javanicus; Capreolus capreolus; Rangifer tarandus; Ovis aries; Kobus; Capricornis; Oryctolagus cuniculus; Spermophilus; Cricetus; Rattus norvegicus; Rattus rattus; Amazona; Lynx lynx; Nymphicus hollandicus; Tinca tinca; Dolichotis patagonum; Crassostrea gigas; Incilius alvarius; Chauna torquata; Rollulus; Capromyidae; Vipera berus; Scopus umbretta; Rupicapra rupicapra; Pythonidae; Pelecanus crispus; Cucumaria frondosa; Coccothraustes; Polychrus marmoratus; Cygnus melancoryphus; Erythrura; Phodopus campbelli; Neoniphon sammara; Eunectes; Haliaeetus leucocephalus; Cariamidae; Macaca silenus; Musophagidae; Garrulus glandarius; Leontopithecus chrysomelas; Upupa epops; Paralichthys dentatus; Nanger dama; Myoxocephalus octodecemspinosus; Tragelaphus spekii; Sebastes ovalis; Hypselecara coryphaenoides; Spatula querquedula; Equus asinus asinus; Elephas maximus indicus; Falco tinnunculus; Tetrao urogallus; Testudo kleinmanni; Hoplobatrachus tigerinus; Musophaga; Osteoglossum bicirrhosum; Ptilinopus; Athene noctua; Polypedates otilophus; Correlophus ciliatus; Rhinogobiops nicholsii; Otaria; Leucoraja ocellata; Pycnonotus barbatus; Psarisomus dalhousiae; Cynoscion regalis; Acanthurus triostegus; Alectis ciliaris; Lethrinus atkinsoni; Hippoglossina oblonga; Scophthalmus aquosus; Gallicolumba; Amandava subflava; Furcifer pardalis; Choerodon fasciatus; Coronella austriaca; Thyonella gemmata; Neurergus; Diodon hystrix; Canis lupus lycaon; Euplectes orix; Chromis punctipinnis; Haemulon flavolineatum; Semicossyphus pulcher; Dinemellia; Aplonis panayensis; Hemisphaeriodon; Halocynthia pyriformis; Phloeomys; Cuora mouhotii; Merops apiaster; Pseudanthias; Ambystoma andersoni; Malacochersus; Cyanoliseus patagonus; Ostorhinchus aureus; Zaprora silenus; Platax teira; Saimiriinae; Pseudomonacanthus peroni; Sebastes norvegicus; Dracaena guianensis; Aonyx cinereus; Merops bullockoides; Ammodytes hexapterus; Sufflamen chrysopterum; Cyclopsitta diophthalma; Centropyge heraldi; Parupeneus spilurus; Vermilingua; Folivora; Lethenteron camtschaticum; Callocephalon fimbriatum; Ophiopteris papillosa; Ophiothrix spiculata; Rhyticeros narcondami; Ostorhinchus rueppellii; Octopus vulgaris; Lytechinus variegatus; Squalus acanthias; Mustelus canis; Cyprinus carpio; Salmo salar; Salmo trutta; Pollachius virens; Zoarces americanus; Ambystoma; Iguanidae; Tiliqua rugosa; Natrix tessellata; Crotalus; Dendrocygna viduata; Charadriidae; Ciconia ciconia; Gallus; Coturnix coturnix; Parus major; Sarcophilus; Macropus; Tupaia; Lemur; Papio; Ailurus fulgens; Mustelidae; Lutra lutra; Mustela; Panthera onca; Panthera tigris; Rhinocerotidae; Cervus elaphus; Capra aegagrus; Connochaetes; Lepus europaeus; Marmota; Acomys; Mus musculus; Hystricidae; Melopsittacus; Tamias; Molgula citrina; Botryllus schlosseri; Heleophrynidae; Dama dama; Yangochiroptera; Leontopithecus; Pelecanus; Hippotragus equinus; Ostrea edulis; Cricetomyinae; Uromastyx; Cynictis; Glis glis; Oplurus; Bothriechis schlegelii; Brachylophus; Passer domesticus; Jaculus; Sauromalus; Python molurus; Acanthosaura; Shinisaurus crocodilurus; Plegadis falcinellus; Eliomys quercinus; Corvus corax; Coliiformes; Agapornis personatus; Loriculus galgulus; Leptailurus; Lepus timidus; Astrochelys radiata; Tragelaphus angasii; Sebastes constellatus; Sebastolobus alascanus; Paracanthurus hepatus; Corvus frugilegus; Dascyllus aruanus; Coryphaenoides acrolepis; Testudo hermanni; Paracirrhites forsteri; Scyliorhinus retifer; Nardoa novaecaledoniae; Chaetodon lineolatus; Chaetodon lunula; Buteo lagopus; Batoidea; Loweina terminata; Penaeus; Caiman yacare; Cacatua alba; Paroedura picta; Rhacophorus reinwardtii; Recurvirostra avosetta; Irena puella; Bycanistes bucinator; Elops affinis; Philomachus; Zamenis longissimus; Ascidiella aspersa; Tamiops; Amblyglyphidodon leucogaster; Rhinecanthus aculeatus; Padda oryzivora; Hemilepidotus jordani; Triglops scepticus; Oxylebius pictus; Tockus flavirostris; Taurotragus; Cephalopholis miniata; Aotidae; Sebastes chrysomelas; Pterocaesio marri; Notamacropus parma; Lamprotornis chalcurus; Boltenia ovifera; Rhabdamia gracilis; Chrysopelea; Pristigenys alta; Salvelinus umbla; Holothuria cinerascens; Grus paradisea; Lyrurus tetrix; Ammodytes dubius; Cryptacanthodes maculatus; Prionotus carolinus; Ostorhinchus moluccensis; Apostichopus parvimensis

Type:

Methylation profiling by high throughput sequencing

580 related Platforms

3023 Samples

Download data: BED

(Submitter supplied) XBP transcription factors are well-known regulators of the the unfolded protein response and are required for plasma cell differentiation. However, recent studies have shown that the XBP1 gene is also expressed in the developing notochord of Ciona and various vertebrates, and to date its role in the formation of this organ is largely uncharacterized. We identified putative targets of Ci-XBP1 through a microarray screen, using RNAs extracted from embryos expressing mutant forms of this transcriptional regulator in the notochord. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by array

Platform:

GPL15657

9 Samples

Download data: CEL

(Submitter supplied) Purpose: Understand how the misexpression of key sensory cell determinant POU IV affects cell type specification of various sensory neurons in the peripheral nervous system in Ciona. Methods: CesA>POU IV (2.5ng/l) + CesA>GFP (5.0ng/l) injected eggs and control eggs were fertilized side by side, and allowed to develop to the late tailbud stage (13.5h after fertilization at 18°C). For each sample, 120 morphologically normal embryos were used for single cell RNA-seq assays. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29701

2 Samples

Download data: H5

(Submitter supplied) We recently showed HMX is expressed in bipolar tail neurons (BTN) in early embryos of Ciona intestinalis. In order to assess the function of the homeobox transcription factor in this cell fate, we used an overexpression strategy. ciHMX was overexpressed in the epidermis, followed by RNAseq of experimental and control embryos. We then looked for differential expression of BTN fate markers, testing if HMX is able to regulate BTN fate determination.

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27816

6 Samples

Download data: CSV

(Submitter supplied) The hypothalamus coordinates neuroendocrine functions in vertebrates, including circadian rhythm, metabolism, and appetite. To explore its evolutionary origin, we attempt to create integrated transcription/connectome brain maps for swimming tadpoles of the ascidian, Ciona intestinalis, which serves as an approximation of the ancestral proto-vertebrate. This map features several cell types related to different regions of the vertebrate hypothalamus, including coronet cells, magnocellular neurons and the arcuate nucleus. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29701

4 Samples

Download data: H5

(Submitter supplied) We investigated genome folding across the eukaryotic tree of life. We find four general manifestations of genome organization at chromosome-scale that each emerge and disappear repeatedly over the course of evolution. The submission represents chromosome-length Hi-C contact maps, architecture type and homolog separation analyses for 26 species across the tree of life, representing all subphyla of chordates, all 7 extant vertebrate classes, and 7 out of 9 major animal phyla, as well as plants and fungi.

Organism:

Saccharomyces cerevisiae; Drosophila melanogaster; Gallus gallus; Hypsibius dujardini; Lethenteron camtschaticum; Triticum aestivum; Caenorhabditis elegans; Aplysia californica; Aedes aegypti; Culex quinquefasciatus; Homo sapiens; Muntiacus reevesi; Muntiacus muntjak; Strongylocentrotus purpuratus; Ciona intestinalis; Pleurobrachia bachei; Acropora millepora; Python bivittatus; Arachis hypogaea; Agaricus bisporus; Branchiostoma lanceolatum; Xenopus laevis; Notamacropus eugenii; Pygocentrus nattereri; Cristatella mucedo; Clonorchis sinensis; Chiloscyllium punctatum

Type:

Other

30 related Platforms

32 Samples

Download data: BEDPE, FASTA, HIC, VCF, WIG

(Submitter supplied) Tunicate ascidians exhibit metamorphosis that converts tadpole, swimming larva into immotile adult. In ascidian Ciona intestinalis, the mutant tail regression failed (trf) which shows defects in the metamorphosis was previously reported (Nakayama-Ishimura et al., 2009). In the metamorphosis process, trf larvae settle normally with their adhesive papillae, but do not start tail regression, papillae retraction and sensory vesicle retraction, while development of adult organs proceed. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by array

Platform:

GPL5576

8 Samples

Download data: TXT

(Submitter supplied) Studies of ascidian (sea squirt) embryos have highlighted the importance of cell lineages in animal development for over 100 years. As simple proto-vertebrates, they are also used to explore the evolutionary origins of novel cell types, such as cranial placodes and neural crest in vertebrates. To build upon these efforts we have determined comprehensive single cell transcriptomes of Ciona intestinalis throughout embryogenesis. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

23 Samples

Download data: H5

(Submitter supplied) We applied single cell RNA seq with 10x genomics platform to study co-expression of both Ptf1a and Meis leading to the wholesale transformation of the entire CNS into Coronet cells. Our study identify a cocktail of regulatory determinants for the efficient specification of Coronet cells.

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

4 Samples

Download data: CLOUPE, H5

(Submitter supplied) We sequenced at mRNA level in adult hearts of zebrafish, pamprey and sea squirt. Combined with other 11 vertebrate heart RNA-Seq data online, we conducted comprehensive evolutionary genomic analyses to address the contribution of gene/genome duplications on heart structure evolution. We observed that number of duplicate genes expressed in heart increased gradually with the increase of heart chamber number along the vertebrate phylogeny, despite that most of them were duplicated at the time near to the origin of vertebrates or more ancient. more...

Organism:

Ciona intestinalis; Petromyzon marinus; Danio rerio

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL14875 GPL20963 GPL20962

3 Samples

Download data: FPKM_TRACKING

(Submitter supplied) We applied single cell RNA seq with 10x genomics platform to study the cell identity change in Ciona late tailbud embryos. By comparing single cell data from Pax3/7>Foxc amd control embryos, we found ectopic expression of FoxC in BTN cells transformed into PSC cells, which suggest common shared origin of BTNs and PSCs.

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

2 Samples

Download data: CLOUPE, H5

(Submitter supplied) This dataset provides gene expression levels of single cell and pooled embryos as part of a study on zygotic genome activation in Ciona embryogenesis. The single cell RNA-seq data suggested that Cyclin B3 was a key mediator of this process. This was confirmed by performing RNA-seq on pooled RNA isolated from embryos where Cyclin B3 was knocked down (using morpholinos) or overexpressed (using mRNA injections).

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

105 Samples

Download data: TXT

(Submitter supplied) The key notochord transcription factor Brachyury was ectopically expressed in Ciona embryos under the control of the FoxAa cis-regulatory region (which drives expression in neural, endodermal and mesenchymal lineages in addition to notochord). Misexpression of Brachyury induced 925 genes compared to a control reporter plasmid (Bra>GFP). There was only modest overlap with a set of notochord-enriched genes previously identified by RNAseq of flow-sorted notochord cells, indicating that Brachyury is not a notochord master regulator gene as strictly defined.

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

6 Samples

Download data: TXT

(Submitter supplied) Cleavage and morphogenesis of Ciona embryos was blocked by treatment with cytochalasin B prior to gastrulation. Drug treated embryos and DMSO treated controls were harvested at stage 19.5 (after control embryos had undergone neurulation and were completing notochord intercalation). Only 55 genes showed a significant difference in expression between the two treatments.

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

6 Samples

Download data: TXT

(Submitter supplied) The notochord is the eponymous feature of the chordates and an essential organ in chordate development. The notochord of the invertebrate chordate Ciona consists of only 40 cells, and is a longstanding model for studying differentiation and morphogenesis in a small, simple embryo. Here we perform RNAseq analysis on flow-sorted notochord cells from multiple stages of development to define a comprehensive Ciona notochord transcriptome. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23102

12 Samples

Download data: TXT

(Submitter supplied) DNA binding profiles of three maternal factors (Tcf7, Gata.a and Zic-r.a) in chordate 16-cell stage embryo. 16-cell stage ciona intestinalis embryos were collected from multiple batches and the extracted chromatin was immunoprecipitated by the antibodies which specifically recognize ciona Gata.a, Tcf7 and Zic-r.a, respectively.

Organism:

Ciona intestinalis

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL8993

3 Samples

Download data: TXT

(Submitter supplied) Microarray analysis indicated many changes in gene expression, including genes related the the Notch signaling system, during oral siphon regeneration in adult Ciona. Subsequent qPCR gene expression and inhibitor of experiments confirm a role of the Notch system, probably in the formation of a regeneration blastema.

Organism:

Ciona intestinalis

Type:

Expression profiling by array

Platform:

GPL5576

8 Samples

Download data: TXT

(Submitter supplied) In vertebrates, pluripotent pharyngeal mesoderm progenitors produce the cardiac precursors of the second heart field as well as the branchiomeric head muscles and associated stem cells. However, the cellular and molecular mechanisms underlying the transition from multipotent progenitors to distinct heart and muscle precursors remain obscured by the complexity of vertebrate embryos. Here, using the ascidian Ciona intestinalis as a simple chordate model for cardiopharyngeal development, we show that bipotent progenitors are transcriptionally primed to activate both heart and pharyngeal muscle regulatory programs, which become restricted to the corresponding precursors following a conserved pattern of asymmetric divisions. more...

Organism:

Ciona intestinalis

Type:

Expression profiling by array

Platform:

GPL15657

73 Samples

Download data: CEL