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Improved DNA methylation analysis via enrichment of demethylated cells expressing an X-inactivated GFP transgene

(Submitter supplied) The demethylating drug 5-aza-2’-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we utilize a novel method that uses the reactivation of an X-inactivated GFP transgene as a marker to enrich for cells that have undergone drug-induced demethylation. By combining it with microarray gene expression profiling, we demonstrate the method’s utility in identifying genes activated by global DNA demethylation. more...
Organism:
Mus musculus
Type:
Expression profiling by array; Methylation profiling by array
Platform:
GPL1261
6 Samples
Download data
Series
Accession:
GSE4718
ID:
200004718

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