GEO DataSets

(Submitter supplied) Second slide of 2. HGMP printed NIA 15K set, over 2 slides, 17280 spots per slide, including empties, control spots, landmark spots, and duplicate experimental spots. 12 rows and 4 columns of grids, each grid contains 18 rows and 20 columns of spots (format for both slides 1-20 and 21-40). Protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR, primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification. more...

Organism:

Mus musculus

1 Series

10 Samples

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(Submitter supplied) This cDNA clone set was prepared by researchers at the National Institute on Aging. The clone set has been distributed widely and is currently in use by a number of other microarray programmes. The set has the following characteristics: ~ 15,000 'unique' cDNA clones were rearrayed from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary. Up to 50% are derived from novel genes. more...

Organism:

Mus musculus

2 Series

8 Samples

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(Submitter supplied) HGMP printed NIA 15K arrays, printed on 2 slides, 17280 spots per slide, including control spots, empties and landmarks. 2 duplicate spots per slide for each clone. Earlier versions of these slides have already been described by platform entries GPL5457 and GPL5458. However, owing to analysis of a later array print run by a different image analysis programme in this series of experiments, the data files present in a different clone order and it is impossible to sort them to generate the same order. more...

Organism:

Mus musculus

1 Series

18 Samples

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(Submitter supplied) HGMP printed NIA 15K arrays, printed on 2 slides, 17280 spots per slide, including control spots, empties and landmarks. 2 duplicate spots per slide for each clone. This is the platform submission for Slide 1 Protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR, primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification. more...

Organism:

Mus musculus

1 Series

10 Samples

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(Submitter supplied) cDNA array created by amplification of the National Institute of Aging 15K clone set. All clones were PCR amplified using M13 forward and reverse primers, cleaned with millipore filter plates and printed in 3xSSC and 1.5M betaine with a Genomic Solutions OmniGrid 100 robot. Arrays were printed on Corning GAPSII amino coated slides. 32 Spalax sequences were added to the array.

Organism:

Mus musculus; Nannospalax ehrenbergi

1 Series

12 Samples

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(Submitter supplied) Custom array including the NIA Mouse 15K Cloneset plus addition unidentified clones Keywords = Mouse NIA 15K Arizona

Organism:

Mus musculus

2 DataSets

2 Series

24 Samples

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(Submitter supplied) The objective of the present study was to use our own fabricated bovine embryo cDNA microarray to profile genome-wide expression patterns of genes during the implantation period. Duplicate readings were obtained by hybridization of a Cy3/Cy5 probe mixture for each sample. Data normalization was performed the local background intensity of each array spot was smoothed by the local weight regression (lowess) smoother and subtracted from spot intensity data. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS1003

Platform:

GPL1221

11 Samples

Download data: TIFF

Expression profiling of embryos at various days of gestation up to 21 days and fetuses and fetal membranes at 27 and 28 days of gestation. Results provide insight into the regulation of embryonic, fetal, and fetal membrane differentiation.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 7 development stage, 3 tissue sets

Platform:

GPL1221

Series:

GSE1414

11 Samples

Download data: TIFF

(Submitter supplied) In early embryos, DNA methylation is remodelled to initiate the developmental program. For mostly unknown reasons, methylation marks are acquired unequally between embryonic and placental cells. To better understand this, we generated high-resolution maps of DNA methylation in mouse mid-gestation (E10.5) embryo and placenta. We uncovered specific subtypes of differentially methylated regions (DMRs) that contribute directly to the developmental asymmetry existing between mid-gestation embryo and placenta. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) BPL_array_NIAS platform of a spotted cDNA microarray has been established using a bovine utero-placenta cDNA library. The array has been fabricated collaboratively by Laboratory of Reproductive Biology and Technology at National Institute of Agrobiological Sciences, Tsukuba City, Japan and Department of Paediatric Pharmacology at National Institute of Child Health and Development, Tokyo, Japan. Keywords = bovine utero-placenta Keywords = cDNA array Keywords = gene expression

Organism:

Bos taurus

1 DataSet

2 Series

29 Samples

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(Submitter supplied) Gene expression patterns of bovine placenta from initial to late stage of pregnancy were investigated by using the our custom designed utero-placental cDNA microarray. Keywords: Time course

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL1221

18 Samples

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(Submitter supplied) E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used to assess the accuracy and precision of the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL870

6 Samples

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(Submitter supplied) E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was used to assess the false-positive rates in the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL870

6 Samples

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(Submitter supplied) E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RNA, and one round of amplification was performed with 250 ng and 50 ng total RNA inputs. For 10 ng and 2 ng inputs, two rounds of amplification were used. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL870

8 Samples

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(Submitter supplied) cDNA microarrays have been shown to be useful for monitoring global gene expression patterns in normal and disease states and in response to various environmental stimuli. In this study we have used a cattle cDNA microarray containing 7653 elements to analyze expression profiles in 19 different cattle tissues. Signal intensities from all tissue sample RNAs were compared to a reference standard RNA created from different tissues and cell lines. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2672

39 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

86 Samples

Download data: CEL

(Submitter supplied) We used full genome microarrays to profile the full lifetime of the mouse placenta from embryonic day 8.5 (e8.5), at the time of chorioallantoic fusion, until postnatal day 0 (P0). For these samples, at each stage the fetal placenta and maternal decidual tissues were dissected and profiled separately (See series 1). For this experiment (Series 2), placental and decidual timecourse samples were normalized and modeled with two undissected (including placental and decidual tissue) e17 placentas to allow for scaling of values for comparison to the undissected placenta samples used in the publicly available mouse GeneAtlas dataset Keywords: time course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

42 Samples

Download data: CEL

(Submitter supplied) We used full genome microarrays to profile the full lifetime of the mouse placenta from embryonic day 8.5 (e8.5), at the time of chorioallantoic fusion, until postnatal day 0 (P0). At each stage, the fetal placenta and maternal decidual tissues were dissected and profiled separately Keywords: time course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

44 Samples

Download data: CEL