GEO DataSets

Analysis of 4 breast cell lines treated for up to 36 hrs with the chemotherapeutic agents 5-fluorouracil (5FU), doxorubicin (DOX), or etoposide (ETOP), a drug mechanistically similar to DOX. Expression profiles for DOX- and 5FU-treated cells were used to successfully predict the response to ETOP.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 agent, 4 cell line, 3 time sets

Platform:

GPL550

Series:

GSE1647

83 Samples

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(Submitter supplied) Global gene expression profiling has demonstrated that the predominant cellular response to a range of toxicants is a general stress response. This stereotyped environmental stress response commonly includes repression of protein synthesis and cell cycle regulated genes and induction of DNA damage and oxidative stress responsive genes. Our laboratory has recently characterized the general stress response of breast cell lines derived from basal-like and luminal epithelium following treatment with doxorubicin (DOX) or 5-fluorouracil (5FU) and showed that each cell type has a distinct response. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1627

Platform:

GPL550

83 Samples

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(Submitter supplied) Recent studies have identified clinical subtypes of breast tumors that arise from different cell types and have different outcomes in response to chemotherapy. Tumors derived from basal epithelium have a poorer prognosis than tumors derived from luminal epithelium. To gain insight into differences underlying this disparity, we treated cell lines derived from basal epithelium (immortalized human mammary epithelial cells) and those derived from luminal epithelium (MCF-7 and ZR-75-1) with two chemotherapeutics commonly used in the treatment of breast cancer. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS845 GDS846 GDS847 GDS848

Platform:

GPL550

75 Samples

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Analysis of the response of luminal epithelium derived breast cancer line ZR-75-1 to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

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Analysis of the response of luminal epithelium derived breast cancer line MCF-7 to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

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Analysis of the response of basal epithelium derived breast cancer line HME-CC to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

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Analysis of the response of the basal epithelium derived breast cancer line ME16C to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

18 Samples

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(Submitter supplied) Tumor cell lines and drug-resistant counterparts. These data support the publication Gyorffy et al, Oncogene 2005 (July), Prediction of doxorubicin sensitivity in breast tumors based on gene expression profiles of drug-resistant cell lines correlates with patient survival. We contrasted the expression profiles of 13 different human tumor cell lines of gastric (EPG85-257), pancreatic (EPP85-181), colon (HT29) and breast (MCF7 and MDA-MB-231) origin and their counterparts resistant to the topoisomerase inhibitors daunorubicin, doxorubicin or mitoxantrone. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL2706 GPL2705

13 Samples

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(Submitter supplied) Spotted cDNA Orestes clones. This set includes 4608 orestes in build 175 of the human UniGene database. Keywords = spotted, cDNA

Organism:

Homo sapiens

13 Series

1103 Samples

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(Submitter supplied) Spotted cDNA Orestes clones and PCR amplified fragments on glass. This set includes 664 orestes and 197 PCR amplified fragments based upon representative sequences in build 175 of the human UniGene database. Keywords = spotted Keywords = cDNA

Organism:

Homo sapiens

1 Series

118 Samples

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(Submitter supplied) Genome wide DNA methylation profiling of locally advanced breast tumors before and after treatment with doxorubicin and 5-fluorouracil Mitomycin C

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL8490

61 Samples

Download data: IDAT

(Submitter supplied) Breast cancer subtypes identified in genomic studies have different underlying genetic defects. Mutations in the tumor suppressor p53 occur more frequently in estrogen receptor (ER) negative, basal-like and HER2-amplified tumors than in luminal, ER positive tumors. Thus, because p53 mutation status is tightly linked to other characteristics of prognostic importance, it is difficult to identify p53's independent prognostic effects. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL885 GPL887

48 Samples

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(Submitter supplied) Anti-cancer drug testing is challenging, but genetically engineered mouse models (GEMMs) and orthotopic, syngeneic transplants (OSTs) may offer advantages for pre-clinical testing including an intact microenvironment. We examined the efficacy of six chemotherapeutic or targeted anti-cancer drugs, alone and in combination, using over 500 GEMMs/OSTs representing three distinct breast cancer subtypes: Basal-like (C3(1)-T-antigen GEMM), Luminal B (MMTV-Neu GEMM), and Claudin-low (T11/TP53-/- OST). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

19 Samples

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(Submitter supplied) We use high-density tiling DNA microarray technology to obtain the specific transcriptome-wide response induced by 5-Fluorouracil (5FU) in the eukaryotic model Schizosaccharomyces pombe.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by genome tiling array

Platform:

GPL7715

4 Samples

Download data: CEL, WIG

(Submitter supplied) We generated RNA's and measured expression of 40000 genes using spotted cDNA microarrays from the fifty nine publicly available cell lines of the NCI Developmental Therapeutics Program's NCI60 studies and an additional set of seven cell lines for which GI50 compound sensitivity data were available. All cell lines were grown to 80% confluence in RPMI 1640 supplemented with phenol red, glutamine (2 mM) and 5% fetal calf serum. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3043 GPL3417

66 Samples

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(Submitter supplied) Cell lines derived from human tumors have historically served as the primary experimental model system for exploration of tumor cell biology and pharmacology. Cell line studies, however, must be interpreted in the context of artifacts introduced by selection and establishment of cell lines in vitro. This complication has led to difficulty in the extrapolation of biology observed in cell lines to tumor biology in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

8 related Platforms

24 Samples

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(Submitter supplied) Purpose: The goals of this study are to investigate how the transcriptome of colon cancer cells responds to genotoxic 5FU treatments utilizing single cell RNA-seq (scRNA-seq) technology. Methods: The collection of the single cell transcriptomes obtained from three colon cancer cell lines, RKO (CRL-2577), HCT116 (CCL-247) and SW480 (CCL-228), was generated by Drop-seq method followed by sequencing through Illumina HiSeq-4000 High-Output system. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

10 Samples

Download data: CSV, TXT