GEO DataSets

(Submitter supplied) SAGE libraries generated from total testes of adult mouse males. Keywords = testis Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) APOE4 allele is a major risk factor for late-onset Alzheimer disease (AD). The mechanism of action of APOE in AD remains unclear. To study the effects of APOE alleles on gene expression in AD, we have analyzed the gene transcription patterns of human hippocampus from APOE3/3, APOE3/4, APOE4/4 AD patients and normal control using Serial Analysis of Gene Expression (SAGE). Using SAGE, we found gene expression patterns in hippocampus of APOE3/4 and APOE4/4 AD patients differ substantially from those of APOE3/3 AD patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL4 GPL1485

6 Samples

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(Submitter supplied) Gene expression profiles of somatic cells of fetal and adult testis. To identify genes developmentally regulated in the somatic cells of the testis, serial analysis of gene expression (SAGE) has been used to generate gene expression profiles from these cells in the fetal and adult mouse. To avoid germ cell transcripts, a fetal SAGE library was generated from germ cell-free fetal Wv/Wv mice, and an adult SAGE library was generated from adult testes depleted of germ cells with busulfan. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) The analysis of differentially expressed genes is a powerful approach to elucidate the genetic mechanisms underlying the morphological and evolutionary diversity among serially homologous structures, both within the same organism (e.g., hand vs. foot) and between different species (e.g., hand vs. wing). In the developing embryo, limb-specific expression of Pitx1, Tbx4, and Tbx5 regulates the determination of limb identity. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) Forty male C57BL6 mice 12-15 weeks old fed chow ad libitum. 30 mice underwent adrenalectomy (Adx) and 10 others a sham-Adx. Three or 24 h before tissue sampling, groups of 10 mice received an I.P. injection of cortisol (0.1 mg per mice), while the remaining Adx and intact mice received an I.P. injection of the vehicle (ethanol) 24 h prior to the sacrifice. All mice were killed within a period of a few hours. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

4 Samples

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(Submitter supplied) Forty male C57BL6 mice 12-15 weeks old fed chow ad libitum. 30 mice underwent adrenalectomy (Adx) and 10 others a sham-Adx. Three or 24h before tissue sampling, groups of 10 mice received an I.P. injection of cortisol (0.1 mg per mice), while the remaining Adx and intact mice received an I.P. injection of the vehicle (ethanol) 24 h prior to the sacrifice. All mice were killed within a period of a few hours. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1862

Platform:

GPL81

4 Samples

Download data: CEL, EXP

Expression profiling of gastrocnemius muscle of adrenalectomized C57BL6 males at 3 and 24 hours following intraperitoneal injection of cortisol. Results compared with those from SAGE (GSE2122) to assess the reproducibility of SAGE experiments.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 protocol, 3 time sets

Platform:

GPL81

Series:

GSE2121

4 Samples

Download data: CEL, EXP

(Submitter supplied) We performed mRNA sequencing of samples isolated from the heads, thoraxes, and abdomens of males and females of Drosophila willistoni to identify genes that are differentially expressed between the sexes.

Organism:

Drosophila willistoni

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL14358 GPL14359

8 Samples

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(Submitter supplied) Genes with sex-biased expression in adults experience unique evolutionary dynamics. It is unclear, however, whether the selection pressures responsible for these well documented patterns also act upon genes with sex-biased expression in other developmental stages. To examine this, we measured expression in male and female Drosophila melanogaster larvae.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6385

8 Samples

Download data: TXT

(Submitter supplied) Investigation of sex-biased expression across species have relied on measurements from whole flies which sample the extensive expression differences found in the germline and gonads of females and males. We wanted to examine genes with sex-biased expression in a somatic tissue to analyze patterns of genes with sex-biased expression in the context of a tissue more phenotypically similar between females and males. more...

Organism:

Drosophila pseudoobscura; Drosophila mojavensis; Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL4631 GPL4629 GPL4630

24 Samples

Download data: FTR, PAIR, TXT

(Submitter supplied) Background - Mammalians gamete production takes place in the testis but when they exit this organ, although spermatozoa have a specialized and distinct morphology, they are immotile and infertile. It is only after their travel in the epididymis that sperm acquire their motility and fertility. Epididymis can be divided in three gross morphological regions, head (caput), body (corpus) and tail (cauda), containing a long and unique convoluted tubule connecting the testis to the vas deferens. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3729

96 Samples

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(Submitter supplied) Identification and annotation of all the genes in the sequenced Drosophila genome is a work in progress. Wild-type testis function requires many genes and is thus of potentially high value for the identification of transcription units. We therefore undertook a survey of the repertoire of genes expressed in the Drosophila testis by computational and microarray analysis. We generated 3141 high-quality testis expressed sequence tags (ESTs). more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5

8 Samples

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(Submitter supplied) The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). more...

Organism:

Drosophila melanogaster

1 Series

8 Samples

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(Submitter supplied) Goal: To identify the genes associated with the decision of undifferentiated spermatogonia to commit to a pathway of differentiation. Methods: testis mRNA profiles of 10 juvenile wild-type rhesus monkeys (3 vehicle-treated; 6 gonadotropin treated) were generated by deep sequencing, in triplicate and quadruplicate, using Illumina NextSeq500. The sequence reads that passed filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21120

10 Samples

Download data: BEDGRAPH, FA, GTF, TXT

(Submitter supplied) Expression profiles were analyzed of mouse testis in four age groups (3, 6, 12, 18 postnatal month) by total RNA sequencing.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

12 Samples

Download data: GTF, TXT

(Submitter supplied) 14 bp RsaI SAGE analysis of testes from 3 month and 29 month BDF1 mice, and 14 month SAMP1 mice. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL923

3 Samples

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(Submitter supplied) SAGE and MPSS libraries were produced from the same RNA sample extracted from an activated CD4+ T cell clone in order to compare the ability of these techniques to indentify the full range of genes expressed in a single cell type. Keywords: Technical comparison of tag-based technologies SAGE and MPSS

Organism:

Homo sapiens

Type:

Expression profiling by SAGE; Expression profiling by MPSS

Platforms:

GPL5624 GPL5678

4 Samples

Download data: TXT

(Submitter supplied) We conducted a genome-wide expression analysis of wild-type males using three cell populations isolated from mitotic, meiotic and post-meiotic phases of spermatogenesis in Drosophila melanogaster. Our approach was to directly isolate testis regions enriched with RNAs from each of the three specific germline phases. We used microarrays to detail the global gene expression profile in spermatogenesis and identified up- and down-regulated genes between two different spermatogenic phases in pairwise comparisons

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

9 Samples

Download data: CEL

(Submitter supplied) We used tiling arrays to compare the testicular expression of chromosomes 2, X, Y and the mitochondrial genome between Mus m. domesticus and Mus spretus. We found an excess of dysregulated non-coding RNAs on chromosome 2. This group consists of intronic antisense RNAs, intergenic RNAs and also premature forms of PIWI-interacting RNAs (piRNAs). The unique transcriptional regulation in spermatogenesis is a characteristic of chromosome X. more...

Organism:

Mus musculus; Mus musculus domesticus; Mus spretus

Type:

Expression profiling by genome tiling array

Platform:

GPL6445

6 Samples

Download data: BED, CEL

(Submitter supplied) Trout spermatogenesis proceeds seasonally in a way that all morphological and cellular events tend to be synchronized. We thus used gonads at key stages of the male reproductive cycle together with isolated germ cell populations to study the changes in gene expression underlying testis development. Statistical analysis followed by clustering of expression profiles allowed the discrimination of sequential events of gene activation or repression during testis development and/or throughout the germline.

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL3650

38 Samples

Download data: QTF