GEO DataSets

(Submitter supplied) Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244,313 SAGE tags from transcripts expressed in Drosophila embryonic, larval, pupae, adult, and testicular tissue. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by SAGE

Platform:

GPL1882

5 Samples

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(Submitter supplied) Rice blast, caused by the fungal pathogen Magnaporthe grisea, is a devastating disease causing tremendous yield loss in rice production. The public availability of the complete genome sequence of M. grisea provides ample opportunities to understand the molecular mechanism of its pathogenesis on rice plants at the transcriptome level. To identify all the expressed genes encoded in the fungal genome, we have analyzed the mycelium and appressorium transcriptomes using MPSS, RL-SAGE and oligoarray methods. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL4080 GPL4085

2 Samples

Download data: TXT

(Submitter supplied) Triticum aestivum cultivars Scorpion 25 and Xi 19 were grown under both normal and hot/dry conditions. We compared the effect of these two growth conditions on these two closely related varieties.

Organism:

Triticum aestivum

Type:

Expression profiling by SAGE

Platform:

GPL7079

6 Samples

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(Submitter supplied) A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

1 Sample

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(Submitter supplied) These libraries represent total RNA from bursal tissue of 20 day old CB-inbred chicks and the ALV induced bursal cell line DT40 Cre1 cells. Keywords = chicken transcriptome SAGE Bursal cell DT40 Keywords: other

Organism:

Gallus gallus

Type:

Expression profiling by SAGE

Platform:

GPL1477

2 Samples

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(Submitter supplied) SAGE libraries from mouse

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

5 Samples

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(Submitter supplied) This library was generated from normal human thyroid tissue collected at routine autopsy. Standard SAGE protocol was used (Velculescu et al. Science 1995). Data from this library was published in: 'Pauws E, Moreno JC, Tijssen M, Baas F, de Vijlder JJ, Ris-Stalpers C. J Clin Endocrinol Metab. 2000 May;85(5):1923-7' Data was generated and analyzed at the laboratory of Pediatric Endocrinology, Academic Medial Center, Amsterdam, The Netherlands.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) Evaluation of extraocular muscle (EOM) critical period by expression profiling in dark reared rat. Data form part of publication: Investigative Ophthalmology and Visual Sciences 44: 3842-3855, 2003. Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

2 Samples

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(Submitter supplied) Many animal and plant genomes are transcribed much more extensively than current annotations predict. However, the biological function of these unannotated transcribed regions is largely unknown. To begin to address this question, we have generated a series of transcription maps at an interrogation resolution of 35 base pairs (bp) across the Drosophila genome for the first 24 hrs of development in 2 hour increments, similar to maps we have created for regions of the human genome. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL3923

12 Samples

Download data: CEL

(Submitter supplied) PURPOSE: To compare the gene expression profiles of normal human corneal endothelium with Fuchs' corneal endothelium, by using serial analysis of gene expression (SAGE). METHODS: Three pairs of normal human corneas were obtained from eye banks. Thirteen bisected Fuchs' corneal buttons were processed at the time of corneal transplantation. The endothelia of normal and Fuchs'-affected corneas were stripped, and total RNA was isolated. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) Reticulocytes were purified from fresh blood samples obtained from 10 healthy adult volunteers (5 women and 5 men) after informed consent. The donors had normal blood cell counts, blood smears, hemoglobin electrophoresis, red cells membrane resistance tests and no biological evidence of hemolysis. Whole blood samples were centrifuged, the supernatant and the buffy coat containing white blood cells (WBC) and platelets were removed. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL6

1 Sample

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(Submitter supplied) Arabidopsis thaliana (Col-4) aerial tissue collected from 14-day old plants. Grown under sterile conditions at 22 degrees Celsius and 125 uE light on 0.5xMS media.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by SAGE

Platform:

GPL25

1 Sample

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(Submitter supplied) SAGE analysis of genes expressed in rat brain microvessels. Keywords: Novel gene identification

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) We used high throughput EST sequences to characterize and generate expression profiles for all transcripts encoded in the mitochondrial genome of D. melanogaster and D. pseudoobscura. Mapping of ESTs confirmed previously predicted transcripts and revealed non-canonical transcript structures. Expression of mitochondrial transcripts was not different between the two species, but it was significantly different for males and females. more...

Organism:

Drosophila melanogaster; Drosophila pseudoobscura

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL9772 GPL9773

4 Samples

Download data: FNA, QUAL, TXT

(Submitter supplied) Glomeruli were isolated from the kidney of a 72 yo male patient. The kidney was surgically removed due to renal cell carcinoma. The glomeruli were isolated from uninvolved renal parenchyma. Histology (H&E) of glomeruli and interstitium was normal. Keywords: Kidney Subcompartment

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL1485

1 Sample

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(Submitter supplied) We have analyzed the pattern of gene expression in human primary CD34(+) stem/progenitor cells. We identified 42,399 unique serial analysis of gene expression (SAGE) tags among 106,021 SAGE tags collected from 2.5 x 10(6) CD34(+) cells purified from bone marrow. Of these unique SAGE tags, 21,546 matched known expressed sequences, including 3,687 known genes, and 20,854 were novel without a match. The SAGE tags that matched known sequences tended to be at higher levels, whereas the novel SAGE tags tended to be at lower levels. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) SAGE profile of rat hippocampus mRNA from Wistar rats

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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