GEO DataSets

(Submitter supplied) Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3271

6 Samples

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(Submitter supplied) 6 lean humans were submitted to a 3 hours hyperinsulinemic-euglycemic clamp. Skeletal muscle biopsies were taken before and after the clamp. Set of arrays that are part of repeated experiments

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6991

6 Samples

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(Submitter supplied) This study was undertaken to test the hypothesis that short term exposure (4 hours) to physiologic hyperinsulinemia in normal, healthy subjects without a family history of diabetes would induce a low grade inflammatory response, independently of glycemic status. We performed euglycemic hyperinsulinemic (80 mU/m2/min) clamps in 12 healthy, insulin sensitive subjects with no family history of diabetes followed by biopsies of the vastus lateralis muscle taken basally and after 30 and 240 minutes of insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3181

Platform:

GPL96

36 Samples

Download data: CEL

Analysis of skeletal muscles from normal, healthy glucose-tolerant individuals exposed to acute physiological hyperinsulinemia for up to 4 hours. Results suggest a low-grade inflammatory response which may provide insight into the etiology of insulin resistance in type 2 diabetes and obesity.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 12 individual, 3 time sets

Platform:

GPL96

Series:

GSE9105

36 Samples

Download data: CEL

(Submitter supplied) Insulin is a potent pleiotropic hormone that affects processes such as cellular growth, differentiation, apoptosis, ion flux, energy expenditure, and carbohydrate, lipid, and protein metabolism. We used microarrays to detail the global programme of gene expression underlying the influence of insulin in human skeletal muscle collected from different human individuals including 20 insulin sensitive, 20 insulin resistant and 15 diabetic patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3715

Platform:

GPL91

110 Samples

Download data: CEL, TXT

Analysis of vastus lateralis muscle biopsies from insulin-sensitive subjects, insulin-resistant subjects, and diabetic patients, following insulin treatment. Results provide insight into the molecular basis of insulin action in skeletal muscle and the underlying defects causing insulin resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 disease state sets

Platform:

GPL91

Series:

GSE22309

110 Samples

Download data: CEL

(Submitter supplied) Note: GSE4063 and GSE4065 are not directly comparable. This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

24 Samples

Download data: CEL, EXP

(Submitter supplied) Analysis of AMPK gamma3-dependent transcriptional responses by analyzing global gene expression in the white portion of the gastrocnemius muscle in AMPK gamma3 mutant (R225Q) transgenic mice and corresponding wild type littermates. Keywords: Genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1939

Platform:

GPL339

12 Samples

Download data: CEL, EXP

(Submitter supplied) Analysis of AMPK gamma3-dependent transcriptional responses by analyzing global gene expression in the white portion of the gastrocnemius muscle in AMPK gamma3 knock-out mice and corresponding wild type littermates. Keywords: Genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1938

Platform:

GPL339

12 Samples

Download data: CEL, EXP

Analysis of white portions of gastrocnemius muscles from AMP-activated protein kinase (AMPK) gamma-3 R225Q transgenic mutants. AMPK gamma-3 is important in glucose uptake, glycogen synthesis, and fat oxidation. Results compared to those from AMPK gamma-3 knockout mutant (GDS1938).

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE4065

12 Samples

Download data: CEL, EXP

Analysis of white portions of gastrocnemius muscles from AMP-activated protein kinase (AMPK) gamma-3 knockout mutants. AMPK gamma-3 is important in glucose uptake, glycogen synthesis, and fat oxidation. Results compared to those from AMPK gamma-3 R225Q transgenic mutant (GDS1939).

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE4063

12 Samples

Download data: CEL, EXP

(Submitter supplied) We examined global mRNA expression using cDNA microarrays in skeletal muscle of humans before, and 3h and 48h after a single bout of exhaustive endurance exercise (cycling). Keywords: Time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1679

Platform:

GPL3457

8 Samples

Download data: GPR

(Submitter supplied) 8,432 randomly chosen cDNAs from a 40k Research Genetics human cDNA library were amplified under standard conditions (GeneAmp PCR System 9700) with the help of a volumetric robot (Tecan/Genesis, RSP150 and Tomtec/Quadra 384). Amplified products were transferred to a 384-well plates, purified transferred to 384-well print plates (Genetix), dried and resuspended in print buffer (3xSSC, 1.5M Betaine). more...

Organism:

Homo sapiens

2 DataSets

3 Series

15 Samples

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Expression profiling of vastus lateralis muscles 3 and 48 hours after a 75-minute bout of high-intensity cycling. Results identify transcriptional pathways activated in skeletal muscles recovering from endurance exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 time sets

Platform:

GPL3457

Series:

GSE4247

8 Samples

Download data: GPR

(Submitter supplied) Abstract: Thyroid hormones are key regulators of metabolism that modulate transcription via nuclear receptors. Hyperthyroidism is associated with increased metabolic rate, protein breakdown, and weight loss. Although the molecular actions of thyroid hormones have been studied thoroughly, their pleiotropic effects are mediated by complex changes in expression of an unknown number of target genes. Here, we measured patterns of skeletal muscle gene expression in five healthy men treated for 14 days with 75 µg of triiodothyronine, using 24,000 cDNA element microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

24 Samples

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(Submitter supplied) Healthy men received 75 5g/d of T3 for 14 days. Microbiopsies of vastus lateralis skeletal muscle were performed before and after the treatment. For microarray experiments, we used samples from five subjects. After amplification of total RNA (Wang et al. 2000a), fluorescently labeled cDNA was prepared from each experimental sample. For each subject, probes from basal and T3 treatment conditions labeled with cyanine (Cy) 3 or Cy5 dyes were hybridized to cDNA microarray. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2905

5 Samples

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(Submitter supplied) Each tissue or cell line polyA+ RNA-labeled with Cy5 was hybridized against a common reference pool consisting of 11 different cell lines described elsewhere (Perou et al. 2000) Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

19 Samples

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(Submitter supplied) Preterm birth is an important unsolved clinical problem. Despite advanced treatments, infants who survive prematurity remain at increased risk for permanent disabilities. In approximately one-third of cases, prematurity is related to infection and/or inflammation, which renders hostile the normally receptive intrauterine environment. Proinflammatory cytokines provoke up-regulation of genes that promote uterine contractions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

6 Samples

Download data: CEL, CHP

(Submitter supplied) JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 30 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask. JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 60 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1711

Platform:

GPL1945

12 Samples

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(Submitter supplied) Yeast strain lacking the two genes SSA1 and SSA2, which encode cytosolic chaperones, acquires thermotolerance as well as the mild heat-shocked wild-type yeast strain. Keywords: Stress response

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2674

3 Samples

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