GEO DataSets

(Submitter supplied) Regulatory T cells (Treg cells) expressing the forkhead family transcription factor Foxp3 are critical mediators of dominant immune tolerance to self. Most Treg cells constitutively express the high-affinity interleukin 2 (IL-2) receptor alpha-chain (CD25); however, the precise function of IL-2 in Treg cell biology has remained controversial. To directly assess the effect of IL-2 signaling on Treg cell development and function, we analyzed mice containing the Foxp3gfp knock-in allele that were genetically deficient in either IL-2 (Il2-/-) or CD25 (Il2ra-/-). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2061

Platform:

GPL1261

5 Samples

Download data: CEL

Analysis of interleukin 2 (IL-2)-deficient, Foxp3-expressing regulatory T (Treg) cells. Foxp3+ Treg cells mediate dominant immune tolerance to self. Treg cells express IL-2 receptor alpha-chain CD25, but the role of IL-2 has been controversial to date. Results identify a role for IL-2 in Treg cells.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 cell type, 4 genotype/variation sets

Platform:

GPL1261

Series:

GSE4179

5 Samples

Download data: CEL

(Submitter supplied) To clarify how Foxp3 regulates its target genes, we performed co-immunoprecipitation experiments and found that Foxp3 physically bound to AML1/Runx1 (Ono, M. et al, Nature, 2007). In this series of study, we compared gene regulations by AML1, wild type Foxp3, and a Foxp3 mutant with defective binding to AML1. Keywords: Retroviral gene-transduction into primary CD4+ T cells

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) Analysis of Foxp3 ablated peripheral regulatory T cells. Regulatory T cells require the expression of the transcription factor Foxp3 for thymic development. It is not known whether continuous expression of Foxp3 is required for the maintained function of mature regulatory T cells in the periphery. Results indicate changes to the regulatory T cell developmental program in the absence of Foxp3. Keywords: genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2525

Platform:

GPL1261

4 Samples

Download data: CEL

Analysis of mature regulatory T cells (Treg) ablated for the transcription factor Foxp3. Foxp3 is required for the development of Treg cells. Results provide insight into the role of Foxp3 in maintaining the transcriptional and functional program established during Treg cell development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL1261

Series:

GSE6681

4 Samples

Download data: CEL

(Submitter supplied) Examination of CD4+ T cells from Foxp3-GFP knock-in mice. Aim is to understand the genetic program governed by Foxp3 in T cells by comparison of CD4 T cells subdivided into four groups based on expression of Foxp3 and CD25. Keywords = regulatory T cells Keywords = Foxp3 Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1113

Platform:

GPL1261

4 Samples

Download data: CEL

Analysis of CD4+/CD25lo, CD4+/CD25+, and CD4+/CD25hi T cells expressing the forkhead transcription factor Foxp3. FACS used to sort CD4+ cells based on Foxp3 GFP fusion protein and CD25 expression. Results provide insight into the role of Foxp3 in regulatory T cell development and function.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 cell type, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE2389

4 Samples

Download data: CEL

(Submitter supplied) To gain a molecular view of E-proteins with respect to the development of Foxp3+ T cells, we perform microarray studies that would identify transcription factors that are up-regulated as E-proteins levels fall and and Foxp3 expression rises. We hypothesize that such transcription factors activate the synthesis of key proteins necessary for the development of Foxp3+ cells in the thymus (or in the periphery). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

8 Samples

Download data: TXT

(Submitter supplied) High-capacity methods for assessing gene function have become increasingly important because of the increasing number of newly identified genes emerging from large-scale genome sequencing and cDNA cloning efforts. We investigated the use of DNA microarrays to identify uncharacterized genes specifically involved in human T cell activation. Activation of human peripheral blood T lymphocytes induced significant changes in hundreds of transcripts, but most of these were not unique to T cell activation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL771

42 Samples

Download data

(Submitter supplied) Oligonucleotide array on glass. Contains 60-mer probes (60-mer). Submitted for Genomics publication Mao et al. Keywords = Human 50k v1 array

Organism:

Homo sapiens

1 Series

42 Samples

Download data

(Submitter supplied) Gene expression profiles were compared between regulatory T cells (Treg) and Effector CD4+ T cells in healthy B6 mice and sick mice with scurfy mutation. We used microarrays to elucidate the molecular mechanisms underlying the suppression function of the Treg cells. Keywords: Cell type comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Foxp3+ regulatory T cells (Treg cells) maintain immunological tolerance and their deficiency results in fatal multi-organ autoimmunity. Although heightened T cell receptor (TCR) signaling is critical for the differentiation of Treg cells, the role of TCR signaling in Treg cell function remains largely unknown. Here we demonstrate inducible ablation of the TCR results in Treg cell dysfunction which cannot be attributed to impaired Foxp3 expression, decreased expression of Treg cell signature genes or altered ability to sense and consume interleukin 2. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

10 Samples

Download data: CEL

(Submitter supplied) The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4+CD25+ regulatory T cells (TREG), which play a key role in maintaining self tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by the Nuclear Factor of Activated T cells (NFAT). Various mechanisms have been proposed by which FOXP3 mediates these effects. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7716

12 Samples

Download data: TXT

(Submitter supplied) Cytokines critically control immune responses, but how regulatory programs are altered to allow T cells to differentially respond to distinct cytokine stimuli remains poorly understood. Here, we have globally analyzed enhancer elements bound by IL-2-activated STAT5 and IL-21-activated STAT3 in T cells and identified Il2ra as the top-ranked gene regulated by an IL-2-activated STAT5-bound superenhancer and one of the top genes regulated by STAT3-bound superenhancers. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9250 GPL13112

28 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) We previously proposed that lymphocyte homeostasis is achieved by a quorum-sensing like mechanism, based on the paracrine sensing of IL-2 by Foxp3(+) regulatory T CD4(+) cells. In turn, these cells will suppress IL-2 producing cells, thereby controlling the total number of T CD4(+) cells. As CD4(+) T regulatory cells are unable to produce IL-2, such control mechanism assumes the complete segregation of both lymphocyte subsets, that is to say they constitute distinct compartments. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

19 Samples

Download data: TXT

(Submitter supplied) Many species of bacteria use quorum sensing to sense the amounts of secreted metabolites and adapt their growth according to their population density. We asked whether similar mechanisms would operate in lymphocyte homeostasis. We investigated the regulation of the size of Interleukin-2-producing CD4+ T-cell (IL-2p) pool using different IL-2-reporter mice. We found that in the absence of either IL-2 or regulatory CD4+ T-cells (Treg) the number of IL-2p-cells increases. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

10 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL19057

32 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Here we investigated the underlying mechanisms causing the suppression of normal immune responses when T cells are rendered anergic by tolerance induction. By performing an integrated analysis of signaling, epigenetic modifications and gene expression, we demonstrate that immunological tolerance is established when both signaling to and epigenetic priming of immune response genes are weakened. In parallel, epigenetic priming of immune-repressive genes is boosted, rendering them sensitive to low levels of signaling below the threshold needed to activate immune response genes.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

18 Samples

Download data: TXT

(Submitter supplied) Here we investigated the underlying mechanisms causing the suppression of normal immune responses when T cells are rendered anergic by tolerance induction. By performing an integrated analysis of signaling, epigenetic modifications and gene expression, we demonstrate that immunological tolerance is established when both signaling to and epigenetic priming of immune response genes are weakened. In parallel, epigenetic priming of immune-repressive genes is boosted, rendering them sensitive to low levels of signaling below the threshold needed to activate immune response genes.

Organism:

Mus musculus

Type:

Other

Platform:

GPL19057

14 Samples

Download data: BEDGRAPH