GEO DataSets

(Submitter supplied) 2,3,7,8-TCDD (TCDD) is a reproductive toxicant and endocrine disruptor in nearly all vertebrates, yet the mechanisms by which TCDD induces these reproductive alterations have not been fully characterized. Fish are among the most sensitive vertebrates to the toxic effects of TCDD, and even subtle physiologic changes induced by TCDD can impair reproduction. Previously, we have shown that chronic, sub-lethal exposure to TCDD decreased reproductive capacity, reduced serum estradiol and vitellogenin concentrations, and altered follicular development. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Datasets:

GDS2927 GDS2928

Platform:

GPL1319

8 Samples

Download data: CEL, EXP

Analysis of ovaries following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations of up to 100 parts per billion (ppb). TCDD is a reproductive toxicant and endocrine disruptor in vertebrates. Results provide insight into molecular mechanisms underlying TCDD toxicity.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 4 dose sets

Platform:

GPL1319

Series:

GSE4859

4 Samples

Download data: CEL, EXP

Analysis of ovaries following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations of up to 100 parts per billion (ppb). TCDD is a reproductive toxicant and endocrine disruptor in vertebrates. Results provide insight into molecular mechanisms underlying TCDD toxicity.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 4 dose sets

Platform:

GPL1319

Series:

GSE4859

4 Samples

Download data: CEL, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

81 Samples

Download data: CALLS, PAIR

(Submitter supplied) Juvenile zebrafish were fed Biodiet starter (4% body weight per day) for 42 d with TCDD added at 0 ppb, 0.1 ppb, 1 ppb, 10 ppb or 100 ppb. Fish were collected, sexed, weighed and length measured at 0, 7, 14, 28 or 42 d for TCDD assessment, histopathologic and microarray analysis. Microarray experiments were conducted using 0 and 100 ppb-TCDD treated male and female sexed zebrafish at 7, 14, 28 and 42 d. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

63 Samples

Download data: CALLS, PAIR

(Submitter supplied) Juvenile zebrafish were fed Biodiet starter (4% body weight per day) for 42 d with TCDD added at 0 ppb, 0.1 ppb, 1 ppb, 10 ppb or 100 ppb. Fish were collected, sexed, weighed and length measured at 0, 7, 14, 28 or 42 d for TCDD assessment, histopathologic and microarray analysis. Microarray experiments were conducted in TCDD-treated (at 0, 0.1, 1, 10 and 100 ppb) for male and female sexed zebrafish at 28 d. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL17210

36 Samples

Download data: CALLS, PAIR

(Submitter supplied) In many mammals, halogenated aromatic hydrocarbon (HAH) exposure causes wasting syndrome, defined as lethal weight loss as a result of severe and persistent hypophagia. The most potent HAH in causing wasting is 2,3,7,8-tetrachlorodibenzo-ρ-dioxin (TCDD), which exerts its toxic effects through the aryl hydrocarbon receptor (AHR) – a transcription factor. Because TCDD toxicity is thought to predominantly arise from dysregulation of AHR-transcribed genes, we hypothesized that wasting syndrome is due to TCDD-induced dysregulation of genes involved in regulation of food-intake. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL19144

16 Samples

Download data: CEL, TXT

(Submitter supplied) Rodents exposed to the environmental contaminant, TCDD, suffer from a number of acute and chronic toxicities, including lethality and a wasting syndrome. Hypothesizing that the wasting syndrome may be caused by changes in adipose tissue -- either in its hormonal regulation or in homeostatic effects -- we profiled the transcriptional response of rat white adipose to TCDD. We employed two separate rat strains: the Long-Evans strain is sensitive to TCDD toxicities while the Han/Wistar strain is over four orders of magnitude more resistant. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL9199

28 Samples

Download data: CEL, DAT

(Submitter supplied) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes the many forms of reproductive toxicity, such as defects in sexual behaviors, in pups of which mother is exposed to this substance at lower doses. However, the mechanism underlying these defects remains to be clarified in spite of many researches conducted so far. Our previous studies have revealed that maternal treatment with TCDD attenuates the production of pituitary gonadotropins [luteinizing hormone (LH) and follicle-stimulating hormone] in the late fetuses, leading to the impairment of sexual behavior in adulthood. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6101

12 Samples

Download data: TXT

(Submitter supplied) To fully understand molecular toxicity of TCDD in an in vivo animal model, adult zebrafish were exposed to TCDD at 10 nM for 96 h and the livers were sampled for RNA-sequencing based transcriptomic profiling. A total of 1,058 differently expressed genes were identified based on fold-change>2 and TPM (transcripts per million) >10. Among the top 20 up-regulated genes, 10 novel responsive genes were identified and verified by qRT-PCR analysis on independent samples. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10658

2 Samples

Download data: TXT

(Submitter supplied) This experiment investigates the role of a novel AHR-regulated gene on the global transcriptome in 48 hpf zebrafish exposed to 0.1% DMSO or 1 ng/mL TCDD.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14875

16 Samples

Download data: TXT, XLSX

(Submitter supplied) Pregnant C57Bl6N mice were treated with 0 (corn oil), 1.5, 3.0, or 6.0 ug/kg TCDD on gd14.5. Fetal hearts were collected on gd17.5. Hearts from each litter were pooled onto one chip. 4 replicates of each condition were run on affymetrix MG_U74Av2 chips, using standard affymetrix protocols and controls. Keywords: dose-response, 3 doses plus corn oil control, 4 replicates

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1302

Platform:

GPL81

20 Samples

Download data: CEL

Analysis of gestational day (GD) 17.5 fetal hearts isolated from pregnant C57BL/6Ns treated at GD 14.5 with up to 6.0 ug/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). TCDD, a cardiovascular teratogen, inhibits cardiomyocyte proliferation, a process that peaks between GD 14.5 to 17.5.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 dose sets

Platform:

GPL81

Series:

GSE2812

20 Samples

Download data: CEL