GEO DataSets

(Submitter supplied) Targeted deletion of skNAC in mice resulted in early embryonic lethality with cardiac defects. In order to investigate the molecular mechanism of the cardiac defect, we designed the microarray comparing gene expression of the mutant E11.5 heart to wild type E11.5 heart. Keywords: genetic modification in mouse

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Transcriptome analysis by RNA-seq of tibialis anterior muscle from control and Smyd1 myocyte-specific conditional knockout mice at 6 weeks of age. Smyd1 is a methyltransferase specifically expressed in striated muscle and CD8+ T cells. Smyd1 deficiency resulted in centronuclear myopathy primarily affecting fast-twitch muscle fibers. These results provide insight into how loss of Smyd1 altered transcriptional programs resulting in centronuclear myopathy.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) Myoblasts harvested from a postnatal day 2 WT and Foxj3 KO litter. We used Affymetrix microarrays to identify dysregulated transcripts in Foxj3 mutant myoblasts. Keywords: mutant analysis, skeletal myoblasts

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL, EXP

(Submitter supplied) Heart development is modulated by FOG-2/NuRD interactions. FOG-2R3K5A is unable to recuit the NuRD complex and this results in cardiac defects such as ASD, VSD, and thin ventricular walls We used microarrays to detail the changes in gene expression in FOG-2R3K5A hearts to determine misexpression of genes that may be causing the observed phenotypes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) The regulation of multipotent cardiac progenitor cell (CPC) expansion and subsequent differentiation into cardiomyocytes, smooth muscle, or endothelial cells is a fundamental aspect of basic cardiovascular biology and cardiac regenerative medicine. However, the mechanisms governing these decisions remain unclear. Here, we show that Wnt/β-Catenin signaling, which promotes expansion of CPCs, is negatively regulated by Notch1-mediated control of phosphorylated β-Catenin accumulation within CPCs, and that Notch1 activity in CPCs is required for their differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3811

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of β-Catenin-stabilized cardiac progenitor cells (CPCs) from E9.0 mutant embryos, before cardiac dysfunction. β-Catenin signaling promotes expansion of multipotent CPCs in vivo. Results provide insight into the molecular mechanisms underlying CPC expansion.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE15232

6 Samples

Download data: CEL

(Submitter supplied) Human pluripotent stem cell- (hPSC)-derived skeletal muscle progenitors (SMP)—defined as PAX7-expressing cells with myogenic potential—can provide an abundant source of donor material for muscle stem cell therapy owing to the near-infinite replication potential of PSCs. As in vitro myogenesis is decoupled from in vivo timing and the 3D-embryo structure, it remains difficult to definitively characterize what stage or type of muscle is modeled in culture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

24 Samples

Download data: TXT

(Submitter supplied) The transcription factor NF-Y promotes cell proliferation and often loses its activity during differentiation through the regulation of NF-YA, the DNA binding subunit of the complex. In stem cell compartments, the shorter NF-YA splice variant (NF-YAs) is abundantly expressed and sustains their expansion. Here, we report that satellite cells, the stem cell population of adult skeletal muscle necessary for its growth and regeneration, express uniquely the longer NF-YA isoform (NF-YAl), majorly associated with cell differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

6 Samples

Download data: TXT

(Submitter supplied) Selective suppression of cardiac gene expression by RE-1 silencing transcription factor (REST) in embryonic stage is essential for cardiogenesis and function; however, the underlying mechanism remains unclear. In this study, we show that REST  suppression of cardiac genes during development is temporarily regulated through non-CpG methylation at REST binding sites. Gene expression analyses revealed that the expression of Hcn2 (hyperpolarization-activated cyclic nucleotide-gated ion channel 2), a REST-targeted gene, was developmentally upregulated, while DNMT3B level and REST expression was downregulated. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) The search for developmental mechanisms driving vertebrate organogenesis has paved the way toward a deeper understanding of birth defects. During embryogenesis, parts of the heart and craniofacial muscles arise from pharyngeal mesoderm (PM) progenitors. Here, we reveal a hierarchical regulatory network of a set of transcription factors expressed in the PM that initiates heart and craniofacial organogenesis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4326

Platform:

GPL8321

12 Samples

Download data: CEL

Analysis of pharyngeal mesoderm (PM) myogenic progenitors and trunk myogenic progenitors from E9.5 - E11.5 embryos. During embryogenesis, certain heart and craniofacial muscles arise from PM progenitors. Results provide insight into role of PM regulatory network in myogenesis and cardiogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 3 development stage sets

Platform:

GPL8321

Series:

GSE42389

12 Samples

Download data: CEL

(Submitter supplied) During mammalian organogenesis, the cells of the three germ layers transform into an embryo that includes most major internal and external organs. The key regulators of developmental defects can be studied during this crucial period, but conventional approaches lack the throughput and resolution to obtain a global view of the molecular states and trajectories of a rapidly diversifying and expanding number of cell types. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

760 Samples

Download data: CSV, TXT

(Submitter supplied) Reciprocal interactions between non-myocytes and cardiomyocytes are implicated in the control of cardiac growth and differentiation. Here, we report the identification of early B-cell factor 1 (Ebf1) as a transcription factor highly enriched in non-myocytes that potently regulates heart development. Postnatal Ebf1 deficient hearts are characterized by marked myocardial hypercellularity and reduced cardiomyocyte size, as well as ventricular conduction system hypoplasia and conduction system disease. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

22 Samples

Download data: BW

(Submitter supplied) The main function of chromatin-associated long non coding RNAs (lncRNA) is to tether specific combinations of regulatory factors to target genomic sites. Here we show the characterization of a conserved lncRNA, Charme, that acts as an architectural scaffold to bring together distantly located genomic loci. The functional knock-down of Charme revealed a direct link between the formation of chromosomal loops and the down-regulation of genes therein contained, which are crucial for the correct control of muscle homeostasis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL17021

10 Samples

Download data: BED, TXT

(Submitter supplied) The function and phenotype transitions of heart may be specific to one or other chamber. To provide the omics data sets from chambers separately, we conducted spatial transcriptomics of E10.5 hearts to identify unique gene profiles that correspond to distinct anatomical regions in early cardiac development. We obtained curated expression data consisting of 6,798 genes across 332 individual capture spots. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

1 Sample

Download data: TAR

(Submitter supplied) Cardiogenesis is a tightly-regulated dynamic process through a continuum of differentiation and proliferation events. Transcriptional activation is the predominant early step for initiating cardiac formation in mammals. We then applied ChIP-seq analysis against histone modifications H3K4me1, H3K4me3, H3K27ac and RNAPol II in E10.5 mouse hearts to evaluate the instant activity of transcription factors, which is believed to serve as a powerful tool to study regulatory processes.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: BW

(Submitter supplied) Cardiogenesis is a tightly-regulated dynamic process through a continuum of differentiation and proliferation events. Key factors and pathways governing this process remains incompletely understood. Thus, we conducted bulk RNA-seq of mice hearts from embryonic day 10.5 to postnatal week 8 to investigate the gene expression changes.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

30 Samples

Download data: TXT

(Submitter supplied) During the first weeks after birth, cardiomyocytes within the mouse heart progressively exit the cell cycle, binucleate, and lose regenerative capacity. We have determined that combined pharmacological inhibition of thyroid hormone and adrenergic signaling during postnatal development robustly enhances cardiomyocyte proliferation, retention of diploid cardiomyocytes, and functional cardiac regeneration at postnatal day 14. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

27 Samples

Download data: XLSX