GEO DataSets

(Submitter supplied) Although major differences in the response of tissues were documented in the literature, the molecular mechanisms underlying tissue radiosensitivity are still fragmentary and need to be study more thoroughly. To this end, a genome wide analysis strategy was used to investigate changes in gene expression in the liver following exposition to ionizing radiation. This follows the tissue survey that was previously initiated with other organs and tends to obtain a comprehensive picture of the molecular events underlying tissue radio sensitivity. more...

Organism:

Mus musculus; Rattus norvegicus

Type:

Expression profiling by array

Platforms:

GPL4376 GPL4377

16 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2926

Platform:

GPL887

77 Samples

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(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of Mk differentiation human CD34-positive hematopoietic stem and progenitor cells cultured with thrombopoietin, interleukin-3, and Flt3-ligand. The goal this study was to identify genes involved in the various facets of Mk differentiation including commitment, polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

36 Samples

Download data

(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of phorbol-ester-induced Mk differentiation of the megakaryoblastic CHRF-288-11 (CHRF) cell line – a model system for investigating megakaryopoiesis. The goals of this study were to (1) verify the megakaryocytic nature of the CHRF cell line at the transcriptional level, and (2) extract novel insights into the key facets of Mk maturation including polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

41 Samples

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Temporal analysis of phorbol ester-treated CHRF-288-11 megakaryoblastic cells induced to undergo megakaryocytic (Mk) differentiation and primary Mk (PriMk) cells derived from cytokine-treated CD34+ peripheral blood cells. Results provide insight into molecular mechanisms underlying megakaryopoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, log e ratio, 4 agent, 2 cell line, 13 time sets

Platform:

GPL887

Series:

GSE8914

77 Samples

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(Submitter supplied) To understand the host transcriptional response to S. enterica serovar Choleraesuis (S. Choleraesuis), the first generation Affymetrix porcine GeneChip® was used to identify differentially expressed genes in the mesenteric lymph nodes responding to infection at acute (8 hours (h), 24h, 48h post-inoculation (pi)) and chronic stages (21 days (d) pi) The objectives of this study were to identify and examine the stereotypical gene expression response within the host mesenteric lymph nodes to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) heart from rats exposed to cardiopulmonary bypass (CPB) vs sham-operated controls Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platforms:

GPL154 GPL153

12 Samples

Download data

(Submitter supplied) hearts from rats exposed to cardiopulmonary bypass (CPB) vs sham-operated controls Keywords = rat Keywords = heart Keywords = cytokine Keywords = inflammation Keywords: repeat sample

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL153

6 Samples

Download data

(Submitter supplied) heart from rats exposed to cardiopulmonary bypass (CPB) vs sham-operated controls Keywords = rat Keywords = cardiac Keywords = cytokine Keywords = inflammation Keywords: repeat sample

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL154

6 Samples

Download data

(Submitter supplied) One µg of total RNA from either an individual rat or from a pooled sample was amplified and labeled with a fluorescent dye (either Cy3 or Cy5) using the Low RNA Input Linear Amplification Labeling kit (Agilent Technologies, Palo Alto, CA) following the manufacturer’s protocol. The amount and quality of the resulting fluorescently labeled cRNA was assessed using a Nanodrop ND-100 spectrophometer and an Agilent Bioanalyzer. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL890

20 Samples

Download data

(Submitter supplied) PURPOSE: Hyperoxia is toxic to photoreceptors, and this toxicity may be important in the progress of retinal dystrophies. This microarray study examines gene expression induced in the C57BL/6J mouse retina by hyperoxia over the 14-day period during which photoreceptors first resist, then succumb to, hyperoxia. METHODS: Young adult C57BL/6J mice were exposed to hyperoxia (75% oxygen) for up to 14 days. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL, CHP

(Submitter supplied) Androgens have been postulated to be important modulators of adipose tissue metabolism and fat cell function. In the present study, we investigated the response of male and female mice retroperitoneal adipose tissue to the non-aromatizable androgen dihydrotestosterone (DHT). Adipose tissue samples were obtained in gonadectomized (GDX) animals treated with vehicle (control group), or injected with 0.1mg DHT at 1, 3, 6, 12, 18 and 24h prior to necropsy. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3488

Platform:

GPL81

16 Samples

Download data: CEL

Analysis of retroperitoneal adipose tissue samples from gonadectomized males and females following treatment with dihydrotestosterone (DHT) for up to 24 hours. Results provide insight into the role of DHT in the adipogenesis that occurs in retroperitoneal adipose tissues.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 gender, 7 time sets

Platform:

GPL81

Series:

GSE9631

16 Samples

Download data: CEL

(Submitter supplied) Prostaglandin F2 alpha (PGF2alpha) brings about regression of the bovine corpus luteum (CL). This luteolytic property of PGF2alpha is used in the beef and dairy cattle to synchronize estrus. A limitation of this protocol is an insensitivity of the early CL to luteolytic actions of PGF2alpha. The mechanisms underlying luteal sensitivity (LS) are poorly understood. The early CL has maximum number of PGF2alpha receptors; therefore differences in signaling events might be responsible for LS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3810

1 Sample

Download data: GPR

(Submitter supplied) We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell growth arrest and differentiation in astrocyte RCG-12 cells harboring temperature-sensitive simian virus 40 large T-antigen by using an Affymetrix GeneChip system. Astrocyte RCG-12 cells used in this study were derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2725

Platform:

GPL341

4 Samples

Download data: CEL

Analysis of conditionally immortalized astrocyte RCG-12 cells induced to undergo growth arrest and differentiation by heat inactivation of a temperature-sensitive large T-antigen. Results provide insight into the molecular mechanisms underlying cell growth arrest and differentiation.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL341

Series:

GSE7501

4 Samples

Download data: CEL

(Submitter supplied) Expression profiles in mouse liver exposed to long-term gamma-irradiation were examined to assess in vivo effects of low dose-rate radiation. Three groups of male C57BL/6J mice were exposed to whole body irradiation at dose-rates of 17-20 mGy/day, 0.86-1.0 mGy/day or 0.042-0.050 mGy/day for 401-485 days (cumulative doses were approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively). Expression profiles were produced for RNA isolated from irradiated individual animals and for pooled RNA from sham-irradiated 3 animals for control. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL, CHP

(Submitter supplied) This study aimed to identify genes that are linked with optineurin expression using a combined siRNA-microarray approach Keywords: siRNA treatment study

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2892

Platform:

GPL570

6 Samples

Download data: CEL

Analysis of HeLa cells depleted for optineurin using RNAi knockdown. Mutations in the gene for optineurin are associated with open-angle glaucoma. Results provide insight into the function of optineurin and its role in the pathogenesis of open-angle glaucoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL570

Series:

GSE6819

6 Samples

Download data: CEL