GEO DataSets

(Submitter supplied) Chicken 60-mer oligonucleotide microarray, including 39854 cDNA and ESTs, entire Marek’s disease virus and avian influenza virus genomes, and 150 chicken microRNAs, was developed. Cecal tonsil, ileum, liver and spleen from 6 chickens were selected for hybridization to validate the microarray performance. There are 2886, 2886, 2660, 358, 3208 3355, and 3710 genes significantly expressed between liver and spleen, spleen and cecal tonsil, cecal tonsil and ileum, liver and cecal tonsil, liver and ileum, spleen and ileum at the P<10-7. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL4993

24 Samples

Download data: GPR

(Submitter supplied) Lavender oil (LO) – a commonly used oil in aromatherapy with well defined volatile components linalool and linalyl acetate – use in non-traditional medicine is increasing globally. To understand and provide evidence for the potential positive effects of LO on the body, we have established an animal model for investigating in this current study, orally administered LO effects genome-wide in rat intestine, spleen and liver. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL4135

8 Samples

Download data: TXT

(Submitter supplied) This is a chicken multi-tissue cDNA microarray with 12752 elements. It comprises clones from chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. Sequence information is available from GenBank for all features on the array. Protocol: The clone-set was amplified in 96 well plates and re-arrayed into a 384-well Montage (Millipore) filtration plate to isolate the amplified DNA products. more...

Organism:

Gallus gallus

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(Submitter supplied) Background The application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes. Results We have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non-redundant ESTs were selected and added to an existing collection of clones (4,162) from immune tissues and a chicken bursal cell line (DT40). more...

Organism:

Gallus gallus

5 Series

60 Samples

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(Submitter supplied) The aim of this series of experiments was to gain experience in sepsis gene expression profiling in a well-accepted model of murine polymicrobial abdominal sepsis and begin characterizing (in the parlance of genomics) the sepsis "transcriptome." DESIGN: Prospective animal study. SETTING: University-based animal research facility.SUBJECTS C57BL/6 mice. INTERVENTIONS: After induction of general anesthesia, cecal ligation and puncture were performed to induce peritonitis and polymicrobial sepsis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS164

Platform:

GPL207

6 Samples

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C57/B6 mice subjected either to sham laparotomy or cecal ligation and puncture (CLP) and spleen samples taken 24 hours post surgery. Effort to increase understanding of shock and the adaptive response to injury at the level of the entire genome.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 shock sets

Platform:

GPL207

Series:

GSE510

6 Samples

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(Submitter supplied) For decades, formalin-fixing and paraffin embedding (FFPE) has been routinely used to preserve tissue samples for histological analysis. Global gene expression analysis of these archival tissues has the potential to greatly advance research attempting to link perturbations in molecular pathways to disease outcome. We investigated 16-year-old FFPE mouse liver samples treated with phenobarbital and created a protocol for their analysis using Agilent gene expression arrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

28 Samples

Download data: TXT

(Submitter supplied) Excessive accumulation of lipids in the adipose tissue is a major problem in the present-day broiler industry. However, few studies have analyzed the expression of adipose tissue genes that are involved in pathways and mechanisms leading to adiposity in chickens. Gene expression profiling of chicken adipose tissue could provide key information about the ontogenesis of fatness and clarify the molecular mechanisms underlying obesity. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

10 Samples

Download data: CEL, EXP

(Submitter supplied) Transcriptional profiling of two A. nidulans strains, aiming to compare the control strain CV125 (pabaA1) with the transgenic strain NA1363 (pabaA1) harbouring a gpdAp-lacZ-trpCt.riboB construct integrated in chromosome VIII. This construct, carried out in a derivative of the pBR322 vector, contained the E. coli lacZ sequence (encoding ß-galactosidase) fused to the A. nidulans trpC terminator and placed under the control of the strong and constitutive promoter of the A. more...

Organism:

Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL21331

16 Samples

Download data: TXT

(Submitter supplied) We performed deep sequencing of the miRNAs present in ~20 toxicologically relevant tissues in Sprague Dawley rats and used three independent analysis of the data to determine which miRNAs are tissue specific and tissue enriched.

Organism:

Rattus norvegicus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14844

458 Samples

Download data: TXT

(Submitter supplied) To analyze the effect of different aliments, male OF1 mice were fed ad libitum with a custom-made diet containing 33% maize derived from three cultivars containing comparable nutritional values for multiple generations. To evaluate possible effects on the gene expression in the small intestine, the jejunum of nine randomly chosen males of the F3 was sampled at the age of 7 weeks. Note that the lack of information on the time of sampling or its randomization between the experimental groups limited the value of the internal microarray data set for the context originally addressed (diet).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

9 Samples

Download data: TXT

(Submitter supplied) Deep sequencing as a high-throughput technology has been widely used in the transcriptome profiling in mammals. In the present study, we aimed to identify chicken lncRNAs ranging from 300-1600 nt long. Total RNAs from chicken skeletal muscle at the embryonic stage were fractionated by 6% urea PAGE. Selected RNA fractions (300-1600 nt) were sequenced by Solexa technology.

Organism:

Gallus gallus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9385

1 Sample

Download data: BED, BEDGRAPH

(Submitter supplied) Lung, liver, muscle (Semitendinosus), small intestine (duodenum; approximately 5 cm from stomach) were collected from six cross-bred white pigs (three 15 week old and three 17 week old). Total RNAs were isolated from these tissues to study gene expression by using the first generation of porcine long oligonucleotide microarray. In the experimental design, tissue samples from the six animals were assigned to six loops (three for younger animals and three for older animals). more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL1881

24 Samples

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(Submitter supplied) Zhao SH, Recknor J, Lunney JK, Nettleton D, Kuhar D, Orley S, Tuggle CK: Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig.Zhao SH, Recknor J, Lunney JK, Nettleton D, Kuhar D, Orley S, Tuggle CK., Genomics. 2005 Nov;86(5):618-25. Protocol: Oligonucleotides were printed in duplicate on epoxide slides by operon. Operon probes are designed from The TIGR Gene Index Database SsGI Release 5.0 released 10/1/02. more...

Organism:

Sus scrofa

1 Series

18 Samples

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(Submitter supplied) We compared repeatability and comparability of microRNA microarray using 5 different platforms. and compared microarray data generated from five different microRNA microarray platforms with quantitative RT-PCR. Our data suggested that the most accurate and repeatable methods for microRNA expression profiling are Agilent and Toray, and the numbers of detected microRNA at Toray are more than at Agilent. more...

Organism:

Mus musculus; Rattus norvegicus; Homo sapiens

Type:

Non-coding RNA profiling by array

5 related Platforms

30 Samples

Download data: GPR, TXT

(Submitter supplied) Gene expression profiling of very few or even single cells is of particular interest in many applications. However, detection of a large number of mRNA sequences from a small number of cells is limited by the sensitivity of available methods. High-throughput multiplex reverse transcription followed by PCR amplification (RT-PCR) has much to offer to these studies due to its inherent sensitivity, efficiency and cost-effectiveness. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4370

4 Samples

Download data: GPR

(Submitter supplied) We describe the design, cleaning and annotation of the gene index for sunflower and the construction of the microarray platform. In addition, we validate this tool by means of the analysis of global changes in gene expression profiles in response to water deficit as a physiological event which induces senescence, taken as a model experiment, for which reference genes have also been identified and validated (Fernandez et al., 2011). more...

Organism:

Helianthus annuus

Type:

Expression profiling by array

Platform:

GPL13610

8 Samples

Download data: TXT

(Submitter supplied) The mechanisms responsible for the molecular pathogenesis of the highly pathogenic avian influenza virus (HPAIV) or low pathogenic avian influenza virus (LPAIV) in avian species remain poorly understood. Thus, global immune response of chickens infected with HPAIV H5N1 (A/duck/India/02CA10/2011) and LPAIV H9N2 (A/duck/India/249800/2010) viruses was studied using microarray to identify crucial host genetic components responsive to these infection. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL19667

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus; Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by array

4 related Platforms

228 Samples

Download data: CEL, CHP

(Submitter supplied) We used the microarray data to analyze host cells response on A549 cells infected with Influenza A virus (A/Singapore/478/2009 (pH1N1))

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

18 Samples

Download data: CEL, CHP