GEO DataSets

(Submitter supplied) Brucella dynamically engage macrophages while trafficking to an intracellular replicative niche as macrophages, the first line of innate host defense, attempt to eliminate organisms. Brucella melitensis, B. neotomae, and B. ovis are highly homologous, yet exhibit a range of host pathogenicity and specificity. RAW 264.7 macrophages infected with B. melitensis, and B. ovis exhibit divergent patterns of bacterial persistence and clearance; conversely, B. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

11 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platforms:

GPL81 GPL32

16 Samples

Download data: CEL, CHP

(Submitter supplied) Facultative intracellular Brucella infect and survive inside macrophages, and the outcome of macrophage-Brucella interaction is a basis for establishment of a chronic Brucella infection. The majority of Brucella are killed at the early infection stage. A subpopulation of virulent Brucella strains is instead trafficked to an intracellular replicative phagosome, and are resistant to further attack and begin to multiply dramatically. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella ovis; Brucella canis; Brucella melitensis

Type:

Expression profiling by array

Platforms:

GPL15024 GPL15023

16 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella ovis; Brucella canis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella canis; Brucella ovis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella ovis; Brucella canis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL15023

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) The virB operon, encoding a Type IV secretion system (T4SS), is essential for intracellular survival and persistent infection of Brucella spp. To better understand the role of the T4SS in evading host defense mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild type Brucella strains and strains that fail to express the virB genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2859

Platform:

GPL1261

10 Samples

Download data: CEL

Analysis of splenocytes from animals infected with virB mutant Brucella arbortus strains. The virB operon encodes a Type IV secretion system (T4SS) essential for the intracellular survival and persistent infection by Brucella species. Results provide insight into the role of T4SS in infections.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 genotype/variation, 3 infection sets

Platform:

GPL1261

Series:

GSE6810

10 Samples

Download data: CEL

(Submitter supplied) Identification of host responses at the gene transcription level provides a molecular profile of the events that occur following infection. Brucella abortus is a facultative intracellular pathogen of macrophages that induces chronic infection in humans and domestic animals. Using microarray technology, the response of macrophages 4 hours following B. abortus infection was analyzed to identify early intracellular infection events that occur in macrophages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL32

5 Samples

Download data: CEL

(Submitter supplied) In a time course study, we characterized global gene expression profile of B. melitensis-infected bovine Peyer patches in the first 4 h p.i. Microarray analysis revealed that 2,916 bovine genes were detected as differentially expressed (z-score p < 0.025) in loops inoculated with virulent B. melitensis 16M compared with controls between 15 min and 4 h post-infection. From these genes, 2,286 (78%) were up- and 630 (22%) were down-regulated. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

20 Samples

Download data: GPR

(Submitter supplied) The macrophage-Brucella interaction is critical for the establishment of a chronic Brucella infection. Smooth virulent B. suis strain 1330 (S1330) prevents macrophage cell death. However, rough attenuated B. suis strain VTRS1 induces strong macrophage cell death. To further investigate the mechanism of VTRS1-induced macrophage cell death, microarrays were used to analyze temporal transcriptional responses of murine macrophage-like J774. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4432

Platform:

GPL1261

42 Samples

Download data: CEL

Analysis of J774.A1 macrophage cells for up to 48 hours following infection with S1330-smooth virulent or VTRS1-rough attenuated Brucella suis strain. S1330 prevents macrophage cell death while VTRS1 induces it. Results provide insight into the molecular basis of VTRS1-induced macrophage cell death.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 infection, 7 time sets

Platform:

GPL1261

Series:

GSE21117

42 Samples

Download data: CEL

(Submitter supplied) Hfq is a bacterial Sm-like protein which serves as a post-transcriptional regulator of global gene expression. It has been shown to be involved in a wide range of cellular processes and pathways. To characterize the regulation roles of Hfq in Brucella melitensis, the in vitro hfq induction conditions simulating intracellular environments were firstly defined, and then, the transcriptome of the hfq mutant was compared to that of the wild type strain. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) Brucella ovis causes an important disease characterized by decreased fertility in rams, sporadic abortions in ewes and increased lamb mortality. The live attenuated B. melitensis Rev.1 vaccine is considered the best vaccine available against this infection. However, this vaccine shows variable protective efficacy ranging from 40% to 100%.The objective of this study was to identify possible correlates of protective response to B. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL6954

9 Samples

Download data

(Submitter supplied) A mini-microarray containing spotted oligos corresponding to 289 B. melitensis genes was used to identify candidate genes whose transcription is influenced by BlxR. This microarray contains genes encoding the type IV secretion system, flagella, transcriptional regulators, transporters, and proteins involved in outer membrane biogenesis, iron acquisition, and denitrification. RNA was prepared from both B. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6170

14 Samples

Download data

(Submitter supplied) Gene expression analysis of wild-type and STING knock-out mouse bone marrow-derived macrophages (mBMDM) infected with Brucella abortus or transfected with Brucella abortus DNA. Genes whose expression are affected by Brucella abortus in a STING-dependent manner will be identified and signaling pathways regulated by STING will be elucidated.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL, CHP

(Submitter supplied) Infection of sheep with Brucella ovis results in ovine brucellosis, a disease characterized by infertility in rams, abortion in ewes and increased perinatal mortality in lambs. During the course of the infection both the ovine immune response and host cell gene expression are modified. The objective of this research was to conduct a preliminary characterization of differential gene expression in rams experimentally infected with B. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL6954

6 Samples

Download data

(Submitter supplied) In this study we report that B. melitensis at the late logarithmic phase of growth are more invasive for HeLa cells than at mid logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase compared to the stationary growth phase. The vast majority of the up-regulated genes in late-log cultures were those associated with DNA replication, transcription and translation, intermediate metabolism, energy production and conversion, membrane transport and cell envelope, biogenesis and outer membrane, while the down-regulated genes were distributed among several functional categories. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6748

8 Samples

Download data: GPR