GEO DataSets

(Submitter supplied) In this study, using DNA microarray analysis, we compared the comprehensive expression profiles of two yeast trains, i.e, the previously obtained ethanol-adapted yeast strain and the parental strain as control (FY834), under the ethanol stress condition (YPD medium contained 10% ethanol). As a result, we identified certain genes and functional categories of the genes that are possible involved in growth under the ethanol stress condition.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL7698

2 Samples

Download data: GPR

(Submitter supplied) Transcriptional regulation of branched-chain amino acid metabolism in Saccharomyces cerevisiae involves two key regulator proteins, Leu3p and Gcn4p. Leu3p is a pathway-specific regulator, known to regulate six genes involved in branched-chain amino acid metabolism and one gene in nitrogen assimilation. Gcn4p is a global regulator, involved in the general response to amino acid and purine starvation. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1103

Platform:

GPL90

12 Samples

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Analysis of leu3 mutant grown in either limited ethanol or limited ammonium media. Leu3p regulates a gene involved in nitrogen assimilation and six genes involved in branched chain amino acid metabolism. Results provide insight into the role of Leu3p in gene regulation.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 genotype/variation, 2 growth protocol sets

Platform:

GPL90

Series:

GSE2076

12 Samples

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(Submitter supplied) This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool, and the BY4743 parental strain were grown for 18 hours in a rotating wall vessel, a suspension culture device optimized to minimize the delivered shear. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL90 GPL2544

12 Samples

Download data

(Submitter supplied) Rsf1p is a putative transcription factor required for efficient growth using glycerol as sole carbon source but not for growth on the alternative respiratory carbon source ethanol. We use microarrays to determine the differences in the transcriptional program between the Δrsf1 mutant and the wild type during respiratory growth on glycerol as well as the transition to growth on glycerol as sole carbon source. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

22 Samples

Download data: CEL, CHP

(Submitter supplied) The vanillin tolerance Saccharomyces cerevisiae was screened and compared intracellular ergosterol levels with several laboratory yeast strains, to study potential relationship between ergosterol contents and vanillin tolerance. S. cerevisiae NBRC1950 was selected as a vanillin tolerant strain. Its ergosterol contents were higher than those of laboratory strains. The results of DNA microarray and quantitative RT-PCR analysis showed that 5 genes involved in ergosterol biosynthesis (ERG28, HMG1, MCR1, ERG5 and ERG7) were up-regulated in NBRC 1950 compared with strain X2180, suggested that high expressions of genes involved in ergosterol biosynthesis may cause for the high ergosterol content in strain NBRC 1950. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

4 Samples

Download data: CEL, CHP

(Submitter supplied) affymetrix gene expression data over three successive metabolic cycles Keywords: yeast, time course, metabolic cycle

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2267

Platform:

GPL90

36 Samples

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Analysis of nutrient-limited continuous-culture cells at 12 25-minute intervals for 3 cycles. Cells grown under such conditions exhibit robust, periodic cycles in the form of respiratory bursts. Results provide insight into molecular mechanisms responsible for controlling metabolic oscillations.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 3 protocol, 12 time sets

Platform:

GPL90

Series:

GSE3431

36 Samples

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(Submitter supplied) We have previously shown that fed-batch processes with the longest uncoupling phase (ethanol adapted) were characterized by induction of storage carbohydrates, a metabolic event typical of yeast cells experiencing nutrient limitation, at the onset of this phase, whereas this metabolic event was not seen in processes with a short uncoupling phase (ethanol non adapted culture). Taken together, our results suggested that reproducible high bioethanol performance in aerated fed-batch process may be linked to the ability of yeast cells to impede ethanol toxicity by triggering a metabolic remodelling reminiscent to that of cells entering a quiescent G0/G1 state. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL8327

4 Samples

Download data: GPR

(Submitter supplied) Yeast S. cerevisiae microarrays from the SGDB (Ecole Normale Superieure, Paris, France). Yeast ORFs are deposited in duplicate (double genome). Probes are oligonucleotides (40mers) from the Yeast oligoset distributed by MWG Biotech. Keywords = yeast Keywords = transcriptome

Organism:

Saccharomyces cerevisiae

2 Series

5 Samples

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(Submitter supplied) The effect of the heavy metal copper on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. Gene expression profile of baker’s yeast Saccharomyces cerevisiae grown in a media containing sub-lethal concentration of cupric sulfate was compared with those of yeast grown in a normal media. Among about 6200 ORFs of yeast, 143 ORFs were induced more than two-fold to resist against copper toxicity after exposure to copper. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1945

5 Samples

Download data: GPR

(Submitter supplied) The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A recombinant S. cerevisiae flocculent strain that efficiently ferments lactose to ethanol was previously obtained by evolutionary engineering of an original recombinant that displayed poor lactose fermentation performance. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL7120

4 Samples

Download data: TXT

(Submitter supplied) To identify genes involved in acclimatization of yeast to non-lethal ethanol stress, S. cerevisiae strain W303-1A was grown separately at 0% and 6.5% (v/v) ethanol, and gene expression profiles were compared using microarray analysis. A t-test with a p-value of 0.05 was applied for differences in gene expression across unstressed and stressed replicates of W303-1A. There were 1049 genes with significant differences in expression. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4873

6 Samples

Download data: GPR

(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Reference: Xue Y, Haas SA, Brino L, Gusnanto A et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 2004 Jan 15;21(1):25-39. PMID: 14745780 Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). more...

Organism:

Schizosaccharomyces pombe

3 Series

3 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. more...

Organism:

Schizosaccharomyces pombe

4 Series

14 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray

Organism:

Schizosaccharomyces pombe

1 Series

2 Samples

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(Submitter supplied) Gene expression profiles of baker’s yeast during initial dough-fermentation were investigated using liquid fermentation media to obtain insights at the molecular level into rapid adaptation mechanisms of baker’s yeast. Results showed that onset of fermentation caused drastic changes in gene expression profiles within 15 min. Genes involved in the tricarboxylic acid (TCA) cycle were down-regulated and genes involved in glycolysis were up-regulated, indicating a metabolic shift from respiration to fermentation. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

6 Samples

Download data

(Submitter supplied) Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed. The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then the Qiagen RNeasy Midi Kit with some modifications within the manufacturers´ protocol and the Qiagen RNase-Free DNase Set were applied. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL7145

10 Samples

Download data: GPR

(Submitter supplied) Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2498

Platform:

GPL2529

12 Samples

Download data: CEL

Analysis of spt15-300 mutant grown in conditions of ethanol and glucose stress. Mutant generated by global transcription machinery engineering (gTME) and bears 3 mutations in SPT15. Results provide insight into the applicability of gTME to alter cellular eukaryotic phenotypes.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 strain, 2 stress sets

Platform:

GPL2529

Series:

GSE5185

12 Samples

Download data: CEL