GEO DataSets

(Submitter supplied) Objectives. Sex-associated differences have been identified in the anatomy, physiology and pathophysiology of the human cornea. We hypothesize that many of these differences are due to fundamental variations in gene expression. Our objective in this study was to determine whether such differences do exist in human corneal epithelial cells both in vivo and in vitro. Methods. Human corneal epithelial cells were isolated from the corneoscleral rims of male and female donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL8159 GPL571 GPL8158

16 Samples

Download data: XLS

(Submitter supplied) Microarray was used to study global gene expression of a cell culture model based on SV40-immortalized human corneal epithelial (iHCE) cells. The gene expression profile of the cell line was compared to the normal human corneal epithelium. Affymetrix HG-U133A GeneChips® were used for microarray experiments and results were validated by performing RT-qPCR for selected genes. iHCE was found to over- and under-express 22 % and 14 % of the annotated genes, respectively. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

3 Samples

Download data: CEL

(Submitter supplied) The specialized corneal epithelium requires differentiated properties, specific for its role at the anterior surface of the eye, thus tight maintenance of the differentiated qualities of the corneal epithelial is essential. Our studies have focused on pinin (PNN), an exon junction component (EJC) that has dramatic implications on corneal epithelial cell differentiation and may act as a stabilizer of the corneal epithelial cell phenotype. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

32 Samples

Download data: XLSX

(Submitter supplied) PURPOSE. To determine global mRNA expression levels in the corneal and conjunctival epithelia and identify transcripts that exhibit preferential tissue expression. METHODS. cDNA samples derived from human conjunctival and corneal epithelia were hybridized in three independent experiments to a commercial oligonucleotide array representing > 22,000 transcripts. The resulting signal intensities and transcript present/absent calls by the primary microarray analyzing software were used in conjunction with a statistical method to identify transcripts that are preferentially or exclusively (transcripts absent in all three replicates in the other tissue) and significantly (expression >1% of the ß-actin level) expressed in one of the two tissues. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2682

Platform:

GPL96

6 Samples

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Analysis of conjunctival (Cnj) and corneal (Co) epithelia, the main cellular components of the ocular surface. Cnj and Co epithelia are embryologically related but phenotypically and functionally disparate. Results provide insight into the molecular basis of these differences.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL96

Series:

GSE5543

6 Samples

Download data

(Submitter supplied) Recently we had discovered a solid cord like structure at the limbus of human eyes, termed as the Limbal Epithelial Crypt (LEC). It arises from the undersurface of the interpalisade rete ridges and extends towards the conjunctiva over the conjunctival stroma. Anatomical and immunohistochemical studies have shown it to be potentially a Stem Cell Niche. To confirm this hypothesis we conducted comparative gene expression profile of LEC with pathway and Geneontology studies in comparison with other ocular surface epithelial regions such as cornea, limbus, LEC stroma and conjunctiva. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9677

18 Samples

Download data: GPR

(Submitter supplied) To understand the gene-regulatory role of H19 in corneal epithelial cells and to explore whether CLIM activation of H19 might account for non-cell proliferation effects of CLIM, we used siRNA to knock down H19 in primary human corneal epithelial cells. Microarray gene expression analysis revealed that 1,249 genes are down regulated 1.5 fold or more by siH19 compared to scramble control, and that 944 genes are up regulated

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

6 Samples

Download data: CEL, TXT

(Submitter supplied) Cofactors of LIM domain proteins (CLIM1 and CLIM2) are widely expressed transcriptional cofactors that are recruited to gene regulatory regions by DNA-binding proteins, including LIM domain transcription factors. In the cornea, epithelial specific expression of a dominant negative (DN) CLIM under the Keratin 14 (K14) promoter causes blistering, inflammation, epithelial hyperplasia and neovascularization, followed by epithelial thinning and subsequent epidermal-like differentiation of the cornea epithelium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) Clim1 and Clim2 are transcriptional cofactors that mediate protein-protein interactions within DNA-binding complexes in many cell types. Epithelial specific expression of a dominant negative Clim construct causes epithelial hyperplasia, neovascularization, inflammation, and blistering, followed by epithelial thinning and subsequent epidermal-like differentiation of the cornea epithelium. This phenotype resembles some aspects of limbal stem cell deficiency, suggesting that Clim proteins may be involved in regulating stem cell mainenance for the cornea. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: BED

(Submitter supplied) Limbal vs. corneal epithelial basal cell gene expression patterns were identified and compared Keywords: repeat

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2433

Platform:

GPL1261

16 Samples

Download data: CEL, EXP

Analysis of microdissected resting limbal epithelial basal cells (LE) and central corneal epithelial (CE) basal cells of the eye. LE are enriched in corneal epithelial stem cells, CE in transient amplifying cells. Results provide insight into the molecular nature of the two basal cell populations.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 8 individual, 2 tissue sets

Platform:

GPL1261

Series:

GSE4098

16 Samples

Download data: CEL, EXP

(Submitter supplied) Series includes pooled (n = 5 mice per biological replicate) samples from submandibular, sublingual, parotid, lacrimal, and meibomian glands of BALB/c mice. Both male and female samples were analyzed on CodeLink Mouse Uniset I Microarrays. Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1009

Platform:

GPL1310

26 Samples

Download data

Expression profiling of 9 week old BALB/c male and female submandibular, sublingual, and parotid salivary glands. Lacrimal and meibomian glands also examined. Results provide insight into the differences between male and female salivary glands.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 gender, 5 tissue sets

Platform:

GPL1310

Series:

GSE1503

26 Samples

Download data

(Submitter supplied) The identification of a marker that is expressed in the conjunctival epithelium but not in the corneal epithelium has been a growing need. A more specific marker of limbal and conjunctival epithelia would be necessary to detect non-corneal epithelial cells on the corneal surface. To search for conjunctival specific marker(s), we first performed preferential gene profiling in the conjunctiva in direct comparison to that in the cornea using microarray technique.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) Pnn depletion in developing mouse corneal epithelium led to disrupted alternative splicing of multiple ESRP-regulated epithelial-type exons. In human corneal epithelial cells (HCET), ESRP1 and PNN displayed close localization in and around nuclear speckles and their physical association in protein complexes was identified. In this study, gene expression profiling was performed to identify PNN- and ESRP1-regulated alternative pre-mRNA splicing in human corneal epithelial cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL16254 GPL16238

48 Samples

Download data: CEL

(Submitter supplied) human corneal epithelial cells were isolated from healthy human donor eyes. Cells were cultured with irradiated 3T3 (i3T3) murine fibroblast feeder cells, irradiated human corneal fibroblasts (iHFL) or without feeder layer

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

8 Samples

Download data: TXT

(Submitter supplied) Corneal injuries remain a major cause of consultation in the ophthalmology clinics worldwide. Repair of corneal wounds is a complex mechanism that involves cell death, migration, proliferation, differentiation, and extracellular matrix (ECM) remodeling. In the present study, we used a tissue-engineered, two-layers (epithelium and stroma) human cornea as a biomaterial to study both the cellular and molecular mechanisms of wound healing. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

27 Samples

Download data: TXT

(Submitter supplied) Immortalized human corneal, conjunctival, and meibomian gland epithelial cells were pre-treated with dihydrotesteosterone (DHT) or vehicle, then exposed to lipopolysaccharide (LPS) and ligand binding protein (LBP) or vehicle, lysed, and subjected to microarray analysis of RNA expression.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

36 Samples

Download data: IDAT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL6480 GPL9081

10 Samples

Download data: TXT

(Submitter supplied) To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation. We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

2 Samples

Download data: TXT