GEO DataSets

(Submitter supplied) Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation. Keywords: Treatment, signaling, one-colour array, signaling pathway comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

15 Samples

Download data: CEL, CHP

(Submitter supplied) Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation. Keywords: Teatment, signaling, one-colour array, signaling pathway comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

15 Samples

Download data: CEL

(Submitter supplied) We set out to generate transcriptional maps of chondrocyte UPR gene networks in vivo using two mouse models (Schmid and Cog) of Schmid chondrodysplasia, in order to define the consequences of UPR activation for the adaptation, differentiation, and survival of chondrocytes experiencing ER stress during hypertrophy, thus providing insights into ER stress signaling and its impact on cartilage pathophysiology. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

12 Samples

Download data: TXT

(Submitter supplied) Background: Glucocorticoids (GCs) are widely used anti-inflammatory drugs. While useful in clinical practice, patients taking GCs often suffer from skeletal side effects including growth retardation and decreased bone quality in adults. On a physiological level, GCs have been implicated in the regulation of chondrogenesis and osteoblast differentiation, as well as maintaining homeostasis in cartilage and bone. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2802

Platform:

GPL1261

12 Samples

Download data: CEL, CHP, EXP

Analysis of primary cultured fetal chondrocytes treated with pharmacological doses of dexamethasone (DEX) for 6 and 24 hours. DEX is a synthetic glucorticoid (GC) receptor agonist. Results provide insight into the effects of pharmacological GC on chondrocytes.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL1261

Series:

GSE7683

12 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Articular and growth plate cartilage have comparable structures consisting of three distinct layers of chondrocytes, suggesting similar differentiation programs and therefore similar gene expression profiles. To address this hypothesis and to explore transcriptional changes that occur during the onset of articular and growth plate cartilage divergence, we used microdissection of 10-day-old rat proximal tibial epiphyses, microarray analysis, and bioinformatics to compare gene expression profiles in individual layers of articular and growth plate cartilage. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS5434

Platform:

GPL6247

12 Samples

Download data: CEL

(Submitter supplied) We used laser capture microdissection to isolate different zones of the articular cartilage from proximal tibiae of 1-week old mice, and used microarray to analyze global gene expression. Bioinformatic analysis corroborated previously known signaling pathways, such as Wnt and Bmp signaling, and implicated novel pathways, such as ephrin and integrin signaling, for spatially associated articular chondrocyte differentiation and proliferation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5433

Platform:

GPL1261

14 Samples

Download data: CEL, CHP

Analysis of superficial (SZ) and intermediate/deep (IDZ) zones microdissected from articular cartilage and resting (RZ) zone from growth plate cartilage of proximal tibial epiphyses of 10-day old rats. Results provide insight into molecular basis of articular and growth plate cartilage divergence.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 3 tissue sets

Platform:

GPL6247

Series:

GSE54216

12 Samples

Download data: CEL

Analysis of superficial (SZ), intermediate/deep (IDZ), and resting (RZ) zones microdissected from articular cartilage of the proximal tibiae of 1-week old mice. Results provide insight into the spatial regulation of gene expression in articular cartilage.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 tissue sets

Platform:

GPL1261

Series:

GSE51994

14 Samples

Download data: CEL, CHP

(Submitter supplied) In the growth plate, the reserve and perichondral zones have been hypothesized to have similar functions, but their exact functions are poorly understood. Our hypothesis was that significant differential gene expression exists between perichondral and reserve chondrocytes that may differentiate the respective functions of these two zones. Normal Sprague-Dawley rat growth plate chondrocytes from the perichondral zone (PC), reserve zone (RZ), proliferative zone (PZ), and hypertrophic zone (HZ) were isolated by laser microdissection and then subjected to microarray analysis. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

8 Samples

Download data: CEL, CHP

(Submitter supplied) A variety of cell cultures models and in vivo approaches have been used to study gene expression during chondrocyte differentiation. The extent to which the in vitro models reflect bona fide gene regulation in the growth plate has not been quantified. In addition, studies that evaluate global gene expression changes among different growth plate zones are limited. To address these issues, we completed a microarray screen of three growth plate zones derived from manually segmented embryonic mouse tibiae. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS3733

Platform:

GPL2112

12 Samples

Download data: CEL

Analysis of growth plate chondrocytes, representing reserve and hypertrophic zones (RZ and HZ, respectively), isolated from metacarpals of prepubertal males. RZ cells terminally differentiate into non-dividing HZ cells. Results provide insight into the molecular basis of chondrocyte development.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 2 cell type sets

Platform:

GPL2112

Series:

GSE18738

12 Samples

Download data: CEL

(Submitter supplied) Chondrocytes at different maturation states in the growth plate produce matrix vesicles (MVs), membrane organelles found in the extracellular matrix, with a wide range of contents, such as matrix processing enzymes and receptors for hormones. We have shown that MVs harvested from growth zone (GC) chondrocyte cultures contain abundant small RNAs, including miRNAs. Here, we determined whether RNA also exists in MVs produced by less mature resting zone (RC) chondrocytes and, if so, whether it differs from the RNA in MVs produced by GC cells. more...

Organism:

Rattus norvegicus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18694

4 Samples

Download data: TAB

(Submitter supplied) To explore the mechanisms responsible for spatial and temporal regulation of the growth plate, we microdissected postnatal rat growth plates into their constituent zones and then used microarray analysis to characterize the changes in gene expression that occur as chondrocytes undergo spatially-associated differentiation and temporally-associated senescence.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

35 Samples

Download data: CEL

(Submitter supplied) Human adult height reflects the outcome of childhood skeletal growth. Growth plate (epiphyseal) chondrocytes are key determinants of height. As epiphyseal chondrocytes mature and proliferate, they pass through three developmental stages, which are organized into three distinct layers in the growth plate: (i) resting (round), (ii) proliferative (flat), and (iii) hypertrophic. Recent genomewide association studies (GWASs) of human height identified numerous associated loci, which are enriched for genes expressed in growth plate chondrocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

4 Samples

Download data: XLSX

(Submitter supplied) To test the consequence of reduced glycolysis and reduced cytoplasimic acetyl-CoA in mouse skeletal devleopment. To reduce glycolysis, Ldha was homozygously deleted in collagen 2 expressing chondrocytes using the Cre-lox sytem [Col2-Cre:Ldha(fl/fl)] on top of one allele germline deletion of Ldhb [Ldhb(+/-)]. To reduce cytoplasmic/nuclear acetyl-CoA, Acly that catalizes conversion of citreate into acetyl-CoA was deleted in chondrocytes using Col2-Cre. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28457

12 Samples

Download data: PDF, TXT

(Submitter supplied) This study was undertaken to uncover the molecular pathways that underlie differences among individuals in rates of longitudinal growth and mature length of the long bones in a rodent model. To do so, the transcriptomic profiles of the proximal epiphyses of 14-day old CD-1 wildtype mice and the Longshanks mice were compared. The Longshanks mouse was selectively bred for longer tibiae in relation to body mass (see Marchini et al 2014 – BMC Evol Biol 14:258). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15907

6 Samples

Download data: TSV

(Submitter supplied) To investigate the role of Mapk7 in vertebral developmemnt, we constructed Col2a1-cre;Mapk7f/f transgenic mouse model to delete Mapk7 in cartilage

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) We investigated the molecular mechanisms that regulate the self-renewal and differentiation of chondroprogenitors by comparing the transcriptome profiles between resting chondrocytes and proliferative chondrocytes

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

8 Samples

Download data: CSV