GEO DataSets

(Submitter supplied) Neurotrophins are growth factors that are known to have a role in promoting cell survival and differentiation. The focus of the current study is to examine the role of neurotrophins in regulating ovarian primordial follicle development. Ovaries from 4-day old rats were placed into organ culture and cultured for 10 days in the absence or presence of neurotrophin-3 (NT3), brain-derived neurotrophic factor (BDNF), or nerve growth factor (NGF). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3624

Platform:

GPL1355

6 Samples

Download data: CEL

Analysis of 4 day old ovaries cultured in the presence of neurotrophin-3 (NT3) for 2 days. Neurotrophins are growth factors involved in promoting cell survival and differentiation. Results provide insight into the role of NT3 in ovarian primordial follicle development.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1355

Series:

GSE20358

6 Samples

Download data: CEL

(Submitter supplied) Female fertility is determined in part by the size and development of the primordial follicle pool. The current study investigates the role of glial cell-line derived neurotrophic factor (GDNF) in the regulation of primordial follicle development in the ovary. Ovaries from four-day old female rat pups were maintained in organ culture for ten days in the absence (control) or presence of GDNF or kit ligand/stem cell factor (KL). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

4 Samples

Download data: CEL

(Submitter supplied) The oocytes found within the primordial follicles of mammalian ovaries remain quiescent for months to years until they receive the appropriate signals to undergo the primordial to primary follicle transition and initiate folliculogenesis. The molecular mechanisms and extracellular signaling factors that regulate this process remain to be fully elucidated. The current study investigates the mechanisms utilized by anti-Müllerian hormone (AMH; i.e. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3199

Platform:

GPL1355

4 Samples

Download data: CEL

Analysis of postnatal day 4 ovaries cultured with anti-Mullerian hormone (AMH) for 10 days. AMH is an extracellular signaling molecule that inhibits the primordial to primary follicle transition in ovary. Results provide insight into molecular mechanisms underlying primordial follicle development.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1355

Series:

GSE10557

4 Samples

Download data: CEL

(Submitter supplied) Primordial follicle assembly is a process that occurs in the embryonic or early post natal ovary in which oocyte nests break down to form individual primordial follicles. The size of this initial pool of primordial follicles in part determines the reproductive lifespan of the female. Connective tissue growth factor (CTGF) was identified as a potential regulatory candidate for this process in a previous microarray analysis of follicle development. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

6 Samples

Download data: CEL

(Submitter supplied) The assembly of the developmentally arrested primordial follicle and subsequent transition to the primary follicle are poorly understood processes critical to ovarian biology. Abnormal primordial follicle development can lead to pathologies such as premature ovarian failure. The current study used a genome-wide expression profile to investigate primordial follicle assembly and development. Rat ovaries with predominantly unassembled, primordial, or primary follicles were obtained. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3006

Platform:

GPL85

6 Samples

Download data: CEL

Analysis of ovaries with unassembled, primordial, and primary follicles. Abnormal primordial follicle development can cause pathologies such as premature ovarian failure. Results provide insight into molecular mechanisms underlying primordial follicle assembly and transition to primary follicles.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 development stage, 3 protocol sets

Platform:

GPL85

Series:

GSE9300

6 Samples

Download data: CEL

(Submitter supplied) The dormant population of ovarian primordial follicles is determined at birth and serves as the reservoir for future female fertility. Of equal importance to fertility is the rate that primordial follicles activate and enter folliculogenesis, yet our understanding of the molecular, biochemical, and cellular processes underpinning primordial follicle activation remains limited. The survival of primordial follicles relies on the correct complement and morphology of granulosa cells, which provide signalling factors essential for oocyte and follicular survival. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

8 Samples

Download data: TXT

(Submitter supplied) Follicle assembly is the process by which groups or ‘nests’ of oocytes break down to form primordial follicles. The size of the primordial follicle pool is the major determinant of the reproductive lifespan of a female. Previously, progesterone (P4) has been shown to inhibit follicle assembly, while tumor necrosis factor alpha (TNF-alpha) has been shown to promote the apoptosis that is necessary for follicle assembly. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2783

Platform:

GPL1355

4 Samples

Download data: CEL

Analysis of cultured neonatal ovaries treated with progesterone for 2 days. In the early postnatal period, groups of oocytes break down to form primordial follicles. This process is called follicle assembly. Results provide insight into the role of progesterone in follicle assembly.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1355

Series:

GSE8238

4 Samples

Download data: CEL

(Submitter supplied) The current study was designed to investigate the actions of Anti-Müllerian Hormone (AMH) on primordial follicle assembly. Ovarian primordial follicles develop from the breakdown of oocyte nests during fetal development for the human and immediately after birth in rodents. AMH was found to inhibit primordial follicle assembly, decrease the initial primordial follicle pool size and promote the persistence of small oocyte nests in a rat ovarian organ culture. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

6 Samples

Download data: CEL

(Submitter supplied) Primordial follicle assembly is the process by which ovarian primordial follicles are formed. During follicle assembly oocyte nests break down and a layer of pre-granulosa cells surrounds individual oocytes to form primordial follicles. The pool of primordial follicles formed is the source of oocytes for ovulation during a female’s reproductive life. Complex networks of cellular signaling and gene expression are essential for any biological process. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

27 Samples

Download data: CEL

(Submitter supplied) The ovarian tissues at E16.5, postnatal 0 day (PD0 ) and 3 days (PD3) in mice were collected to perform single-cell RNA sequence (scRNA-seq) in this study. The transcriptome landscape of different cell types contributed to discern the intrinsic mechanism of early folliculogenesis, and the developmental stage-specific germline and somatic cells were defined to reveal their genetic programs along with follicle development as well as their regulative molecules and interaction signals. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: TXT

(Submitter supplied) The ovarian reserve of follicles comprises all oocytes for lifetime fertility and once formed, it is non renewing. The orphan nuclear receptor steroidogenic factor 1 (SF-1; Nr5a1) is essential for steroidogenesis, but its role in the earliest stages of follicular development is not known. We therefore developed a model of conditional depletion of SF-1 from prenatal ovaries and performed RNAsequencing to identify SF-1 regulated genes.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

24 Samples

Download data: TXT, XLSX

(Submitter supplied) The ovarian reserve of follicles comprises all oocytes for lifetime fertility and is depleted by progressive activation. The orphan nuclear receptor liver receptor homolog 1 (LRH-1; Nr5a2) is essential for ovulation, but its role in the early stages of follicular development is not known. We therefore developed a model of conditional depletion of LRH-1 from early postnatal ovaries (postnatal day 4) and performed RNAsequencing to identify LRH-1 regulated genes during the earliest stages of follicular activation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

8 Samples

Download data: TXT, XLSX

(Submitter supplied) Ovarian primordial follicles are critical for female reproduction and comprise a finite pool of gametes arrested in development. A systems biology approach was used to identify regulatory gene networks essential for primordial follicle development. Transcriptional responses to eight different growth factors known to influence primordial follicles were used to construct a bionetwork of regulatory genes involved in primordial follicle development. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS4048

Platform:

GPL6247

44 Samples

Download data: CEL

Analysis of P4 ovaries of Sprague-Dawley females exposed to growth factors. Ovarian primordial follicles are critical for female reproduction and comprise a finite pool of gametes arrested in development. Results provide insight into molecular mechanisms underlying primordial follicle development.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 10 agent sets

Platform:

GPL6247

Series:

GSE20324

44 Samples

Download data: CEL

(Submitter supplied) Estradiol-17β (E2) has been shown to promote primordial follicle (PF) formation and to involve BMP2 as a downstream effector to promote PF in the hamsters. However, the molecular mechanism whereby these factors regulate ovarian somatic cells to pre-granulosa cells (preGC) transition leading to PF formation remains unclear. The objective of this study was to determine whether BMP2 and/or E2 would regulate the expression of specific ovarian transcriptome during preGC transition and PF formation in the mouse ovary. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: TXT

(Submitter supplied) To delineate the developmental trajectory of oocyte during the process, we performed RNA-seq on single germ cells from newborn (P0.5) ovaries and identified a series of genes that may function between stage transitions. The significant decrease of meiosis-related genes and the dramatic increase of oocyte-specific genes marked the transition of germ cell to a functional oocyte. Our data highlighted the complexity of transcriptional control and depicted the regulatons and their regulatory networks in oocyte development. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

146 Samples

Download data: TXT