GEO DataSets

(Submitter supplied) Whole genome high density tiliing array of eight timepoints from the promastitgote to amastigote differentiation process.

Organism:

Leishmania donovani; Leishmania infantum JPCM5

Type:

Expression profiling by array

Platform:

GPL10446

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Leishmania mexicana; Leishmania major

Type:

Expression profiling by array

Platform:

GPL3505

11 Samples

Download data: TXT

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. Statistical analysis on array hybridization data representing 8156 predicted coding regions revealed 288 genes (3.5% of all genes) whose steady-state mRNA levels meet criteria for differential regulation between promastigotes and lesion-derived amastigotes. more...

Organism:

Leishmania major; Leishmania mexicana

Type:

Expression profiling by array

Platform:

GPL3505

9 Samples

Download data: TXT

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. This experiment appears as Fig. 1 of the associated publication. Keywords: RNA expression profiling

Organism:

Leishmania major; Leishmania mexicana

Type:

Expression profiling by array

Platform:

GPL3505

4 Samples

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(Submitter supplied) This platform supports 11 60-mer DNA oligonucleotide probes synthesized twice on each slide for 8160 (98%) of the Leishmania major Friedlin genes annotated at www.genedb.org. Refer to: Timothy R. Holzer, W.R. McMaster, James D. Forney. “Expression profiling by whole-genome interspecies microarray hybridization reveals differential gene expression in procyclic promastigotes, lesion-derived amastigotes, and axenic amastigotes in Leishmania mexicana.” Molecular and Biochemical Parasitology 146 (2): 198-218. more...

Organism:

Leishmania major

3 Series

11 Samples

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(Submitter supplied) Histone acetylations are known to impact gene transcription. Here, we have attempted to examine the role of Leishmania donovani histone acetyltransferases HAT4 and HAT2 in regulating global gene expression. The transcriptome of HAT4-null promastigotes (Samples 2A, 2B) and HAT2-heterozygous knockout promastigotes (Samples 3A, 3B) have been compared with the transcriptome of wild-type Leishmania promastigotes (Samples 1A, 1B) and fold change in gene expression with respect to the control wild-type has been determined.

Organism:

Leishmania donovani

Type:

Expression profiling by array

Platform:

GPL18125

6 Samples

Download data: TXT, XLS

(Submitter supplied) In this study, we examined the transcriptome of Leishmania donovani promastigotes and axenic amastigotes to identify differentially regulated mRNAs utilizing the serial analysis of gene expression Keywords: stage differentiation; axenic amastigotes

Organism:

Leishmania donovani

Type:

Expression profiling by SAGE

Platform:

GPL3252

2 Samples

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(Submitter supplied) The ability of Leishmania to survive in their insect or mammalian host is dependent upon an ability to sense and adapt to changes in the microenvironment. However, little is known about the molecular mechanisms underlying the parasite response to environmental changes, such as nutrient availability. To elucidate nutrient stress response pathways in Leishmania donovani, we have used purine starvation as the paradigm. more...

Organism:

Leishmania donovani

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17379

2 Samples

Download data: TXT, XLSX

(Submitter supplied) Transcriptome analysis via high-throughput sequencing (RNA-Seq) captures significant changes in gene expression. The purpose of this study is to elucidate the transcriptomic changes in profilin deficient Leishmania promastigotes (LdPfn+/-) as compared to wild type control (LdPfn+/+). This research will enable us to gain insight into the role of profilin in Leishmania cells. The data has been validated by qRT-PCR. more...

Organism:

Leishmania donovani

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29657

6 Samples

Download data: TXT