GEO DataSets

(Submitter supplied) In order to find out the vital genes during the corneal neovascularization (CNV), we set up two CNV models, one being induced by suture placement and the other by alkali burn to the corneal center. Young adult Balb/c and C57BL/6 were used. The corneas were checked daily for CNV development and some of the corneas were removed at two pre-determined time points for RNA extraction. Preliminary experiment indicated slightly different dynamics in suture- or chemical burn-induced CNV. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10745

12 Samples

Download data: LSR

(Submitter supplied) When recruiting Balb/c mice for projects concerning corneas, we found that some mice presented corneal abnormality mimicking human corneal degenerations. The mice were unilaterally or bilaterally affected. The corneas of unilaterally affected mice were collected and pooled respectively into Normal and Degenerative samples. RNA was prepared and subjected to dual-labeling microarray analysis using the Capital Bio work station.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10745

2 Samples

Download data: LSR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platforms:

GPL21043 GPL21042

94 Samples

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(Submitter supplied) Recombinant-murine S100A9 were used at 10 mg/50 ml in Hanks Balanced Salt solution (HBSS) or control HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, or 12 h post-inhalation of S100A9. Because S100A9 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyze 49 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors.

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platform:

GPL21042

32 Samples

Download data: TXT

(Submitter supplied) LPS (10 μg/50 μl PBS; E. coli Serotype 055:B5, Sigma-Aldrich) was administered onto the nares. Baseline levels of genes in mice treated with vehicle (Hanks Balanced Salt solution (HBSS) and PBS), Intranasal S100s (10 μg/50 μl HBSS) were given 2 h before LPS; control mice received equal volumes of PBS and HBSS. Mice were sacrificed 4 h post LPS inhalation.

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platform:

GPL21043

40 Samples

Download data: TXT

(Submitter supplied) Recombinant-murine S100A8 were used at 10 mg/50 ml in Hanks Balanced Salt solution (HBSS) or control HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, or 12 h post-inhalation of S100A8. Because S100A8 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyze 49 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors.

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platform:

GPL21042

22 Samples

Download data: TXT

(Submitter supplied) Inflammation is a key component of pathological angiogenesis. Here we monitor gene expression profiles of the pre-sprouting phase of corneal angiogenesis in the rat model, as influenced by topically applied treatments. We used GeneChip Rat Genome 230 2.0 Array to monitor gene expression profies of several genes in the different treatment groups

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

16 Samples

Download data: CEL, CHP

(Submitter supplied) Previous studies showed that S100A8 and S100A9 are involved in neovascularization as well as in tumor development. At high concentrations, S100A8 and S100A9 cause inflammatory response or apoptosis mediated damage in vascular endothelial cells. But the effect of low concentrations of such proteins on endothelial cells remains unknown. This assay was performed to screen for genes that are involved in the response of Human Unbilican Vascular Endothelial Cells to low concentrations of S100A8.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

6 Samples

Download data: TXT

(Submitter supplied) MicroRNA profile comparison of the corneal endothelium of young and old mice: implications for senescence of the corneal endothelium

Organism:

Mus musculus; Murid betaherpesvirus 1; Murid gammaherpesvirus 4

Type:

Non-coding RNA profiling by array

Platform:

GPL17315

6 Samples

Download data: TXT, XLS

(Submitter supplied) Purpose: Klf5 plays a critical role in the mouse ocular surface (Kenchegowda et al., 2011. Dev Biol. 356:5-18). Here, we compare wild-type (WT) and Klf5-conditional null (Klf5CN) corneal gene expression at postnatal day-11 (PN11) and PN56 to identify the Klf5-target genes. Methods: Gene expression was compared using Affymetrix microarrays with QPCR validation. Transient transfection assays examined the effect of Klf5 on selected target gene promoter activities. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

14 Samples

Download data: CEL, TXT

(Submitter supplied) Neoadjuvant chemoradiation therapy (CRT) is a widely used preoperative treatment strategy for locally advanced rectal cancer (LARC). However, a few studies have evaluated the molecular changes caused by neoadjuvant CRT in these cancer tissues. Here, we aimed to investigate changes in immunotherapy-related immunogenic effects in response to preoperative CRT in LARC. We analyzed 60 pairs of human LARC tissues before and after irradiation from three independent LARC cohorts, including a LARC patient RNA sequencing (RNA-seq) dataset from our cohort and GSE15781 and GSE94104 datasets. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

22 Samples

Download data: TXT

(Submitter supplied) Although there have been studies conducted on cornea and retina growth and development, postnatal gene expression studies on sclera growth during postnatal growth has not been well characterised. Given that the mouse genome has 85% homology to the human genome and has been completely sequenced, mouse model for the study of ocular growth has advantages over other animal models. Thus, we aimed to study the biology and genetics behind sclera growth during post-natal development in Balb/cJ mice as a means to understand genetic changes that cause scleral growth and development during post-natal eye development The purpose of this study was to identify the genes underlying the development of mouse sclera post-natal growth of the posterior chamber of the eye using microarray

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

30 Samples

Download data: CEL

(Submitter supplied) Overall goal was to look for the differentially expressed genes between receptor activator of nuclear factor kappa-B ligand (RANKL)-stimulated wild type (WT) bone marrow macrophages (BMMs) and the BMMs in which the expression or the enzyme activity of cyclooxygenase-2 (Cox2), the major enzyme for prostaglandin E2 (PGE2) synthesis, is absent. For this purpose, two separate microarrays (experiment 1 and 2) were done. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

26 Samples

Download data: IDAT, TXT

(Submitter supplied) We report the application of chromatin immunoprecipitation sequencing to identify binding sites of the transcription factor serum response factor (SRF) in the cornea of WT and Dstn-corn1 mutant mice

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: BW, TXT, XLS

(Submitter supplied) The purpose of this study is to characterize gene expression changes that occur when conditional knock-out of Srf rescues mutant phenotypes in the cornea of Dstncorn1 mice. This study uses a unique model combining genetic and genomic approaches to identify genes that are regulated by SRF.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

9 Samples

Download data: CEL, CHP

(Submitter supplied) Comparative analysis was done to identify genes and pathways affected in the heart left ventricle during different stages of pregnancy. Microarray profiling was done on nonpregnant, pregnant, and early post-partum mice, with diestrus females serving as reference.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

8 Samples

Download data: CEL

(Submitter supplied) Housekeeping genes (HKG) are constitutively expressed in all tissues while tissue-enriched genes (TEG) are expressed at a much higher level in a single tissue type than in others. HKGs serve as valuable experimental controls in gene and protein expression experiments, while TEGs tend to represent distinct physiological processes and are frequently candidates for biomarkers or drug targets. The genomic features of these two groups of genes expressed in opposing patterns may shed light on the mechanisms by which cells maintain basic and tissue-specific functions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

26 related Platforms

564 Samples

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(Submitter supplied) Background Housekeeping genes (HKG) are constitutively expressed in all tissues while tissue-enriched genes (TEG) are expressed at a much higher level in a single tissue type than in others. HKGs serve as valuable experimental controls in gene and protein expression experiments, while TEGs tend to represent distinct physiological processes and are frequently candidates for biomarkers or drug targets. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3834

Platform:

GPL8217

42 Samples

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Analysis of 42 normal tissues. Housekeeping genes (HKG) are constitutively expressed in all tissues; tissue-enriched genes (TEG) are more highly expressed in a single tissue type than in others. Results provide insight into mechanisms by which cells maintain basic and tissue-specific functions.

Organism:

Homo sapiens

Type:

Expression profiling by array, log10 ratio, 42 tissue sets

Platform:

GPL8217

Series:

GSE14938

42 Samples

Download data

(Submitter supplied) Introduction: A number of genetic-association studies have identified genes contributing to AS susceptibility but such approaches provide little information as to the gene activity changes occurring during the disease process. Transcriptional profiling generates a “snapshot” of the sampled cells activity and thus can provide insights into the molecular processes driving the disease process. We undertook a whole-genome microarray approach to identify candidate genes associated with AS and validated these gene-expression changes in a larger sample cohort. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5231

Platform:

GPL6947

32 Samples

Download data: TXT